Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Genetic Diversity and Structure of the Korean Endemic Species, Coreanomecon hylomeconoides Nakai, as Revealed by ISSR markers

한국 특산식물 매미꽃(Coreanomecon hylomeconoides Nakai) 집단의 유전다양성 및 구조

  • Received : 2013.03.04
  • Accepted : 2013.04.25
  • Published : 2013.04.30
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Abstract

The genetic diversity and structure of eight populations of Coreanomecon hylomeconoides Nakai, an endemic Korean plant, were investigated using 50 ISSR loci from eight primers. The average percentage of polymorphic loci was 47.3%. The Shannon's index (SI=0.218) and gene diversity (h=0.142) were relatively lower than those of other long-lived perennials. The Sancheong (SI=0.233, h=0153), Gwangyang (SI=0.263, h=0.171), and Suncheon (SI=0.241, h=0.159) populations showed greater genetic diversity than the Namhae and Gwangju populations, which are on the edge of the distribution. Analysis of molecular variance (AMOVA) showed that 18% of the total variation could be attributed to differences among populations, and 82% to differences within populations, indicating moderate gene flow among adjacent populations. These results were supported by value of Nm (2.184). The UPGMA conducted using the genetic distance and Bayesian cluster analysis showed a remarkable geographic trend structured into east and west regions. Overall, the results indicate that the Sancheong and Gwangyang populations, which had a large population size and higher degree of genetic diversity, should be the focus of in situ conservation.

우리나라 특산식물인 매미꽃(Coreanomecon hylomeconoides Nakai) 집단의 유전적 다양성 및 구조를 조사하기 위해 8집단 224개체에 대한 ISSR(Inter Simple Sequence Repeat) 분석이 수행되었다. 총 8개의 ISSR 프라이머를 이용하여 50개의 증폭산물을 관찰하였으며 집단 수준에서의 유전적 다양성의 평균은 P (Percentage of polymorphic loci) = 47.3%, SI(Shannon's information index) = 0.218, h (Nei's genetic diversity) = 0.142로 다년생 초본류의 평균보다는 월등히 낮게 나타났다. 집단별로는 분포의 중심에 해당하는 산청(SI=0.233, h=0153), 광양(SI=0.263, h=0.171), 순천(SI=0.241, h=0.159) 집단이 남해(SI=0.183, h=0.116)나 광주(SI=0.181, h=0.121)의 변두리 집단보다는 비교적 높은 유전다양성을 유지하는 것으로 나타났다. AMOVA 분석 결과 전체 유전변이의 약 18%가 지역 간에 나머지 82%가 집단 내 개체간의 차이에 기인하는 것으로 나타났는데 이는 집단 간에 유전자 교류가 원활히 이루어지기 때문으로 판단된다. 유전적 거리를 이용한 UMGMA 유집분석과 Bayesian cluster 분석 결과, 매미꽃 집단은 동서 두 지역으로 구조화 되는 경향을 보여 주었는데 이는 집단의 지리적 분포 패턴의 영향인 것으로 추정할 수 있다. 본 연구 결과, 조사된 다른 집단보다 풍부한 개체수와 높은 유전 다양성을 유지하고 있는 지리산 및 백운산의 산청, 광양 집단들에 대한 적극적인 현지 내(in situ) 보전대책 수립이 요구된다.

Keywords

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