Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immunomodulatory and Antigenotoxic Properties of Bacillus amyloliquefaciens KU801

면역조절능과 유전독성 억제능을 가지는 Bacillus amyloliquefaciens KU801

  • Lee, Na-Kyoung (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University) ;
  • Kim, So-Yeon (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University) ;
  • Chang, Hyo-Ihl (School of Life Sciences and Biotechnology, Korea University) ;
  • Park, Eunju (Department of Food Nutrition, Kyungnam University) ;
  • Paik, Hyun-Dong (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University)
  • 이나경 (건국대학교 동물생명과학대학 생명분자정보학센터) ;
  • 김소연 (건국대학교 동물생명과학대학 생명분자정보학센터) ;
  • 장효일 (고려대학교 생명환경과학대학) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 백현동 (건국대학교 동물생명과학대학 생명분자정보학센터)
  • Received : 2013.01.23
  • Accepted : 2013.04.03
  • Published : 2013.06.28
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Abstract

The Bacillus KU801 strain, due to its potential in the field of probiotics for animal use, was isolated from chicken feces. Strain KU801 was identified as Bacillus amyloliquefaciens KU801 based on the results of 16S rRNA sequencing. Vegetative and spore cells of B. amyloliquefaciens KU801 were resistant to artificial gastric juice and artificial bile acid. B. amyloliquefaciens KU801 was found to inhibit the production of nitric oxide (NO) and increase the production of Interleukin-1 alpha (IL-1${\alpha}$). DNA damage induced by N-methyl-Ntion of ninitroso-guanidine (MNNG) was significantly inhibited, in a dose dependent manner, by preincubating MNNG together with B. amyloliquefaciens KU801. These results demonstrate the potential use of B. amyloliquefaciens KU801 as a feed additive.

닭분변으로부터 면역활성능이 뛰어난 KU801 균주를 분리하고 16S rRNA 서열분석을 통해 Bacillus amyloliquefaciens KU801로 동정하였다. B. amyloliquefaciens KU801의 영양세포와 아포세포는 인공위액 (pH 2.5, 1% pepsin)과 인공담즙 (0.3% oxgall)에 대한 저항성을 나타내었다. B. amyloliquefaciens KU801은 산화질소 (NO)의 생산을 감소시켰으나 인터루킨-$1{\alpha}$ (IL-$1{\alpha}$)의 생산은 증가시키는 것을 확인하였다. Commet assay를 통한 유전독성능에 미치는 영향을 확인한 결과, B. amyloliquefaciens KU801을 첨가하였을 때 DNA 손상을 처리 농도에 비례하여 감소시키는 것을 확인하였다. 이들 결과를 토대로, B. amyloliquefaciens KU801은 사료용 정장제로서의 이용 가능성을 확인할 수 있었다.

Keywords

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