Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of different types and ages of explants and cytokinins on shoot regeneration in Brassica juncea L.

절편부위와 사이토키닌이 갓의 재분화에 미치는 영향

  • Roh, Kyung Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kwak, Bo-Kyung (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Jong-Bum (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Lee, Kyeong-Ryeol (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Hyun Uk (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Sun Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kang, Han Chul (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA)
  • 노경희 (국립농업과학원 농업생명자원부) ;
  • 곽보경 (국립농업과학원 농업생명자원부) ;
  • 김종범 (국립농업과학원 농업생명자원부) ;
  • 이경렬 (국립농업과학원 농업생명자원부) ;
  • 김현욱 (국립농업과학원 농업생명자원부) ;
  • 김순희 (국립농업과학원 농업생명자원부) ;
  • 강한철 (국립농업과학원 농업생명자원부)
  • Received : 2013.04.29
  • Accepted : 2013.05.25
  • Published : 2013.06.30
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Abstract

To establish an efficient protocol for plant regeneration of Brassica juncea L. Czern, the effects of explant types, explant ages and cytokinins on shoot regeneration were examined in this study. Shoot regeneration was markedly affected by the explant types used in the following order: cotyledon with petiole> hypocotyl> leaf with petiole> cotyledon> leaf. Five-day-old seedlings of cotyledon with petiole explants showed the maximum shoot regeneration frequency. Of the six cytokinins-6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine (2-ip), 6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine riboside (2-ip riboside), 6-Benzyl amino-purine (BAP), Thidiazuron (TDZ), Zeatin, Zeatin riboside-TDZ ($8{\mu}M$) was found to be the best cytokinin for shoot regeneration with the highest shoot induction frequency (80%) from cotyledon with petiole after 4 weeks. All the regenerated plantlets were developed well and they produced morphologically normal flowers.

본 연구는 갓(Brassica juncea L. Czern)의 효율적 재분화조건 확립을 위해 재분화가 잘되는 절편체 종류와 절편체의 나이 그리고 재분화율 향상에 관여하는 사이토키닌 종류와 적정 함량을 조사하였다. 절편체 종류에 따른 갓의 재분화율은 다음과 같았다: 잎자루 달린 자엽> 하배축> 잎자루 달린 본엽> 자엽> 본엽. 또한 파종 후 5일된 잎자루 달린 자엽에서의 재분화능이 가장 양호하였다. 6종의 사이토키닌 중에서- 6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine (2-ip), 6-${\gamma}$-${\gamma}$-Dimethylallylamino-purine riboside (2-ip riboside), 6-Benzyl amino-purine (BAP), Thidiazuron (TDZ), Zeatin, Zeatin riboside-TDZ $8{\mu}M$ 처리구에서 재분화율이 평균 80%로 가장 높은 것을 알 수 있었다. 이렇게 획득한 재분화 식물체는 온실로 옮겨져 재배되었으며, 정상적인 생육과정을 거쳐 개화가 이루어지는 것이 관찰되었다.

Keywords

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