Journal of Periodontal and Implant Science

  • 제43권1호
  • /
  • Pages.24-29
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  • 2013
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
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Correlation of expression and activity of matrix metalloproteinase-9 and -2 in human gingival cells of periodontitis patients

  • Kim, Kyung-A (Department of Orthodontics, Kyung Hee University School of Dentistry) ;
  • Chung, Soo-Bong (Department of Orthodontics, Kyung Hee University School of Dentistry) ;
  • Hawng, Eun-Young (Department of Orthodontics, Kyung Hee University School of Dentistry) ;
  • Noh, Seung-Hyun (Department of Orthodontics, Kyung Hee University School of Dentistry) ;
  • Song, Kwon-Ho (Department of Biological Science, Molecular and Cellular Glycobiology Unit, Sungkyunkwan University) ;
  • Kim, Hanna-Hyun (College of Art and Sciences, New York University) ;
  • Kim, Cheorl-Ho (Department of Biological Science, Molecular and Cellular Glycobiology Unit, Sungkyunkwan University) ;
  • Park, Young-Guk (Department of Orthodontics, Oral Biology Research Institute, Kyung Hee University School of Dentistry)
  • 투고 : 2012.12.04
  • 심사 : 2013.01.18
  • 발행 : 2013.02.28
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초록

Purpose: Matrix metalloproteinases (MMPs) are capable of degrading extracellular matrix, and they are inducible enzymes depending on an inflammatory environment such as periodontitis and bacterial infection in periodontal tissue. Gingival inflammation has been postulated to be correlated with the production of MMP-2 and MMP-9. The objective of this study was to quantify the expression and activity of MMP-9 and -2, and to determine the correlation between activity and expression of these MMPs in human gingival tissues with periodontitis. Methods: The gingival tissues of 13 patients were homogenized in $500{\mu}L$ of phosphate buffered saline with a protease inhibitor cocktail. The expression and activity of MMP-2 and -9 were measured by enzyme-linked immunosorbent assay and Western blot analysis, and quantified by a densitometer. For the correlation line, statistical analysis was performed using the Systat software package. Results: MMP-9 was highly expressed in all gingival tissue samples, whereas MMP-2 was underexpressed compared with MMP-9. MMP-9 activity increased together with the MMP-9 expression level, with a positive correlation (r=0.793, P=0.01). The correlation was not observed in MMP-2. Conclusions: The expression of MMP-2 and -9 might contribute to periodontal physiological and pathological processes, and the degree of MMP-9 expression and activity are predictive indicators relevant to the progression of periodontitis.

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과제정보

연구 과제 주관 기관 : Korea National Research Foundation

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  7. Interleukin‐25 regulates matrix metalloproteinase‐2 and ‐9 expression in periodontal fibroblast cells through ERK and P38MAPK pathways vol.44, pp.11, 2020, https://doi.org/10.1002/cbin.11430