한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제42권4호
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  • Pages.556-562
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  • 2013
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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톳 유래 수용성 다당류의 Lipase 저해활성과 지질 및 체중변화에 미치는 영향

Effects of Water-Soluble Polysaccharides from Tott on Lipid Absorption and Animal Body Weight

  • 백구 (가천대학교 약과학과) ;
  • 구본근 (가천대학교 약과학과) ;
  • 안병제 (전남해양바이오산업센터) ;
  • 박제권 (가천대학교 생명과학과)
  • 투고 : 2013.01.04
  • 심사 : 2013.02.07
  • 발행 : 2013.04.30
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초록

본 연구에서는 기존의 유기용매를 이용한 추출을 지양하고 환경 친화적이고 간단한 추출방법을 고안하여 톳의 다당류 성분(WSP-A)을 추출해내었고, WSP-A가 지질의 흡수에 미치는 효과를 알아보기 위하여 in vitro 상으로 lipase inhibition assay와 in vivo로 고지방 식이로 비만을 유도한 rat을 이용한 동물실험을 수행하였다. 그 결과 톳 유래 다당류는 alginate와 유사한 성분이지만 성분당 분석을 통하여 fucose가 주 당성분인 polysaccharide로 밝혀졌고, 이는 alginate보다 뛰어나고 안정한 lipase inhibitor로써 작용하였다. 또한 동물실험에서는 WSP-A를 포함하는 음용수로 제공한 것과 제공하지 않은 대조군은 무게 변화량에 유의적인 차이를 보일 뿐 아니라 비만 예방군과 비만 치료군 사이에서도 유의적인 차이를 나타내었다. 식이섬유를 이용한 연구와 비교해보았을 때, 톳 유래 다당류는 식이섬유 이상의 효과뿐만 아니라 뛰어난 lipase inhibitor로 체중감량을 나타내었고 이는 인간췌장유래 리파아제(human pancreatic lipase)의 저해활성과 더불어 인체 내에서의 작용 가능성을 짐작하고 있다. 따라서 톳 유래 다당류 성분은 식이성 섬유의 공급원 뿐만 아니라 추후에 효소활성 저해 효과에 대한 보다 명확한 작용기전을 밝혀낸다면 단순한 건강 기능성 식품 소재보다 lipase inhibitor로써 항비만 약물로 고부가가치를 창출해낼 수 있을 것으로 사료된다.

We examined the anti-obesity effects of water-soluble polysaccharides (WSP-A) extracted from the seaweed Hijikia fusiforme (Tott in Korean). The extracted alginate-like polysaccharide (verified by FT-IR and HPAEC-PAD analysis) was examined in a lipase inhibition assay and animal experiments. WSP-A inhibited lipase up to 30%, with over 80% of the initial activity retained until the 1 hour reaction in vitro. There was a 30% loss in the rate of weight gain in rats fed a high-fat diet. WSP-A therefore seems to serve as a healthy weight loss agent by inhibiting lipases, thus preventing the absorption of fat in the body.

키워드

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  1. Quality characteristics of Hijikia fusiforme extracts with different extraction method vol.22, pp.1, 2015, https://doi.org/10.11002/kjfp.2015.22.1.70
  2. In Vitro Screening for Anti-Dementia Activities of Seaweed Extracts vol.45, pp.7, 2016, https://doi.org/10.3746/jkfn.2016.45.7.966
  3. Processing and Quality Characteristics of Glutinous Barley Gruel Containing Hizikia fusiformis vol.49, pp.3, 2016, https://doi.org/10.5657/KFAS.2016.0310
  4. 추출방법에 따른 톳 추출물의 항산화 및 생리활성 특성 vol.24, pp.5, 2013, https://doi.org/10.11002/kjfp.2017.24.5.631
  5. Inhibitory Effects of Crude Fucoidan Extract from Hizikia fusiformis against Norovirus Causing Foodborne Disease vol.34, pp.5, 2018, https://doi.org/10.9724/kfcs.2018.34.5.519
  6. 소금 첨가에 따른 도라지 발효 특성과 미생물 변화 및 항비만 효능 평가 vol.18, pp.2, 2018, https://doi.org/10.15429/jkomor.2018.18.2.64