The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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The Effect of Samul-tanggahyangbuja on Depression and Learning on Repeated Stress in Ovariectomized Rats

사물탕가향부자(四物湯加香附子)가 난소적출 흰쥐의 우울 및 학습에 미치는 영향

  • Lee, Soon-Yee (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Kim, Song-Baek (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Seo, Yun-Jung (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Choi, Chang-Min (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University) ;
  • Cho, Han-Baek (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Won-Kwang University)
  • 이순이 (원광대학교 한의과대학 한방부인과학교실) ;
  • 김송백 (원광대학교 한의과대학 한방부인과학교실) ;
  • 서윤정 (원광대학교 한의과대학 한방부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 한방부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 한방부인과학교실)
  • Received : 2013.07.15
  • Accepted : 2013.08.19
  • Published : 2013.08.30
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Abstract

Objectives: In this research, the effect of samul-tanggahyangbuja on depression and learning in ovariectomized rats subjected to repetitive stress were assessed. Samul-tanggahyangbuja is the prescription consisting of Samul-tang and Cyperi Rhizoma. Methods: Ovariectomized rats were repeatedly stressed over a 2-week period. After being orally medicated with samul-tanggahyangbuja (100 or 400 mg/kg), rats performed the Morris water maze test and forced swimming test, and social exploration was assessed in a behavior test. As well, sucrose intake was measured and measurements of blood serum corticosterone and the change of interleukin-$1{\beta}$ (IL-$1{\beta}$) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in blood samples were made. Results: 1. In the Morris water maze test, rats medicated with 100 mg samul-tanggahyangbuja mastered the maze in a shorter time on the 4th day in comparison with the control group, while rats medicated with 400 mg samul-tanggahyangbuja mastered the maze more quickly (p<0.05 on the 3rd day ; p<0.01 on the 4th day, as compared to control). 2. Immobility time in the forced swimming test was significantly decreased in rats receiving 400 mg samul-tanggahyangbuja compared with the control group (p<0.05). 3. Sucrose intake and active social behavior of rats receiving 400 mg samul-tanggahyangbuja were markedly increased in comparison with the control group (p<0.01). 4. Blood serum corticosterone measurements revealed decreased blood serum corticosterone level after medicating with samul-tanggahyangbuja. But it was not statistically significant. 5. Treatment with either dose of samul-tanggahyangbuja significantly reduced IL-$1{\beta}$ and TNF-${\alpha}$ (p<0.05). Conclusions: These results suggest that samul-tanggahyangbuja possesses the anti-depressant and cognitive-enhancing activities related to menopause.

목 적: 본 연구에서는 부인과에서 빈용되고 있는 사물탕에 향부자를 가미한 처방을 사용하여 반복적인 스트레스를 가한 난소적출 흰쥐에 대하여 항우울 효과와 학습에 미치는 영향을 살펴보고자 하였다. 방 법: 난소적출 흰쥐에 2주간 반복적인 스트레스를 주고, 사물탕가향부자(100 mg/kg, 400 mg/kg)을 경구 투여한 후 행동검사로 Morris water maze test, Forced swimming test, Sucrose intake 측정과 Social exploration 관찰을 시행하였으며 혈액검사로 혈청 Corticosterone, IL-$1{\beta}$와 TNF-${\alpha}$의 변화를 측정하였다. 결 과: 1. Morris water maze test에서 사물탕가향부자 400 mg 투여군은 실험 3, 4일째 에 사물탕가향부자 100 mg 투여군은 실험 4일째에 acquisition trial 수행의 시간이 대조군에 비해서 단축되는 유의한 효과를 보였다. 2. Forced swimming test에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 immobility 시간이 유의하게 줄어들었다. 3. Sucrose intake test에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 현저히 자당 섭취량이 증가하였고, social exploration 관찰에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 현저히 active social behavior가 증가하였다. 4. Corticosterone 측정에서 사물탕가향부자를 투여한 후 corticosterone 수준이 감소하는 변화가 있었지만 유효하지는 않았다. 5. 사물탕가향부자 투여군(100 mg, 400 mg)에서 IL-$1{\beta}$와 TNF-${\alpha}$의 양이 대조군에 비해 유의하게 감소되었다. 결 론: 이상의 결과로 사물탕가향부자는 난소적출 흰쥐의 우울 및 인지력 저하에 유효함을 알 수 있었다.

Keywords

References

  1. The Korean Society of menopause Eds.The management of postmenopausal women. 3rd edition. Seoul:Koonja. 2007:1-5.
  2. The Korean Society of menopause Eds. Menopausal health. 2nd edition. Seoul:Koonja. 2000:55-61.
  3. Korean Society of Obestetric and Gynecology. Gynecology. 3rd edition. Seoul:Calvin. 1997:716-65.
  4. Parry BL. Perimenopausal depression. Am J Psychiatry. 2008;165(1):23-7. https://doi.org/10.1176/appi.ajp.2007.07071152
  5. Zhang ZJ. Geumgweyolyag. Seoul: Jeongdam. 2001:653.
  6. Oriental medicine Bangjehak classrooms. Bangjehak. Seoul:Youngrimsa. 1999: 287-8, 354-5.
  7. Lee SI. Tianjin prescription commentary. Seoul:Seougbosa. 1987:53-60.
  8. Seong WY, et al. The effects of Gwibi -tang in the chronic mild stress model of depression in rats. J of Oriental Neuropsychiatry. 2002;13(2):121-47.
  9. Lee JA, et al. The effects of Gwibi -tang on an animal model of depression induced by chronic mild stress. J of Oriental Neuropsychiatry. 2001;12(1) :123-35.
  10. Lee KS, et al. The antidepressant effect of Nelumbinis semen on forced swimming test in the rats. Korean Journal of Stress Research. 2008; 16(2):99-106.
  11. Hong YY, et al. A study on melatonin, serotonin secretion change and behavior in the rats treated with Yiseon-tang, Gammakdaejo-tang, Sanjoin-tang and Sanjogammak-tang. The Journal of Oriental Obstetrics ＆ Gyencology. 1999;12(1):209-29.
  12. Kim YS, et al. Antidepressive effects of Soyo-san on ovariectomized rats. The Journal of Oriental Obstetrics ＆ Gyencology. 2001;14(2):69-84.
  13. Moon HJ, et al. Influence of Gammakdaejo -Tang on brain Fos-like immunoreactivity in response to immobilization stress in ovariectomized rats. The Journal of Oriental Obstetrics ＆ Gynecology. 2002;15(2):25-40.
  14. Cryan JF, et al. Assessing antidepressant activity rodents: recent developments and future needs. Trends Pharmacol Sci. 2002;23(5):238-45. https://doi.org/10.1016/S0165-6147(02)02017-5
  15. Lucki I. The forced swimming test as a model for core and component behavioral effects of antidepressant drugs. Behav Pharmacol. 1997;8(6-7): 523-32. https://doi.org/10.1097/00008877-199711000-00010
  16. The Korean Society of Oriental Neuropsychiatry Eds. Herbal medicine neuropsychiatry. Paju:Jipmoondang. 2005:488, 482-95.
  17. Oriental Gynecology Textbook Complication Committee. Oriental gynecology. Seoul: Jeongdam. 2001:218-44.
  18. Shors TJ, Leuner B. Estrogen-mediated effects on depression and memory formation in females. J Affect Disord. 2003;74(1):85-96. https://doi.org/10.1016/S0165-0327(02)00428-7
  19. Frick KM, et al. Estrogen replacement improves spatial reference memory and increases hippocampal synaptophysin in aged female mice. Neuroscience. 2002;115(2):547-58. https://doi.org/10.1016/S0306-4522(02)00377-9
  20. Chen SW. Taepyeonghyeminhwajegugbang. Beijing:People's Health Publishers. 1985:310-1.
  21. Kim JJ, et al. Anti-inflammatory action by Gamisangryosamul-tang and the effect of Ziyang-go on atopic dermatitis-like lesion and pruritus in NC/Nga mice. Korean J Oriental Physiology ＆ Pathology. 2003;17(2):428-35.
  22. Kim EK, et al. Effect of Samul-tang on the allergic inflammatory response. Korean J Oriental Physiology ＆ Pathology. 2007;21(3):617-25.
  23. Bae SH, Nam CG. Effects of Samul-tang and constituent herbs on a contracted artery of rabbit. Korean J Orient Int Med. 2000;21(1):23-30.
  24. Kim YH, Cho HB. Anti-inflammatory effects of Haedogsamul-tang in RAW 264.7 cells. The Journal of Oriental Obstetrics ＆ Gyencology. 2008;21(2) :166-83.
  25. Chang SH, et al. Effects of Dohongsamul -tang on surgically induced endometriosis in rats. The Journal of Oriental Obstetrics ＆ Gynecology. 2006;19(1) :125-38.
  26. Lee HY, et al. Clinical study about the effects of Gamisamul-tang on lactogenesis and prolactin in early puerperal period. The Journal of Oriental Obstetrics ＆ Gynecology. 2004;17(2):147-56.
  27. Jeong YS, et al. Effect of Gamisamul -tang on hyperlipidemia in ovariectomized rats. Korean J Oriental Physiology ＆ Pathology. 2002;16(1):89-94.
  28. Heo Joon. Source dongeuibogam. Seoul :Namsandang. 2001;156, 159, 162, 604.
  29. Lee YH, Park YJ. Antidepressants and cognitive function. J Korean Society of Biological Therapies in Psychatry. 2002;8(1):186-97.
  30. D'Hooge R, De Deyn PP. Application of the Morris water maze in the study of learning and memory. Brain Research Reviews. 2001;36(1):60-90. https://doi.org/10.1016/S0165-0173(01)00067-4
  31. Kim HT, et al. Checking for neuroscientists encephalopathy animal behavior. Seoul :Sigma press. 2006:14-7, 64-5.
  32. Kurtuncu M, et al. Reliability assessment of an automated forced swim test device using two mouse strains. Journal of Neuroscience Methods. 2005;149(1) :26-30. https://doi.org/10.1016/j.jneumeth.2005.04.010
  33. De Pablo JM, et al. Learned immobility explains the behavior of rats in the forced swimming test. Physiology & Behavior. 1989;46(2):229-37. https://doi.org/10.1016/0031-9384(89)90261-8
  34. Gronli J, et al. Chronic mild stress affects sucrose intake and sleep in rats. Behavioural Brain Research. 2004;150(1-2):139-47. https://doi.org/10.1016/S0166-4328(03)00252-3
  35. Seckl JR, et al. Diurnal variation of plasma corticosterone in depression. Psychoneuroendocrinology. 1990;15(5-6) :485-8. https://doi.org/10.1016/0306-4530(90)90071-G
  36. Stamp J, Herbert J. Corticosterone modulates autonomic responses and adaptation of central immediate-early gene expression to repeated restraint stress. Neuroscience. 2001;107(3):465-79. https://doi.org/10.1016/S0306-4522(01)00364-5
  37. Goshen I, Yirmiya R. Interleukin-1 (IL-1) : A central regulator of stress responses. Frontiers in Neuroendocrinology. 2009;30:30-45. https://doi.org/10.1016/j.yfrne.2008.10.001
  38. McAfoose J, Baune BT. Evidence for a cytokine model of cognitive function. Neuroscience & Biobehavioral Reviews. 2009;33(3):355-66. https://doi.org/10.1016/j.neubiorev.2008.10.005