Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Identification of water mold from wild brook lamprey, Lethenterone reissneri

자연산 다묵장어, Lethenterone reissneri에서 발생한 물곰팡이병 원인체의 동정

  • Kim, Hyoung Jun (National Fishery Products Quality Management Services, Incheon Regional Office) ;
  • Park, Jeong Su (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Kim, Sung Yeon (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Koo, Ja Geun (Incheon Fisheries Research Institute) ;
  • Bang, In-Chul (Department of Life Science and Biotechnology, Soonchunhyang University) ;
  • Kwon, Se Ryun (Department of Aquatic Life Medical Sciences, Sunmoon University)
  • 김형준 (국립수산물품질관리원 인천지원) ;
  • 박정수 (선문대학교 수산생명의학과) ;
  • 김성연 (선문대학교 수산생명의학과) ;
  • 구자근 (인천수산자원연구소) ;
  • 방인철 (순천향대학교 생명시스템학과) ;
  • 권세련 (선문대학교 수산생명의학과)
  • Received : 2013.01.30
  • Accepted : 2013.04.02
  • Published : 2013.04.30
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Abstract

Saprolegnia isolate from wild brook lamprey was identified on the basis of its morphological and molecular characteristics. The isolates showed aseptic hyphae and clavate zoosporagium. Zoospores discharge was typically saprolegnoid. Neither oogomia nor antheridia was observed in this study. ITS sequence obtained from the isolate was compared with other Saprolegnia spp. to analyse their phylogenetic relationships. Results showed that the isolate belongs to clade I including Saprolegnia parasitica. Based on the asexual organs, zoospore discharge manner and ITS sequence analysis, the isolate was identified as S. parasitica.

자연산 다묵장어, Lethenterone reissneri에서 발생한 물곰팡이병의 원인체를 형태학적 및 유전학적 연구를 통하여 동정하였다. 분리균주는 격벽이 없는 균사를 형성하였고, 가늘고 긴 모양의 유주자낭 내에 활발한 운동성을 보이는 유주자가 여러 줄로 배열되었다가 유주자낭의 선단에서 휴면하지 않고 한꺼번에 방출되는 특징을 보였다. 한편 유성생식기관인 조란기와 조정기는 관찰되지 않았다. 또한 분리균주의 ITS sequence를 분석하고 Saprolegnia 속의 다른 분리균주와 유전학적 상관관계를 조사한 결과, S. parasitica가 속해있는 clade I에 분류되는 것을 확인할 수 있었다. 무성생식기관의 형태, 유주자 방출방식 및 ITS sequence의 phylogenetic analysis에 근거하여 자연산 다묵장어에서 관찰된 물곰팡이병의 원인체는 S. parasitica인 것으로 동정되었다.

Keywords

References

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