YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Immunoregulatory Effect of Ginsenoside Rd against $CD4^+$ Th lymphocyte

인삼배당체 Rd의 $CD4^+$ Th 임파구에 대한 면역조절효과

  • Joo, Inkyung (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Kim, Jeonghyeon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Shehzad, Omer (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Yeong Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Han, Yongmoon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University)
  • 주인경 (동덕여자대학교 약학대학 면역.미생물학교실) ;
  • 김정현 (동덕여자대학교 약학대학 면역.미생물학교실) ;
  • 오마 쉐자드 (서울대학교 약학대학 천연물연구소) ;
  • 김영식 (서울대학교 약학대학 천연물연구소) ;
  • 한용문 (동덕여자대학교 약학대학 면역.미생물학교실)
  • Received : 2013.01.04
  • Accepted : 2013.02.25
  • Published : 2013.02.28
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Abstract

In this present study, we determined the immunoregulatory activity of ginsenoside Rd extract from Panax ginseng. To determine the activity, we tested Rd against $CD4^+$ Th cells in a murine model of type 1 diabetes, which involves Th1-dominant immunity. The type 1 diabetes was caused by streptozotocin (STZ) and the severity of the diabetes was evaluated by measuring the degree of hyperglycemia, a major symptom of diabetes. The data resulting from experiments showed that ginsenoside Rd induced a greater level of Th1 type cytokines [IFN-${\gamma}$ & IL-2] than Th2 type [IL-4 & IL-10] (P<0.05), which was determined by cytokine profile analysis. In the animal model of diabetes, the depletion of $CD4^+$ Th cells by a treatment of anti-CD4 mAb resulted in considerably lower values of blood-glucose levels than those of the mAb-untreated mice, which indicates that the Th1 immune response from $CD4^+$ Th cells are responsible for diabetes. Based on these observations, the effect of Rd on diabetes was examined in the same animal model. Results showed that Rd-treated mice groups had increased levels of blood glucose compared to Rd-untreated mice groups that were used as a negative control (P<0.05). In other words, Rd aggravated the diabetes via the Th1 immune response. In conclusion, ginsenoside Rd had an immunoregulatory activity of Th1-dominant immunity.

Keywords

References

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