Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Antibiotic Properties of Helicosporium sp. KCTC 0635BP to Rhizoctonia solani AG2-2 IV

Rhizoctonia solani AG2-2 IV에 대한 Helicosporium sp. KCTC 0635BP의 항균활성

  • Lee, Sang Myeong (Major of Applied Biology, School of Ecological Environment and Tourism, Kyungpook National University) ;
  • Kim, Dong Soo (Southern Forest Resources Research Center, Korea Forest Research Institute) ;
  • Lee, Kwang-Soo (Southern Forest Resources Research Center, Korea Forest Research Institute) ;
  • Lee, Chong-Kyu (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Lee, Dong Woon (Major of Applied Biology, School of Ecological Environment and Tourism, Kyungpook National University)
  • 이상명 (경북대학교 생태환경관광학부 생물응용전공) ;
  • 김동수 (국립산림과학원 남부산림자원연구소) ;
  • 이광수 (국립산림과학원 남부산림자원연구소) ;
  • 이총규 (경남과학기술대학교 산림자원학과) ;
  • 이동운 (경북대학교 생태환경관광학부 생물응용전공)
  • Received : 2013.06.10
  • Accepted : 2013.06.18
  • Published : 2013.06.30
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Abstract

Biocontrol potential of an isolate of Helicosporium spp. against Rhizoctonia solani, Fusarium oxysporium and Phytophthora drechsleri was evaluated in vitro and in vivo. A selected biocontrol agent designated as Helicosporium 0635BP strongly inhibited growth and lysed mycelium of Rhizoctonia solani and Fusarium oxysporium on PDA. Autoclaved culture filtrate of the agent also completely inhibited growth of the turfgrass large patch pathogen, R. solani AG2-2 IV at the concentration of 50 ml $L^{-1}$. The pathogen was killed when dipped under the 20% filtrate for four hours or 50% for one hr. In a field trial, plots applied with the crude or times diluted culture filtrate showed 100% control efficacy of the turfgrass large patch as a chemical applied for a comparison. Results indicated that Helicosporium 0635BP is a promising biocontrol agent on control of the turfgrass large patch disease and its culture filtrate contained unknown heat suitable antifungal substance (s). Further studies on mass production, purification and identification of the unknown compound (s) are in progress for practical use.

Helicosporium spp.의 식물병원균 Rhizoctonia solani와 Fusarium oxysporium, Phytophthora drechsleri에 대한 항균 활성을 실내와 야외에서 검정하였다. Helicosporium 0635BP 균주는 Rhizoctonia solani와 Fusarium oxysporium에 대해 PDA배지상에서 강한 균사억제 효과를 보였다. 살균한 배양여액 50 ml/L 처리 시 잔디 갈색퍼짐병균(Rhizoctonia solani AG2-2)에 대한 높은 생장억제효과를 보였다. 배양여액 20%에 4시간 동안 침지하거나 50% 배양여액에 1시간 침지 시 Rhizoctonia solani AG2-2 IV는 100% 사멸하였다. 골프장 들잔디에서 Helicosporium 0635BP 균주의 배약여액 처리 시 10배액까지 잔디 갈색퍼짐병의 병 진전을 대조약제와 동일하게 100% 억제하였다. 따라서 Helicosporium 0635BP 배양액은 잔디 갈색퍼짐병의 생물적 방제인자로 활용가능 할 것으로 생각되며 실용화를 위하여 대량배양과 항균 물질의 정제와 동정이 필요할 것으로 보인다.

Keywords

References

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