Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
권호 표지

Simultaneous Enrichment of Novel Filamentous-Like Bacterial Population in Lab-Scale Granular Anaerobic Ammonia Oxidation (Anammox) Sequencing Batch Reactor

실험실 규모 입상 혐기성 암모늄 산화 연속회분식 반응조 내의 신종 사상균 동시 농화 배양에 관한 연구

  • Park, Hongkeun (Department of Earth and Environmental Engineering, Columbia University)
  • 박홍근 (콜럼비아대학교 지구환경공학과)
  • Published : 2013.05.30
⟨ Previous Next ⟩

Abstract

Enriching anammox bacteria (AMX) in a lab-scale granular sequencing batch reactor using local digester centrate, we observed the significant enrichment of the filamentous-like bacterial population. These bacteria were revealed as novel bacterial species (termed CHL) belonging to Chlorobi/Bacteroidetes phyla via Denaturing Gradient Gel Electrophoresis (DGGE). Further, niche differentiation of AMX and CHL quantification was observed in granule and filament biomass, suggesting AMX was dominant in the granule and CHL was dominant in the filament. Therefore, it was confirmed the structural role of CHL was indeed to aid the granule formation of the AMX. In parallel, the physiological role of CHL was suspected to degrade biopolymers in the digester centrate using nitrate as an electron acceptor.

Keywords

References

  1. Bellucci, M. and Curtis, T. P. (2011). Chapter Eleven - Ammonia-Oxidizing Bacteria in Wastewater, Methods in Enzymology, Martin, G. K., Lisa, Y. S., Eds. Academic Press, (496), pp. 269-286.
  2. Cho, S., Takahashi, Y., Fujii, N., Yamada, Y., Satoh, H., and Okabe, S. (2010). Nitrogen Removal Performance and Microbial Community Analysis of an Anaerobic Up-flow Granular Bed Anammox Reactor, Chemosphere, 78(9), pp. 1129-1135. https://doi.org/10.1016/j.chemosphere.2009.12.034
  3. Cole, J. R., Wang, Q., Cardenas, E., Fish, J., Chai, B., Farris, R. J., Kulam-Syed-Mohideen, A. S., McGarrell, D. M., Marsh, T., Garrity, G. M., Tiedje, J. M. (2009). The Ribosomal Database Project: Improved Alignments and New Tools for rRNA Analysis, Nucleic Acids Research, 37, pp. 141-145.
  4. Ferris, M. J., Muyzer, G., and Ward, D. M. (1996). Denaturing Gradient Gel Electrophoresis Profiles of 16S rRNA-defined Populations Inhabiting a Hot Spring Microbial Mat Community, Applied and Environmental Microbiology, 62(2), pp. 340-346.
  5. Hu, Z., Lotti, T., de Kreuk, M., Kleerebezem, R., van Loosdrecht, M., Kruit, J., Jetten, M. S. M., and Kartal, B. (2013). Nitrogen Removal by a Nitritation-anammox Bioreactor at Low Temperature, Applied and Environmental Microbiology, Published Ahead of Print, doi: 10.1128/AEM.03987-12.
  6. Jukes, T. H. and Cantor, C. R. (1969). Evolution of Protein Molecules, Mammalian Protein Metabolism, Munro, H. N., Ed. Academic Press, New York, pp. 21-132.
  7. Kindaichi, T., Yuri, S., Ozaki, N., and Ohashi, A. (2012). Ecophysiological role and Function of Uncultured Chloroflexi in an Anammox Reactor, Water Science And Technology, 66(12), pp. 2556-2561. https://doi.org/10.2166/wst.2012.479
  8. Kumar, S., Tamura, K., and Nei, M. (2004). Integrated Software for Molecular Evolutionary Genetics Analysis and Sequence Alignment, Briefings in Bioinformatics, 5, pp. 150-163. https://doi.org/10.1093/bib/5.2.150
  9. Li, X. R., Du, B., Fu, H. X., Wang, R. F., Shi, J. H., Wang, Y., Jetten, M. S. M., and Quan, Z. X. (2009). The Bacterial Diversity in an Anaerobic Ammonium-oxidizing (anammox) Reactor Community, Systematic and Applied Microbiology, 32(4), pp. 278-289. https://doi.org/10.1016/j.syapm.2009.03.002
  10. Liu, T., Li, D., Zeng, H., Li, X., Zeng, T., Chang, X., Cai, Y., and Zhang, J. (2012a). Biodiversity and Quantification of Functional Bacteria in Completely Autotrophic Nitrogenremoval Over Nitrite (CANON) Process, Bioresource Technology, 118, pp. 399-406. https://doi.org/10.1016/j.biortech.2012.05.036
  11. Liu, Z., Frigaard, N. U., Vogl, K., Iino, T., Ohkuma, M., Overmann, J., and Bryant, D. A. (2012b). Complete Genome of Ignavibacterium Album, a Metabolically Versatile, Flagellated, Facultative Anaerobe from the Phylum Chlorobi, Frontiers in Microbiology, 3, pp. 185.
  12. Ma, C., Jin, R. C., Yang, G. F., Yu, J. J., Xing, B. S., and Zhang, Q. Q. (2012). Impacts of Transient Salinity Shock Loads on Anammox Process Performance, Bioresource Technology, 112, pp. 124-130. https://doi.org/10.1016/j.biortech.2012.02.122
  13. Park, H., Rosenthal, A., Jezek, R., Ramalingam, K., Fillos, J., and Chandran, K. (2010a). Impact of Inocula and Growth Mode on the Molecular Microbial Ecology of Anaerobic Ammonia Oxidation (anammox) Bioreactor Communities, Water Research, 44(17), pp. 5005-5013. https://doi.org/10.1016/j.watres.2010.07.022
  14. Park, H., Rosenthal, A., Ramalingam, K., Fillos, J., and Chandran, K. (2010b). Linking Community Profiles, Gene Expression and N-removal in Anammox Bioreactors Treating Municipal Anaerobic Digestion Reject Water, Environmental Science & Technology, 44(16), pp. 6110-6116. https://doi.org/10.1021/es1002956
  15. Podosokorskaya, O. A., Kadnikov, V. V., Gavrilov, S. N., Mardanov, A. V., Merkel, A. Y., Karnachuk, O. V., Ravin, N. V., Bonch-Osmolovskaya, E. A., and Kublanov, I. V. (2013). Characterization of Melioribacter Roseus Gen. nov., sp. nov., a Novel Facultatively Anaerobic Thermophilic Cellulolytic Bacterium from the Class Ignavibacteria, and a Proposal of a Novel Bacterial Phylum Ignavibacteriae, Environmental Microbiology, Published Ahead of Print, doi:10.1111/1462-2920.12067.
  16. Rozen, S. and Skaletsky, H. (2000). Primer3 on the WWW for General Users and for Biologist Programmers, Methods in Molecular Biology, 132, pp. 365-86.
  17. Strous, M., Pelletier, E., Mangenot, S., Rattei, T., Lehner, A., Taylor, M. W., Horn, M., Daims, H., Bartol-Mavel, D., Wincker, P., Barbe, V., Fonknechten, N., Vallenet, D., Segurens, B., Schenowitz-Truong, C., Medigue, C., Collingro, A., Snel, B., Dutilh, B. E., Op den Camp, H. J. M., van der Drift, C., Cirpus, I., van de Pas-Schoonen, K. T., Harhangi, H. R., van Niftrik, L., Schmid, M., Keltjens, J., van de Vossenberg, J., Kartal, B., Meier, H., Frishman, D., Huynen, M. A., Mewes, H. W., Weissenbach, J., Jetten, M. S. M., Wagner, M., and Le Paslier, D. (2006). Deciphering the Evolution and Metabolism of an Anammox Bacterium from a Community Genome, Nature, 440(7085), pp. 790-794. https://doi.org/10.1038/nature04647
  18. van der Star, W., Abma, W. R., Blommers, D., Mulder, J. W., Tokutomi, T., Strous, M., Picioreanu, C., and van Loosdrecht, M. C. M. (2007). Startup of Reactors for Anoxic Ammonium Oxidation: Experiences from the First Full-scale Anammox Reactor in Rotterdam, Water Research, 41(18), pp. 4149-4163. https://doi.org/10.1016/j.watres.2007.03.044