Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effects of Trichostatin A on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

  • Diao, Yun-Fei (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Kenji, Naruse (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Han, Rong-Xun (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Lin, Tao (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Oqani, Reza-K. (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Kang, Jung-Won (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Jin, Dong-Il (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
  • Received : 2013.05.10
  • Accepted : 2013.06.08
  • Published : 2013.06.30
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Abstract

Developmental potential of cloned embryos is related closely to epigenetic modification of somatic cell genome. The present study was to investigate the effects of applying histone deacetylation inhibitor, trichostatin A (TSA) to activated porcine embryos on subsequent development of porcine parthenogenetic and nuclear transfer embryos. Electrically activated oocytes were treated with 5 nM TSA for different exposure times (0, 1, 2 and 4 hr) and then the activated embryos were cultured for 7 days. The reconstructed embryos were treated with different concentrations of 0, 5, 10 and 25 nM TSA for 1 hr. Also 5 nM TSA was tested with different exposure times of 0, 0.5, 1, 2 and 4 hr. And fetal fibroblast cells were treated with 50 nM TSA for 1, 2 or 4 hr and with 5 nM TSA for 1 hr. Cumulus-free oocytes were enucleated and reconstructed by TSA-treated donor cells and electrically fused and cultured for 6 days. In parthenogenetic activation experiments, 5 nM TSA treatment for 1 hr significantly improved the percentage of blastocyst developmental rates than the other groups. Total cell number of blastocysts in 1 hr group was significantly higher than other groups or control. Similarly, blastocyst developmental rates of porcine NT embryos following 5 nM TSA treatment for 1 hr were highest. And the reconstructed embryos from donor cells treated by 50 nM TSA for 1 hr improved the percentage of blastocyst developmental rates than the control group. In conclusion, TSA treatment could improve the subsequent blastocyst development of porcine parthenogenetic and nuclear transfer embryos.

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References

  1. Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C, Williams JL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade HM, Godke RA, Gavin WG, Overstrom EW, Echelard Y (1999): Production of goats by somatic cell nuclear transfer. Nature Biotechnology 17:456-461. https://doi.org/10.1038/8632
  2. Chesne P, Adenot PG, Viglietta C, Baratte M, Boulanger L, Renard JP (2002): Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat Biotechnol 20:366-369. https://doi.org/10.1038/nbt0402-366
  3. Dannenberg LO, Edenberg HJ (2006): Epigenetics of gene expression in human hepatoma cells: expression profiling the response to inhibition of DNA methylation and histone deacetylation. BMC Genomics 7:181. https://doi.org/10.1186/1471-2164-7-181
  4. Enright BP, Kubota C, Yang X, Tian XC (2003): Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin A or 5-aza-2'-deoxycytidine. Biol Reprod 69:896-901. https://doi.org/10.1095/biolreprod.103.017954
  5. Galli C, Lagutina I, Crotti G, Colleoni S, Turini P, Ponderato N, Duchi R, Lazzari G (2003): Pregnancy: a cloned horse born to its dam twin. Nature 424:635.
  6. Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H, Tsunoda Y (1998): Eight calves cloned from somatic cells of a single adult. Science 282:2095-2098. https://doi.org/10.1126/science.282.5396.2095
  7. Kishigami S, Thuan NV, Hikichi T, Ohta H, Wakayama S, Mizutani E, Wakayama T (2006a): Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids. Dev Biol 289:195-205. https://doi.org/10.1016/j.ydbio.2005.10.026
  8. Kishigami S, Mizutani E, Ohta H, Hikichi T, Thuan NV, Wakayama S, Bui HT, Wakayama T (2006b): Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem Biophys Res Commun 340: 183-189. https://doi.org/10.1016/j.bbrc.2005.11.164
  9. Li Z, Engelhardt JF (2003): Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer. Reprod Biol Endocrinol 1:83. https://doi.org/10.1186/1477-7827-1-83
  10. Niemann H, Wrenzycki C (2000): Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: implications for subsequent development. Theriogenology 53:21-34. https://doi.org/10.1016/S0093-691X(99)00237-X
  11. Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H, Perry AC (2000): Pig cloning by microinjection of fetal fibroblast nuclei. Science 289:1188-1190. https://doi.org/10.1126/science.289.5482.1188
  12. Parekh-Olmedo H, Engstrom JU, Kmiec EB (2003): The effect of hydroxyurea and trichostatin A on targeted nucleotide exchange in yeast and mammalian cells. Ann N Y Acad Sci 1002:43-55. https://doi.org/10.1196/annals.1281.006
  13. Park KW, Cheong HT, Lai L, Im GS, Kuhholzer B, Bonk A, Samuel M, Rieke A, Day BN, Murphy CN, Carter DB, Prather RS (2001): Production of nuclear transfer-derived swine that express the enhanced green fluorescent protein. Anim Biotech 12: 173-181. https://doi.org/10.1081/ABIO-100108344
  14. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH (2000): Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:505-509.
  15. Reik W, Dean W, Walter J (2001): Epigenetic reprogramming in mammalian development. Science 293: 1089-1093. https://doi.org/10.1126/science.1063443
  16. Rideout WM 3rd, Eggan K, Jaenisch R (2001): Nuclear cloning and epigenetic reprogramming of the genome. Science 293:1093-1098. https://doi.org/10.1126/science.1063206
  17. Shin T, Kraemer D, Pryor J, Liu L, Rugila J, Howe L, Buck S, Murphy K, Lyons L, Westhusin M (2002): A cat cloned by nuclear transplantation. Nature 415:859. https://doi.org/10.1038/nature723
  18. Shi W, Zakhartchenko V, Wolf E (2003): Epigenetic reprogramming in mammalian nuclear transfer. Differentiation 71:91-113. https://doi.org/10.1046/j.1432-0436.2003.710201.x
  19. Svensson K, Mattsson R, James TC, Wentzel P, Pilartz M, MacLaughlin J, Miller SJ, Olssen T, Eriksson UJ, Ohlsson R (1998): The paternal allele of the H19 gene is progressively silenced during early mouse development: the acetylation status of histones may be involved in the generation of variegated expression patterns. Development 125:61-69.
  20. Tanaka S, Oda M, Toyoshima Y, Wakayama T, Tanaka M, Yoshida N, Hattori N, Ohgane J, Yanagimachi R, Shiota K (2001): Placentomegaly in cloned mouse concepti caused by expansion of the spongiotrophoblast layer. Biol Reprod 65:1813-1821. https://doi.org/10.1095/biolreprod65.6.1813
  21. Thompson EM, Legouy E, Christians E, Renard JP (1995): Progressive maturation of chromatin structure regulates HSP70.1 gene expression in the preimplantation mouse embryo. Development 121:3425- 3437.
  22. Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374. https://doi.org/10.1038/28615
  23. Wee G, Koo DB, Song BS, Kim JS, Kang MJ, Moon SJ, Kang YK, Lee KK, Han YM (2006): Inheritable histone H4 acetylation of somatic chromatins in cloned embryos. J Biol Chem 281:6048-6057.
  24. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997): Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813. https://doi.org/10.1038/385810a0
  25. Woods GL, White KL, Vanderwall DK, Li GP, Aston KI, Bunch TD, Meerdo LN, Pate BJ (2003): A mule cloned from fetal cells by nuclear transfer. Science 301:1063. https://doi.org/10.1126/science.1086743
  26. Yoshioka K, Suzuki C, Tanaka A, Anas IM, Iwamura S (2002): Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol Reprod 66:112-119. https://doi.org/10.1095/biolreprod66.1.112
  27. Zhang Y, Li J, Villemoes K, Pedersen AM, Purup S, Vajta G (2007): An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transfer. Cloning and Stem Cells 9:357- https://doi.org/10.1089/clo.2006.0090
  28. Zhou Q, Renard JP, Le Friec G, Brochard V, Beaujean N, Cherifi Y, Fraichard A, Cozzi J (2003) : Generation of fertile cloned rats by regulating oocyte activation. Science 302:1179. (Received: 10 May 2013/ Accepted: 8 June 2013) https://doi.org/10.1126/science.1088313