Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
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Effect of Inflammatory Responses to PLGA Films Incorporated Hesperidin: In vitro and In vivo Results

PLGA/헤스페리딘 함량별 필름에서 염증 완화 효과: In vitro, In vivo 결과

  • Song, Jeong Eun (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University) ;
  • Shim, Cho Rok (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University) ;
  • Lee, Yujung (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University) ;
  • Ko, Hyun Ah (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University) ;
  • Yoon, Hyeon (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University) ;
  • Lee, Dongwon (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University) ;
  • Khang, Gilson (Dept. of BIN Fusion Tech, and Dept. of PolymerNano Sci. & Tech., Chonbuk National University)
  • 송정은 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 심초록 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 이유정 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 고현아 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 윤현 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 이동원 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 강길선 (전북대학교 BIN 융합공학과, 고분자나노공학과)
  • Received : 2012.10.22
  • Accepted : 2013.01.06
  • Published : 2013.05.25
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Abstract

Hesperidin (Hes) has known to having some functions like protection of blood circulatory system, anti-tumor effect, antioxidant effect and anti-inflammatory effect. The goal of this study is to demonstrate the relationship between Hes and inflammatory through in vitro and in vivo studies using poly(lactic-co-glycolic acid) (PLGA) film including Hes as a tissue engineered scaffold. To confirm the proliferation of cells on fabricated scaffold, cells (RAW 264.7 and NIH/3T3) were seeded on PLGA/Hes film then analyzed with MTT and SEM at 1 and 3 days after seeding. The results from ELISA, RT-PCR, and FACS for anti-oxident and anti-inflammatory effect showed that inflammatory response of PLGA/Hes film decreased more than that of PLGA film. Also, in vivo result confirmed that inflammatory response by implanted PLGA/Hes film decreased more comparing with PLGA film. This is because of anti-inflammatory effect of Hes reducing induced inflammatory cell and accumulation of fibrous capsule. The results showed that PLGA/Hes film's capacity on reducing inflammatory is better than PLGA film because of Hes.

헤스페리딘은 혈관 순환계 보호 효과, 항암 효과, 항산화 효과, 항염증 효과 등의 작용이 있다고 알려져 있다. 본 연구에서는 헤스페리딘을 함유한 poly(lactic-co-glycolic acid)(PLGA) 필름을 조직공학적 세포담체로 이용하고 생체 내/외의 실험을 통하여 헤스페리딘과 염증 반응의 상관관계를 알아보고자 하였다. 세포담체에서의 세포의 증식과 부착을 확인하고자 PLGA/헤스페리딘 필름에 세포(RAW 264.7, NIH/3T3)를 파종하여 1, 3일 후에 MTT와 SEM을 통하여 분석하였다. 또한 항산화 효과와 염증 완화 효과를 알아보고자 ELISA, RT-PCR, FACS 등을 평가한 결과 PLGA 필름보다는 헤스페리딘이 첨가된 PLGA 필름에서 염증 반응이 감소하는 것을 확인하였다. In vivo 실험에서도 헤스페리딘이 PLGA에 의해 유도된 염증세포와 섬유피막 축적을 감소시켜 헤스페리딘의 항염증 효과를 볼 수 있었다. 이 실험 결과 PLGA 필름보다는 PLGA/헤스페리딘 필름에서 염증 반응이 감소함을 확인하였다.

Keywords

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