Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Detection of PCB77 by Indirect Competitive Enzyme-linked Immunosorbent Assay in Sea Sediment Samples

  • Chen, Han-Yu (School of Environmental Science and Engineering, Shanghai Jiao Tong University) ;
  • Zhuang, Hui-Sheng (School of Environmental Science and Engineering, Shanghai Jiao Tong University) ;
  • Yang, Guang-Xin (School of Environmental Science and Engineering, Shanghai Jiao Tong University)
  • Received : 2012.10.31
  • Accepted : 2012.12.27
  • Published : 2013.03.20
Download PDF
⟨ Previous Next ⟩

Abstract

3,3',4,4'-Tetrachlorobiphenyl (IUPAC PCB77) is one of seven indicative polychlorinated biphenyls (PCBs) in the surface sediments. The current study presents a novel polyclonal antibody for the determination of the PCB77 using indirect competitive enzyme-linked immunosorbent assay. Under optimum conditions, PCB77 was determined within the concentration range of 0.01-100 ${\mu}g\;L^{-1}$, with a detection limit of 0.057 ${\mu}g\;L^{-1}$. The assays were tested for their cross-reactivity profiles using 3 selected congeners and 4 Aroclor products. The assays were highly specific for coplanar PCB congeners, but less specific for Aroclor1248. The spiked recoveries from five sediment samples were 86%-114% for PCB77 from ELISA, which were satisfactory. The current study demonstrated that the developed antiserum and immunoassay procedure can be used to detect PCB77 in environmental samples. The results of the sediment analysis were confirmed by conventional GC/ECD.

Keywords

References

  1. Kannan, K.; Kajiwara, N., Le Boeuf, B. J.; Tanabe, S. Environ. Pollu. 2004, 131, 425. https://doi.org/10.1016/j.envpol.2004.03.004
  2. Li, S.; Frank, W.; Ying, D. L.; Camilla, T. Environ. Pollu. 2006, 144, 434. https://doi.org/10.1016/j.envpol.2005.12.054
  3. Bonefeld-Jorgensen, E. C.; Andersen, H. R.; Rasmussen, T. H. Toxic. 2001, 158, 141. https://doi.org/10.1016/S0300-483X(00)00368-1
  4. Gilbert, R. Ecotoxicology and Environ. Safe. 2004, 59, 275. https://doi.org/10.1016/j.ecoenv.2004.06.003
  5. Sager, D. B.; Shih-Schroeder, W.; Girard, D. Bull. Environ. Contam. Toxicol. 1987, 38, 946. https://doi.org/10.1007/BF01609078
  6. Durand, B.; Dufour, B., Fraisse, D. Chemosphere 2008, 70, 689. https://doi.org/10.1016/j.chemosphere.2007.06.057
  7. Yang, F. X.; Wilcox, B.; Jin, S. W.; Aguirre, A. A. Chemosphere 2008, 73, 133. https://doi.org/10.1016/j.chemosphere.2008.03.066
  8. Cachada, A.; Lopes, L. V.; Hursthouse, A. S.; Biasioli, M.; Gr man, H. Environ. Pollu. 2009, 157, 511. https://doi.org/10.1016/j.envpol.2008.09.002
  9. Cao, L. L.; Yan, C. H.; Yu, X. D.; Tian, Y.; Zhao, L.; Liu, J. X.; Shen, X. M. Sci. Total Environ. 2001, 409, 2997.
  10. Jerry, W. A. J. High Resolution Chromatography 1991, 16, 369.
  11. Na, Y. C.; Kim, K. J.; Hong, J. Chemosphere 2008, 73, S7. https://doi.org/10.1016/j.chemosphere.2006.12.102
  12. Kotaro, M.; Nobutoshi, O.; Kiyoshi, N. Anal. Sci. 2011, 27, 421. https://doi.org/10.2116/analsci.27.421
  13. Ayato, K.; Takashi, M.; Katsuhisa, H. Anal. Sci. 2006, 22, 1393. https://doi.org/10.2116/analsci.22.1393
  14. Tomoaki, T.; Yoshiaki, A.; Akira, O. Chemosphere 2006, 65(3), 467. https://doi.org/10.1016/j.chemosphere.2006.01.059
  15. Chiu, Y. W.; Li, Q. X.; Karu, A. E. Anal. Chem. 2001, 73, 5477. https://doi.org/10.1021/ac0102462
  16. Shimomura, M.; Nomura, Y.; Zhang, W. Anal. Chim. Acta 2001, 434, 223. https://doi.org/10.1016/S0003-2670(01)00809-1
  17. Javier, C. J.; Catherine, G. J. Environ. Monit. 2011, 13, 894. https://doi.org/10.1039/c0em00569j
  18. Glass, T. R.; Ohmura, N.; Saiki, H.; Sawadaishi, K. Anal. Chim. Acta 2004, 517, 161. https://doi.org/10.1016/j.aca.2004.04.038
  19. Carlo, M. D.; Mascini, M. Analy. Chim. Acta 1996, 336, 167. https://doi.org/10.1016/S0003-2670(96)00377-7
  20. Franek, M.; Deng, A. P.; Kola, V. Anal. Chim. Acta 2001, 444, 131. https://doi.org/10.1016/S0003-2670(01)01162-X
  21. Chuang, C. J.; Miller, L. S.; Davis, D. B. Anal. Chim. Acta 1998, 376, 67. https://doi.org/10.1016/S0003-2670(98)00439-5
  22. Kania-Korwel, I.; Parkin, S.; Robertson, L. W. Chemosphere 2004, 56, 735. https://doi.org/10.1016/j.chemosphere.2004.04.035
  23. Nuria, P. N.; Sergi, M.; Angel, M.; Rosa, P. Anal. Chim. Acta 2007, 594, 211. https://doi.org/10.1016/j.aca.2007.05.045
  24. Zhang, M. C.; Wang, Q. E.; Zhuang, H. S. Anal Bioanal Chem. 2006, 386, 1401. https://doi.org/10.1007/s00216-006-0703-z
  25. Aydin, M. E.; Tor, A.; Ozcan, S. Anal. Chim. Acta 2006, 577, 32. https://doi.org/10.1016/j.aca.2006.05.101

Cited by

  1. A sensitive immunosorbent bio-barcode assay based on real-time immuno-PCR for detecting 3,4,3',4'-tetrachlorobiphenyl vol.406, pp.6, 2014, https://doi.org/10.1007/s00216-013-7583-9
  2. An indirect competitive enzyme-linked immunosorbent assay for the determination of 3,4-dichlorobiphenyl in sediment using a specific polyclonal antibody vol.6, pp.3, 2014, https://doi.org/10.1039/C3AY41993B