Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Effects of Mineral Trioxide Aggregate on the Proliferation and Differentiation of Human Dental Pulp Stromal Cells from Permanent and Deciduous Teeth

Mineral trioxide aggregate가 유치 및 영구치의 치수기질세포 증식 및 분화에 미치는 영향

  • Kim, Seunghye (Department of Pediatric Dentistry, College of Dentistry and Oral Science Research Center, Yonsei University) ;
  • Jeon, Mijeong (Department of Pediatric Dentistry, College of Dentistry and Oral Science Research Center, Yonsei University) ;
  • Shin, Dong Min (Department of Oral Science, College of Dentistry, Yonsei University) ;
  • Lee, Jae Ho (Department of Pediatric Dentistry, College of Dentistry and Oral Science Research Center, Yonsei University) ;
  • Song, Je Seon (Department of Pediatric Dentistry, College of Dentistry and Oral Science Research Center, Yonsei University)
  • 김승혜 (연세대학교 치과대학 소아치과학교실, 구강과학연구소) ;
  • 전미정 (연세대학교 치과대학 소아치과학교실, 구강과학연구소) ;
  • 신동민 (연세대학교 치과대학 구강생물학교실) ;
  • 이제호 (연세대학교 치과대학 소아치과학교실, 구강과학연구소) ;
  • 송제선 (연세대학교 치과대학 소아치과학교실, 구강과학연구소)
  • Received : 2012.11.30
  • Accepted : 2013.06.11
  • Published : 2013.08.30
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Abstract

Mineral trioxide aggregate (MTA) has recently been used as a pulpotomy medicament for primary molars. The aim of this study was to evaluate and compare the proliferation and differentiation potential of dental pulp stromal cells of permanent teeth and deciduous teeth cultured on MTA-coated surface. Human dental pulp stromal cells were obtained from human permanent premolars and deciduous teeth and cultured on MTA-coated culture plates. The cells were subjected to proliferation assay and cell cycle analysis. Their differentiation potential was evaluated by analysing changes in the mRNA expressions of runt-related transcriptional factor 2 (Runx2) and alkaline phosphatase (ALP). Morphological changes of cells in direct contact with MTA were observed using scanning electron microscopy (SEM). The proliferation rates, distribution of cell cycles and mRNA expression patterns of Runx2 and ALP were similar in both types of pulpal cells. SEM observations revealed that both types changed into more dendrite-like cells. On the surface of MTA, human dental pulp stromal cells from deciduous and permanent teeth were able to both proliferate and differentiate into cells that induce mineralization. MTA is suitable as a biocompatible pulpotomy medicament for primary teeth.

최근 유구치의 치수절단술 약제로 MTA의 임상 적용이 문헌들에서 보고된 바 있으나 MTA 표면에서 일어나는 유치 치수 세포의 반응에 대한 시험관내 연구는 많이 보고되지 않았다. 이번 연구의 목적은 유치 및 영구치에서 유래한 치수기질세포가 경화된 MTA 표면에서 나타내는 증식 및 분화 능력을 비교 평가하는 것이었다. 사람 영구치와 유치 치수 조직에서 분리된 치수기질세포를 경화된 MTA 표면에서 배양 후 세포증식율과 세포주기를 검사하였으며, 정량적 역전사 중합효소 연쇄반응(RT-PCR)을 사용하여 분화양상을 분석하였다. Runt-related transcription factor 2(Runx2)와 alkaline phosphatase(ALP)가 정량적 RT-PCR의 표지자로 사용되었고, MTA 표면에서 증식된 치수기질세포의 형태학적 변화를 주사전자현미경 하에서 관찰하였다. 영구치와 유치의 치수기질세포군은 세포증식률, 세포주기 분포 및 mRNA 발현 양상에 있어서 차이를 보이지 않았으며, 주사전자현미경 상에서 두 군 모두 수지상 형태를 나타내었다. MTA 상에서 관찰된 유치와 영구치의 치수기질세포의 비슷한 증식력 및 광화를 유도하는 세포로의 분화능은 유치의 치수절단술 제재로 MTA가 생체친화적으로 적합함을 보여준다.

Keywords

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