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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Silkworm Gland Hydrolysate on Albumin-erythropoietin Production in Transgenic Chinese Hamster Ovary Cells

형질전환 Chinese Hamster Ovary 세포에서 Albumin-erythropoietin의 생산시 Silkworm Gland Hydrolysate의 효과

  • Choi, Min-Ho (Department of Biological Engineering, Inha University) ;
  • Cha, Hyun-Myoung (Department of Biological Engineering, Inha University) ;
  • Kim, Sun-Mi (Department of Applied Bioscience, CHA University) ;
  • Choi, Yong-Soo (Department of Applied Bioscience, CHA University) ;
  • Kim, Dong-Il (Department of Biological Engineering, Inha University)
  • 최민호 (인하대학교 공과대학 생물공학과) ;
  • 차현명 (인하대학교 공과대학 생물공학과) ;
  • 김선미 (차의과학대학교 생명과학대학 바이오공학과) ;
  • 최용수 (차의과학대학교 생명과학대학 바이오공학과) ;
  • 김동일 (인하대학교 공과대학 생물공학과)
  • Received : 2013.02.15
  • Accepted : 2013.03.12
  • Published : 2013.04.27
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Abstract

To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26- fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.

Keywords

References

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