Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
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Effects of Dental Mineral Trioxide Aggregate on Cellular Reactions Involved in Inflammation and Differentiation

염증 및 분화 관련 세포 반응에 대한 치과용 Mineral Trioxide Aggregate의 영향

  • Kim, Jong-Gil (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Son, Kyung Mi (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Park, Hee Chul (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Zhu, Tingting (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Kwon, Ji Hyun (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Yang, Hyeong-Cheol (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
  • 김종길 (서울대학교 치의학대학원 치과생체재료과학교실 및 치학연구소) ;
  • 손경미 (서울대학교 치의학대학원 치과생체재료과학교실 및 치학연구소) ;
  • 박희철 (서울대학교 치의학대학원 치과생체재료과학교실 및 치학연구소) ;
  • 주정정 (서울대학교 치의학대학원 치과생체재료과학교실 및 치학연구소) ;
  • 권지현 (서울대학교 치의학대학원 치과생체재료과학교실 및 치학연구소) ;
  • 양형철 (서울대학교 치의학대학원 치과생체재료과학교실 및 치학연구소)
  • Published : 2013.03.01
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Abstract

Mineral trioxide aggregate (MTA) is receiving more attentions in endodontic dental clinics due to its high sealing ability and biocompatibility. Although previous studies have reported the effects of MTA on dental pulp tissue, the mechanisms underlying on inflammation and differentiation at cellular levels are not yet fully understood. In this study, we investigated various molecular aspects of cellular reactions involved in inflammation and differentiation to commercially available MTAs including ProRoot MTA, Ortho MTA and MTA-Angelus. mRNA of COX-2 gene which is involved in inflammatory reaction was not increased by all tested MTAs, and expression of COX-2 by lipopolysaccharide was not severely altered by MTAs, demonstrating that COX-2 is not involved in inflammatory reactions to MTA. The extracts of all MTAs obtained for early 24 hrs increased ALP activities of human dental pulp (HDP) cells, while the extracts of ProRoot MTA and MTA-Angelus obtained for 1-3 and 3-6 day decreased ALP activity, indicating the presence of inhibitory components in the two MTAs. ProRoot MTA enhanced mRNA levels of DMP-1, but not DSPP. However, the other MTAs did not affect mRNA expression of DSPP, DMP-1, OCN and OPN, which is not consistent with ALP activities. Thus, further studies are needed to conclude the effects of MTA on differentiation of dental pulp cells.

Keywords

References

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