Asian Pacific Journal of Cancer Prevention

  • 제14권2호
  • /
  • Pages.669-672
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  • 2013
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  • 1513-7368(pISSN)
  • /
  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Expression and Significance of Twist and E-cadherin in Ovarian Cancer Tissues

  • Wang, Wen-Shuang (Department of Gynecology, Yantai Yuhuangding Hospital) ;
  • Yu, Shou-Li (Department of Gynecology, Yantai Yuhuangding Hospital) ;
  • Yang, Xing-Sheng (Department of Gynecology, Qilu Hospial of Shandong University) ;
  • Chang, Shu-De (Department of Gynecology, Yantai Affiliated Hospital of Binzhou Medical College) ;
  • Hou, Jian-Qing (Department of Gynecology, Yantai Yuhuangding Hospital)
  • 발행 : 2013.02.28
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초록

Objective: To investigate the expression of Twist and E-cadherin in ovarian cancer tissues as well as the role of epithelial-mesenchymal transformation (EMT) in ovarian cancer metastasis. Method: The expressions of Twist and E-cadherin in 54 cases of ovarian cancer and paracancerous tissues were detected by Western blottin g and reverse transcriptase polymerase chain reaction. We used RNA interference to silence Twist expression in human ovarian cancer cell line, and detected E-cadherin expression using Western blotting. Results: There was an increase in the relative abundance of Twist proteins and a decrease in E-cadherin in ovarian cancer compared with normal ovary tissues (P < 0.05). The expression levels of Twist and E-cadherin mRNA were $1.49{\pm}0.53$ and $0.82{\pm}0.24$ in ovarian cancer, and $1.14{\pm}0.38$ and $1.08{\pm}0.19$ in paracancerous tissues, respectively. The difference between the indicators in ovarian cancer and in paracancerous tissues was statistically significant (P < 0.05). When the Twist expression was silenced in an ovarian cancer cell line, the expression of the E-cadherin protein increased (P<0.05). Conclusion: The expression of Twist is upregulated, whereas that of E-cadherin is downregulated in ovarian cancer. EMT, mediated by Twist, may be correlated with ovarian cancer metastasis.

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참고문헌

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