Journal of Plant Biotechnology

  • 제40권2호
  • /
  • Pages.72-78
  • /
  • 2013
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

절편부위와 사이토키닌이 갓의 재분화에 미치는 영향

Effects of different types and ages of explants and cytokinins on shoot regeneration in Brassica juncea L.

  • 노경희 (국립농업과학원 농업생명자원부) ;
  • 곽보경 (국립농업과학원 농업생명자원부) ;
  • 김종범 (국립농업과학원 농업생명자원부) ;
  • 이경렬 (국립농업과학원 농업생명자원부) ;
  • 김현욱 (국립농업과학원 농업생명자원부) ;
  • 김순희 (국립농업과학원 농업생명자원부) ;
  • 강한철 (국립농업과학원 농업생명자원부)
  • Roh, Kyung Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kwak, Bo-Kyung (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Jong-Bum (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Lee, Kyeong-Ryeol (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Hyun Uk (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Sun Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kang, Han Chul (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA)
  • 투고 : 2013.04.29
  • 심사 : 2013.05.25
  • 발행 : 2013.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 갓(Brassica juncea L. Czern)의 효율적 재분화조건 확립을 위해 재분화가 잘되는 절편체 종류와 절편체의 나이 그리고 재분화율 향상에 관여하는 사이토키닌 종류와 적정 함량을 조사하였다. 절편체 종류에 따른 갓의 재분화율은 다음과 같았다: 잎자루 달린 자엽> 하배축> 잎자루 달린 본엽> 자엽> 본엽. 또한 파종 후 5일된 잎자루 달린 자엽에서의 재분화능이 가장 양호하였다. 6종의 사이토키닌 중에서- 6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine (2-ip), 6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine riboside (2-ip riboside), 6-Benzyl amino-purine (BAP), Thidiazuron (TDZ), Zeatin, Zeatin riboside-TDZ $8{\mu}M$ 처리구에서 재분화율이 평균 80%로 가장 높은 것을 알 수 있었다. 이렇게 획득한 재분화 식물체는 온실로 옮겨져 재배되었으며, 정상적인 생육과정을 거쳐 개화가 이루어지는 것이 관찰되었다.

To establish an efficient protocol for plant regeneration of Brassica juncea L. Czern, the effects of explant types, explant ages and cytokinins on shoot regeneration were examined in this study. Shoot regeneration was markedly affected by the explant types used in the following order: cotyledon with petiole> hypocotyl> leaf with petiole> cotyledon> leaf. Five-day-old seedlings of cotyledon with petiole explants showed the maximum shoot regeneration frequency. Of the six cytokinins-6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine (2-ip), 6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine riboside (2-ip riboside), 6-Benzyl amino-purine (BAP), Thidiazuron (TDZ), Zeatin, Zeatin riboside-TDZ ($8{\mu}M$) was found to be the best cytokinin for shoot regeneration with the highest shoot induction frequency (80%) from cotyledon with petiole after 4 weeks. All the regenerated plantlets were developed well and they produced morphologically normal flowers.

키워드

참고문헌

  1. 농촌진흥청 (2003) 표준영농교본. In: 엽채류 재배, Vol. 140, 삼미기획, pp 111-116
  2. Agarwal PK, Ranu RS (2000) Regeneration of plantlets from leaf and petiole explants of Pelargonium X Hortorum. In Vitro Cell Dev Biol-Plant 36:392-397 https://doi.org/10.1007/s11627-000-0070-y
  3. Akasaka-Kennedy Y, Yoshida H, Takahata Y (2005) Efficient plant regeneration from leaves of rapeseed (Brassica napus L.): the influence of AgNO3 and genotype. Plant Cell Rep 24:649-654 https://doi.org/10.1007/s00299-005-0010-8
  4. Babic V, Datla RS, Scoles GJ, Keller WA (1998) Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata L. Plant Cell Rep 17:183-188 https://doi.org/10.1007/s002990050375
  5. Bhuiyan MSU, Min SR, Choi KS, Lim YP, Liu JR (2009) Factors for high frequency plant regeneration in tissue cultures of Indian mustard (Brassica juncea L.). J Plant Biotechnol 36:137-143 https://doi.org/10.5010/JPB.2009.36.2.137
  6. Burnett L, Arnoldo M, Yarrow S, Huang B (1994) Enhancement of shoot regeneration from cotyledon explants of Brassica rapa spp oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors. Plant Cell Tiss Org Cult 37:253-256
  7. Cardoza V, Stewart CN (2004) Brassica biotechnology: Progress in cellular and molecular biology. In Vitro Cell Dev Biol- Plant 40:542-551 https://doi.org/10.1079/IVP2004568
  8. Guo DP, Zhu ZJ, Hu XX, Zheng SJ (2005) Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai). Plant Cell Tiss Organ Cult 83:123-127 https://doi.org/10.1007/s11240-005-3799-5
  9. Haberlandt G (1902) Kulturversuche mit isolierten Pflanzenzellen Sitz. Akad Wiss Wien 111:69-92
  10. Hemingway JS (1976) Mustards, Brassica spp. and Sinapis alba (Cruciferae) In: Simmonds NW, (eds), Evolution of Crop Plants, Longman, New York, pp 19-21
  11. Keresa S, Mihovilovic A, Baric M, Jercic IH (2011) Efficient plant regeneration of Begonia semperflorens and Begonia spp. from petiole and leaf explants. JFAE 9:240-244
  12. Kim H, Kim JY, Kim HJ, Kim DK, Jo HJ, Han BS, Kim HW, Kim JB (2011) Anticancer activity and quantitative analysis of Glucosinolates from green and red leaf mustard. Korea J Food & Nutr 24:362-366 https://doi.org/10.9799/ksfan.2011.24.3.362
  13. Kumar N, Reddy MP (2010) Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Ann Appl Biol 156:367-375 https://doi.org/10.1111/j.1744-7348.2010.00394.x
  14. Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol 29:92-96 https://doi.org/10.1007/BF02632259
  15. Maheshwari P, Selvaraj G, Kovalchuk I (2011) Optimization of Brassica napus (canola) explants regeneration for genetic transformation. Nature Biotechnology 29:144-155
  16. Mithen R (2001) Glucosinolates-biochemistry, genetics and biological activity. Plant Growth Regulation 34:91-103 https://doi.org/10.1023/A:1013330819778
  17. Moloney MM, Walker JM, Sharma KK (1989) High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep 8:238-242 https://doi.org/10.1007/BF00778542
  18. Muller C, Sieling N (2006) Effects of glucosinolates and myrosinase levels in Brassica juncea on a glucosinolates- sequestering herbivore-and vice versa. Chemioecology 16:191-201 https://doi.org/10.1007/s00049-006-0347-7
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-493 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  20. Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267-275 https://doi.org/10.1007/BF02822732
  21. Nada S, Chennareddy S, Goldman S (2011) Direct shoot bud differentiation and plantlet regeneration from leaf and petiole explants of Begonia tuberhybrida. HortSceince 46:759-764
  22. Oram RN, Kirk JTO, Veness PE, Hurlstone CJ, Edlington JP,Halsall DM (2005) Breeding Indian mustard (Brassica juncea(L.) Czern.) for cold-pressed, edible oil production-a review. Aust J Agr Res 56:581-596 https://doi.org/10.1071/AR04295
  23. Park SH, Choi SI, Park JB, Han HK, Bae SD, Sung IJ, Park ER (2011) Phytoremediation on the heavy metal contaminated soil by hyperaccumulators in the greenhouse. J Soil & Groundwater Env 16:1-8
  24. Potts DA, Rakow GW, Males DR (1999) Canola-Quality Brassica juncea, a New Oilseed Crop for the Canadian Prairies. Proceedings of the 10th International Rapeseed Congress, Canberra, Australia
  25. Radke SE, Turner JC, Facciotti D (1992) Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens. Plant Cell Rep 11:499-505
  26. Raskin I, Smith RD, Salt DE (1997) Phytoremediation of metals: using plants to remove pollutants from the environment. Curr Opin Biotech 8:221-226 https://doi.org/10.1016/S0958-1669(97)80106-1
  27. Roh KH, Kwak BK, Kim HU, Lee KR, Kim SH, Suh MC, Kim H, Kim JB (2011) Production of transgenic plants in Brassica napus winter cultivar 'Youngsan'. J Appl Biol Chem 54:26-32 https://doi.org/10.3839/jabc.2011.005
  28. Roh KH, Kwak BK, Kim JB, Lee KR, Kim HU, Kim SH (2012) The influence of silver thiosulfate and thidiazuron on shoot regeneration from cotyledon explants of Brassica napus. J Plant Biotechnol 39:133-139 https://doi.org/10.5010/JPB.2012.39.3.133
  29. Schilperoort RA, Veldstra H, Warnaar SQ, Mulder G, Cohen JA (1967) Formation of complexes between DNA isolated from tobacco crown gall tumours and complementary to Agrobacterium tumefaciens DNA. Biochim Biophys Acta 145:523-525 https://doi.org/10.1016/0005-2787(67)90075-5
  30. Spect CE, Diederichsen A (2001) Brassica. In: Hanelt P, (eds), Mansfeld's Encyclopedia of Agricultural and Horticultural Crops, Vol. 6, Springer-Verlag, Berlin, pp 1453-1456
  31. Takagi H, Sugawara S, Saito T, Tasaki H, Yuanxue L, Kaiyun G, Han DS, Godo T, Nakano M (2011) Plant regeneration via direct and indirect adventitious shoot formation and chromosome- doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder. Plant Biotechnol Rep 5:187-195 https://doi.org/10.1007/s11816-011-0172-5
  32. Tang GX, Zhou WJ, Li HZ, Mao BZ, He ZH, Yoneyama K (2003) Medium, explants and genotype factors influencing shoot regeneration in oilseed Brassica spp. J Agronomy & Crop Science 189:351-358 https://doi.org/10.1046/j.1439-037X.2003.00060.x
  33. van Staden J, Zazimalova E, George EF (2008) Plant growth regulators II: Cytokinins, their analogues and antagonists. In Geroge EF, Hall MA, De Kerke GJ, (eds), Plant propagation by tissue culture, Ed 3, Vol. 1, Springer, Dordrecht, pp 205-226
  34. Wright PR, Morgan JM, Jessop RS (1997) Turgor maintenance by osmoregulation in Brassica napus and B. juncea under field conditions. Ann Bot 80:313-319 https://doi.org/10.1006/anbo.1997.0444
  35. Yang M, Lee M (2008) Rhizofiltration process with Helianthus annuss L., Phaseolus vulgaris var., and Brassica juncea (L.) Czern. to remediate uranium contaminated groundwater. J KoSSGE 13:30-39
  36. Zhang FL, Takahata Y, and Xu JB (1998) Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep 17:780-786 https://doi.org/10.1007/s002990050482
  37. Zhang Y, Talalay P, Cho CG, Posner GH (1992) A major inducer of anticarcinogenic protective enzymes from broccoli: Isolation and elucidation of structure. Proc Natl Acad Sci 89:2399-2403 https://doi.org/10.1073/pnas.89.6.2399
  38. Zhao J, Cui J, Liu J, Liao F, Henny RJ, Chen J (2012) Direct somatic embryogenesis from leaf and petiole explants of Spathiphyllum 'Supreme' and analysis of regenerants using flow cytometry. Plant Cell Tiss Org Cult 110:239-249 https://doi.org/10.1007/s11240-012-0146-5