Influence of Thickness of Optical Panel on the Growth Rate of Chlorella vulgaris in Photobioreactor

광생물반응기에서 도광판의 두께가 Chlorella vulgaris 증식에 미치는 영향

  • Published : 2013.03.30

Abstract

The aim of this study was to investigate the efficiency of thickness of optical panel (OP) on the growth rate of Chlorella vulgaris. The size of Chlorella vulgaris (FC-16) was $3-8{\mu}m$, having round in shape. The cells of Chlorella vulgaris was cultured in the Jaworski's Medium with deionized water at $22^{\circ}C$ for 15 days. For this experiment, three OP samples were prepared to evaluate the efficiency of thickness of OP on the growth rate of Chlorella vulgaris; 4 mm OP with LED (Light Emitting Diode) (Run 1), 6 mm OP with LED (Run 2) and 8 mm with LED (Run 3). The diffuse rate was reached 86%, 91% and 92% for Run 1, Run 2 and Run 3, respectively. Average biomass of Run 2 and Run 3 were measured 11.18% higher than that of Run 1. However, the specific growth rate for all fractions were almost same. In addition, chlorophyll content per cell and cell volume were found to be slice difference between Run 2 and Run 3. Therefore, Run 2 has more effect on growth rate of biomass for Chlorella vulgaris than Run 1 and Run 3.

Keywords

References

  1. Alain, D., Jean, D., Francoise, P., and Lhoussaine, B. (2000). Growth Rate Four Freshwater Algae in Relation to Light and Temperature, Hydrobiologia, 207(1), pp. 221-226.
  2. Chen, C. Y., Yeh, K. L., Aisyah, R., Lee, D. J., and Chang, J. S. (2011). Cultivation, Photobioreactor Design and Harvesting of Microalgae for Biodiesel Production: A Critical Review, Bioresource Technology, 102(1), pp. 71-81. https://doi.org/10.1016/j.biortech.2010.06.159
  3. Chen, X., Goh, Y. Q., Tan, W., Hossain, I., Chen, W. N., and Lau, R. (2011). Lumostatic Strategy for Microalgae Cultivation Utilizing Image Analysis and Chlorophyll a Content as Design Parameters, Bioresource Technology, 102(10), pp. 6005-6012. https://doi.org/10.1016/j.biortech.2011.02.061
  4. Chisti, Y. (2007). Biodiesel from Microalgae, Biotechnology Advances, 25(3), pp. 294-306. https://doi.org/10.1016/j.biotechadv.2007.02.001
  5. Choi, H. J. and Lee, S. M. (2011). Effect of Temperature, Light Intensity and pH on the Growth Rate of Chlorella vulgaris, Journal of Korean Society of Environmental Engineers, 33(7), pp. 511-515. [Korean Literature] https://doi.org/10.4491/KSEE.2011.33.7.511
  6. Choi, H. J. and Lee, S. M. (2012). Effect of Photobioreactor with Optical Panel on the Growth Rate of Chlorella vulgaris, Journal of Korean Society of Environmental Engineers, 34(7), pp. 467-472. https://doi.org/10.4491/KSEE.2012.34.7.467
  7. Grobbelaar, J. U. (2000). Physiological and Technological Considerations for Optimizing Mass Algal Cultures, Journal of Applied Phycology, 12(3-5), pp. 201-206. https://doi.org/10.1023/A:1008155125844
  8. Haag, A. L (2007). Algae Bloom Again, Nature, 447, pp. 520-521. https://doi.org/10.1038/447520a
  9. Hsieh, C. H. and Wu, W. T. (2009). A Novel Photpbioreactor with Transparent Rectangular Chambers for Cultivation of Microalgae, Biochemical Engineering Journal, 46(3), pp. 300-305. https://doi.org/10.1016/j.bej.2009.06.004
  10. Hu, G., Kurano, N., Kawachi, M., Iwasaki, I., and Miyachi, S. (1998). Ultrahigh-cell-density Culture of a Marine Green Alga Chlorococcum littorale in a Flat-plate Photobioreactor, Applied Microbiology and Biotechnology, 49(6), pp. 655-662. https://doi.org/10.1007/s002530051228
  11. Javanmardian, M. and Palsson, B. O. (1991). High-density Photoautotrophic Algal Cultures: Design, Construction, and Operation of a Novel Photobioreactor System, Biotechnology and Bioengineering, 38(10), pp. 1182-1189. https://doi.org/10.1002/bit.260381010
  12. Jin, E., Polle, J. E. W., Lee, H. K., Hyun, S. M., and Chang, M. (2003). Xanthophylls in Microalgae: From Biosynthesis to Biotechnological Mass Production and Application, Journal of Microbiology and Biotechnology, 13(2), pp. 165-174.
  13. Lee, E. T. Y. and Bazin, M. J. (1990). A Laboratory Scale Air-lift Helical Photobioreactor to Increase Biomass Output Rate of Photosynthetic Algal Cultures, New Phytologist, 116(2), pp. 331-335. https://doi.org/10.1111/j.1469-8137.1990.tb04722.x
  14. Lee, K. Y. and Lee, C. G. (2001). Effect of Light/dark Cycles on Wastewater Treatments by Microalgae, Biotechnology and Bioprocess Engineering, 6(3), pp. 194-199. https://doi.org/10.1007/BF02932550
  15. Lee, Y. K. (2001). Micoalgal Mass Culture Systems and Methods: Their Limitation and Potential, Journal of Applied Phycology, 13(4), pp. 307-315. https://doi.org/10.1023/A:1017560006941
  16. Masojidek, J. and Torzillo, G. (2008). Mass Cultivation of Freshwater Microalgae, In Encyclopedia of Ecology, Academic Press, Oxford, UK, pp. 2226-2235.
  17. Mayo, A. W. and Noike, T. (1996). Effects of Temperature and pH on the Growth of Heterotrophic Bacteria in Waste Stabilization Pond, Water Research, 30(2), pp. 447-455. https://doi.org/10.1016/0043-1354(95)00150-6
  18. Moreno-Garrido, I. (2008). Microalgae Immobilization: Current Techniques and Uses, Bioresource Technology, 99(10), pp. 3949-3964. https://doi.org/10.1016/j.biortech.2007.05.040
  19. Ogbonna, J. C. and Tanaka, H. (2000). Light Requirement and Photosynthetic Cell Cultivation-development of Processes for Efficient Light Utilization in Photobioreactors, Journal of Applied Phycology, 12(3-5), pp. 207-218. https://doi.org/10.1023/A:1008194627239
  20. Richmond, A. and Cheng-Wu, Z. (2001). Optimization of a Flat Plate Glass Reactor for Mass Production of Nannochloropsis sp., Journal of Biotechnology, 85(3), pp. 259-269. https://doi.org/10.1016/S0168-1656(00)00353-9
  21. Sakai, N., Sakamoto, Y., Kishimoto, N., Chihara, M., and Karube, I. (1995). Chlorella Strains from Hot Springs Tolerant to High Temperature and High $CO_2$, Energy Conversion and Management, 36(6-9), pp. 693-696. https://doi.org/10.1016/0196-8904(95)00100-R
  22. Sierra, E. Acien, Fernandez, J. M. Garcia, Gonzalez, C., and Molina, E. (2008). Characterization of a Flat Plate Photobioreactor for the Production of Microalgae, Chemical Engineering Journal, 138(1-3), pp. 136-147. https://doi.org/10.1016/j.cej.2007.06.004
  23. Suh, I. S. and Lee, C. G. (2003). Photobioreactor Engineering; Design and Performance, Biotechnology and Bioprocess Engineering, 8(6), pp. 313-321. https://doi.org/10.1007/BF02949274
  24. Tadesse, I., Green, F. B., and Puhakka, J. A. (2004). Seasonal and Diurnal Variations of Temperatures, pH and Dissolved Oxygen in Advanced Integrated Wastewater Pond System Treating Tannery Effluent, Water Research, 38(3), pp. 645-654. https://doi.org/10.1016/j.watres.2003.10.006
  25. Torzillo, G., Carlozzi, P., Pushparaj, B., Montaini, E., and Materassi, R. (1993). A Two-plane Tubular Photobioreactor for Outdoor Culture of Spirulina, Biotechnology and Bioengineering, 42(7), pp. 891-898. https://doi.org/10.1002/bit.260420714
  26. Wu, Z. and Shi, X. (2007). Optimization for High-density Cultivation of Heterotrophic Chlorella based on a Hybrid Neural Network Model, Letter in Applied Microbiology, 44(1), pp. 13-18. https://doi.org/10.1111/j.1472-765X.2006.02038.x