KSBB Journal

  • 제28권2호
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  • Pages.146-150
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  • 2013
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
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유독 이온성 액체의 장기 노출에 대한 Shewanella oneidensis MR-1의 반응: MR-1과 순응된 균주 SH-1의 비교 연구

Response of Shewanella oneidensis MR-1 to Longterm Exposure with Toxic Ionic Liquid: Comparative Studies on MR-1 and SH-1, an Acclimated Strain

  • Han, Sang Hyun (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Kang, Chang-Ho (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Kwak, Dae Yung (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Ha, Sung Ho (Department of Chemical Engineering and NanoBioTechnology, Hannam University) ;
  • Koo, Yoon-Mo (Department of Biological Engineering, College of Engineering, Inha University) ;
  • So, Jae-Seong (Department of Biological Engineering, College of Engineering, Inha University)
  • 투고 : 2012.12.23
  • 심사 : 2013.04.09
  • 발행 : 2013.04.27
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초록

In this study, we investigated the effects of longterm exposure to ionic liquid (IL) on Shewanella oneidensis MR-1 (MR-1). MR1 was acclimated through repeated exposure to IL. The acclimated strain was named as S. oneidensis SH-1 (SH-1) and compared with MR-1 in various aspects including morphology, cell surface hydrophobicity (CSH), motility, and fatty acid. Compared to the MR-1, SH-1 showed elongated cell shape on scanning electron microscopy. Upon exposure to IL, hydrophobicity of SH-1 (28.2%) was higher that of MR-1 (3.3%). In contrast, motility of SH-1 (7 mm) was lower than that also of MR-1 (22 mm), and branched chain fatty acid of SH-1 was lower than that of MR-1, 27.6% and 41.1%, respectively.

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참고문헌

  1. Plechkova, N. V. and K. R. Seddon (2008) Applications of ionic liquids in the chemical industry. Chem. Soc. Rev. 37: 123-150. https://doi.org/10.1039/b006677j
  2. Matsumoto, M., K. Mochiduki, and K. Kondo (2004) Toxicity of ionic liquids and organic solvents to lactic acid producing bacteria. J. Biosci. Bioeng. 98: 344-347. https://doi.org/10.1016/S1389-1723(04)00293-2
  3. Lee, S. M., W. J. Chang, A. R. Choi, and Y. M. Koo (2005) Influence of ionic liquids on the growth of Escherichia coli. Korean J. Chem. Eng. 22: 687-690. https://doi.org/10.1007/BF02705783
  4. Azimova, M. A., S. A. Morton, and P. D. Frymier (2009) Comparison of three bacterial toxicity assays for imidazolium derived ionic liquids. J. Environ. Eng. 135: 1388-1392. https://doi.org/10.1061/(ASCE)EE.1943-7870.0000092
  5. Bernot, R. J., M. A. Brueseke, M. A. E. White, and G. A. Lamberti (2005) Acute and chronic toxicity of imidazolium-based ionic liquids on Daphnia magna. Environ. Toxicol. Chem. 24: 87-92. https://doi.org/10.1897/03-635.1
  6. Cho, C. W., T. P. T. Pham, Y. C. Jeon, K. Vijayaraghavan, W. S. Choe, and Y. S. Yun (2007) Toxicity of imidazolium salt with anion bromide to a phytoplankton Selenastrum capricornutum: Effect of alkylchain length. Chemosphere. 69: 1003-1007. https://doi.org/10.1016/j.chemosphere.2007.06.023
  7. National Toxicology Program (NTP) and National Institute of Environmental Health Sciences (NIEHS) (2004) "Review of Toxicological Literature for Ionic Liquids", prepared by Integrated Laboratory Systems Inc., Research Triangle Park, NC, USA.
  8. Fredrickson, J. K., M. F. Romine, A. S. Beliaev, J. M. Auchtung, M. E. Driscoll, T. S. Gardner, K. H. Nealson, A. L. Osterman, G. Pinchuk, J. L. Reed, D. A. Rodionov, J. L. M. Rodrigues, D. A. Saffarini, M. H. Serres, A. M. Spormann, I. B. Zhulin, and J. M. Tiedje (2008) Towards environmental systems biology of Shewanella. Nat. Rev. Microbiol. 6: 592-603. https://doi.org/10.1038/nrmicro1947
  9. Brown, S. D., M. Martin, S. Deshpande, S. Seal, K. Huang, E. Alm, Y. Yang, L. Wu, T. Yan, X. Liu, A. Arkin, K. Chourey, J. Zhou, and D. K. Thompson (2006) Cellular Response of Shewanella oneidensis to Strontium Stress. Appl. Environ. Microbiol. 72: 890-900. https://doi.org/10.1128/AEM.72.1.890-900.2006
  10. Boukhalfa, H., G. A. Icopini, S. D. Reilly, and M. P, Neu (2007) Plutonium (IV) Reduction by the MetalReducing Bacteria Geobacter metallireducens GS-15 and Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 73: 5897-5903. https://doi.org/10.1128/AEM.00747-07
  11. Hau H. H. and J. A. Gralnick (2007) Ecology and biotechnology of the genus Shewanella. Annu. Rev. Microbiol. 61: 237-258. https://doi.org/10.1146/annurev.micro.61.080706.093257
  12. Heidelberg, J. F., I. T. Paulsen, K. E. Nelson, E. J. Gaidos, W. C. Nelson, T. D. Read, J. A. Eisen, R. Seshadri, N. Ward, B. Methe, R. A. Clayton, T. Meyer, A. Tsapin, J. Scott, M. Beanan, L. Brinkac, S. Daugherty, R. T. DeBoy, R. J. Dodson, A. S. Durkin, D. H. Haft, J. F. Kolonay, R. Madupu, J. D. Peterson, L. A. Umayam, O. White, A. M. Wolf, J. Vamathevan, J. Weidman, M. Impraim, K. Lee, K. Berry, C. Lee, J. Mueller, H. Khouri, J. Gill, T. R. Utterback, L. A. McDonald, T. V. Feldblyum, H. O. Smith, J. C. Venter, K. H. Nealson, and C. M. Fraser (2002) Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis. Nat. Biotech. 20: 1118-1123. https://doi.org/10.1038/nbt749
  13. Zhang, Y. and R. M. Miller (1994) Effect of a Pseudomonas Rhamnolipid Biosurfactant on Cell Hydrophobicity and Biodegradation of Octadecane. Appl. Environ. Microbiol. 60: 2101-2106.
  14. Al-Tahhan, R. A., T. R. Sandrin, A. A. Bodour, and R. M. Maier (2000) RhamnolipidInduced removal of Lipopolysaccharide from Pseudomonas aeruginosa: Effect on cell surface properties and interaction with hydrophobic substrates. Appl. Environ. Microbiol. 66: 3262-3268. https://doi.org/10.1128/AEM.66.8.3262-3268.2000
  15. Miller, L. T. (1982) Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy. J. Clin. Microbiol. 16: 584-586.
  16. Zhang, C., S. V. Malhotra, and A. J. Francis (2011) Toxicity of imidazolium and pyridiniumbased ionic liquids and the cometabolic degradation of Nethylpyridinium Tetrafluoroborate. Chemosphere. 82: 1690-1695. https://doi.org/10.1016/j.chemosphere.2010.10.085
  17. Aertsen, A., R. Van Houdt, K. Vanoirbeek, and C. W. Michiels (2004) An SOS response induced by high pressure in Escherichia coli. J. Bacteriol. 186: 6133-6141. https://doi.org/10.1128/JB.186.18.6133-6141.2004
  18. Lindahl, M., M. Faris, T. Wadstrom, and S. Hjerten (1981) A new test based on 'salting out' to measure relative hydrophobicity of bacterial cells. Biochim. Biophys. Acta. 677: 471-476. https://doi.org/10.1016/0304-4165(81)90261-0
  19. Rosenberg, M. (2006) Microbial adhesion to hydrocarbons: twentyfive years of doing MATH. FEMS Microbiol. Lett. 262: 129-134. https://doi.org/10.1111/j.1574-6968.2006.00291.x
  20. Liu, Y., W. Gao, Y. Wang, L. Wu, X. Liu, T. Yan, E. Alm, A. Arkin, D. K. Thompson, M. W. Fields, and J. Zhou (2005) Transcriptome analysis of Shewanella oneidensis MR-1 in response to elevated salt conditions. J. Bacteriol. 187: 2501-2507. https://doi.org/10.1128/JB.187.7.2501-2507.2005
  21. Kaneda, T. (1991) Iso- and Anteiso-Fatty Acids in Bacteria: Biosynthesis, Function, and Taxonomic Significancet. Microbiol. Rev. 55: 288-302.