Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Analysis of Active Components in Cirsium japonicum var. ussuriense Extracts and Their Effect on TGF-beta Induced Hepatic Stellate Cells Activation

엉겅퀴 추출물의 기능 성분 분석 및 TGF-beta에 의한 간 성상 세포 활성 억제 효과

  • 김선영 ((재)전주생물소재연구소) ;
  • 김상준 ((재)전주생물소재연구소) ;
  • 최영지 ((재)전주생물소재연구소) ;
  • 유강열 ((재)전주생물소재연구소) ;
  • 정창호 ((재)전주생물소재연구소) ;
  • 심재석 (임실생약조합) ;
  • 장선일 (전주대학교 대체의학대학) ;
  • 유동현 (전라북도농업기술원 특화작목연구소) ;
  • 정승일 ((재)전주생물소재연구소)
  • Received : 2013.04.25
  • Accepted : 2013.05.21
  • Published : 2013.06.30
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Abstract

Cirsium japonicum (CJ) leaf (L) alcoholic extracts were investigated for analysis their active components (flavonoids and flavanolignans; silymarins) and inhibitory effect on transforming growth factor (TGF)-${\beta}$ induced hepatic stellate cells (HSCs, LX-2 cells) activation. The CJ root (R) extracts were also analyzed and compared with leaf extracts. Total flavonoid and polyphenol contents of the leaf extracts showed higher than those of the root extracts. The content of each flavonoid compound, which was analyzed by HPLC, in CJ-L extracts was also higher than in CJ-R extracts. The results of flavanolignans content in CJ-L and CJ-R extracts were consistent in flavonoid and polyphenol. We studied inhibitory effect of two extracts against TGF-${\beta}1$ induced HSCs activation. The CJ-L extracts significantly suppressed overexpression of profibrogenic factor, ${\alpha}$-smooth muscle actin and collagen-${\alpha}1$(I). The CJ-R extract also showed inhibitory effect on TGF-${\beta}1$ induced HSCs activation, but the efficacy was lower than in CJ-L extract. These results suggest that CJ-L may contribute to the fibrotic liver treatment.

Keywords

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