Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Analysis of the antibiotic resistance gene in Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province

경북지역 환돈 유래 Salmonella Typhimurium의 약제내성 유전자 분석

  • Kim, Joo-Hyung (Gyeongbuk Veterinary Service Laboratory) ;
  • Kim, Seong-Guk (Gyeongbuk Veterinary Service Laboratory Northern Branch) ;
  • Kim, Seon-Soo (Gyeongbuk Veterinary Service Laboratory Northern Branch) ;
  • Kim, Jeong-Hwa (Gyeongbuk Veterinary Service Laboratory Northern Branch) ;
  • Park, Sye-Hee (Gyeongbuk Veterinary Service Laboratory Northern Branch) ;
  • Nam, Ki-Hu (Gyeongbuk Veterinary Service Laboratory Northern Branch) ;
  • Kim, Hyoung-Bae (Gyeongbuk Veterinary Service Laboratory Northern Branch)
  • 김주형 (경상북도가축위생시험소) ;
  • 김성국 (경상북도가축위생시험소 북부지소) ;
  • 김선수 (경상북도가축위생시험소 북부지소) ;
  • 김정화 (경상북도가축위생시험소 북부지소) ;
  • 박세희 (경상북도가축위생시험소 북부지소) ;
  • 남기후 (경상북도가축위생시험소 북부지소) ;
  • 김형배 (경상북도가축위생시험소 북부지소)
  • Received : 2013.02.14
  • Accepted : 2013.04.11
  • Published : 2013.06.30
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Abstract

This study was conducted to investigate antibiotic resistance among Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province during the period 1998~2011. One hundred forty one isolates were tested for antibiotic resistance using the standard disk diffusion method and were examined for presence of resistance gene by PCR method. S. Typhimurium showed high drug resistance rates to tetracycline (95.7%), streptomycin (93.6%), ampicillin (86.5%), cephalothin (80.1%), gentamicin (79.4%), and trimethoprim/sulfamethoxazole (72.3%). Resistance gene, blaTEM, blaPSE1, tetA, tetB, tetG, sul1, sul2, aadA, strA, grm, and temA were detected among the antibiotic resistance isolates and temB, tetC, aadB gene were not detected. One hundred twenty one (89.6%) tetA, two (1.5%) tetB and one (0.7%) tetG gene were detected in the 135 tetracycline resistant isolates. Two (1.6%) temA gene were detected in one hundred twenty two ampicillin resistance isolates and temB was not detected.

Keywords

References

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