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Safety Evaluation of 30 kGy Irradiated Chocolate Ice Cream

30 kGy 감마선 조사된 초코아이스크림의 안전성 평가

  • Jeon, Young Eun (Dept. of Food Science and Nutrition and The Korean Institute of Nutrition, Hallym University) ;
  • Yin, Xing Fu (Dept. of Food Science and Nutrition and The Korean Institute of Nutrition, Hallym University) ;
  • Chung, Cha-Kwon (Dept. of Food Science and Nutrition and The Korean Institute of Nutrition, Hallym University) ;
  • Kang, Il-Jun (Dept. of Food Science and Nutrition and The Korean Institute of Nutrition, Hallym University)
  • 전영은 (한림대학교 식품영양학과.한국영양연구소) ;
  • 윤성복 (한림대학교 식품영양학과.한국영양연구소) ;
  • 정차권 (한림대학교 식품영양학과.한국영양연구소) ;
  • 강일준 (한림대학교 식품영양학과.한국영양연구소)
  • Received : 2013.02.18
  • Accepted : 2013.03.18
  • Published : 2013.06.30

Abstract

This study was investigated the potential toxicity of gamma-irradiated chocolate ice cream for its future use in space. Chocolate ice cream was irradiated at a dose of 30 kGy at a temperature of $-20^{\circ}C$. For the animal study, AIN-93G was used as a control diet and irradiated and non-irradiated chocolate ice cream diets were administered to male and female ICR mice (ten mice per group) for three months. During the experimental period, the group fed irradiated chocolate ice cream did not show any changes in appearance, behavior, mortality, body weight, organ weight, or food consumption compared to the control. Also, all biochemical parameters, including hematology profiles, erythrocyte counts, and serum biochemical values were in normal ranges. In histopathological examinations of liver and kidney tissues, there were no significant differences between the control group and the group fed irradiated chocolate ice cream. These results indicate that chocolate ice cream irradiated at 30 kGy did not cause any toxic effects and could be applied for the development of safe and hygienic space food.

본 연구는 초코아이스크림의 안전성을 확보하기 위해서 30 kGy 조사된 초코아이스크림을 ICR 마우스에 90일간 섭취시킨 다음 독성평가를 수행하였다. 비 조사 시료 및 30 kGy 조사 초코아이스크림을 암수 마우스에 투여를 한 결과 시험기간 동안 시험 물질에 의한 임상증상이나 폐사 동물을 나타나지 않았으며, 체중변화, 사료섭취량 및 주요 장기 무게도 대조군에 비해 차이를 보이지 않았다. 혈액학적 검사 및 혈청학적 검사 모두 정상적인 수치를 나타내었다. 병리조직학적 검사 역시 간 및 신장 모두 정상적인 구조를 유지하고 있었으며 염증, 괴사 등의 유의할만한 병적 변화도 관찰되지 않았다. 따라서 30 kGy로 감마선 조사된 초코아이스크림은 암수 마우스에 3개월간 섭취시켜도 본 시험조건에서 독성이 없는 것으로 판명되었다.

Keywords

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