한국어병학회지 (Journal of fish pathology)

  • 제26권1호
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  • Pages.31-38
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  • 2013
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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멍게, Halocynthia roretzi 물렁증의 원인충인 Azumiobodo hoyamushi의 살충효과 평가를 위한 현미경계수법과 alamar blue assay 비교

Comparison of microscopic counting and alamar blue assay to evaluate anti-protozoal effects against Azumiobodo hoyamushi that causes soft tunic syndrome to Halocynthia roretzi

  • 이재근 (군산대학교 해양과학대학 수산생명의학과) ;
  • 전승렬 (군산대학교 해양과학대학 수산생명의학과) ;
  • 박경일 (군산대학교 해양과학대학 수산생명의학과) ;
  • 최상훈 (군산대학교 해양과학대학 수산생명의학과) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과)
  • Lee, Jae-Geun (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Zeon, Seung-Ryul (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Kyung-Il (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, Kunsan National University)
  • 투고 : 2012.11.05
  • 심사 : 2013.04.02
  • 발행 : 2013.04.30
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초록

The edible ascidian, Halocynthia roretzi is a commercially important fisheries resource in Korea. However, there have been outbreaks of mass mortality due to soft tunic syndrome. It was discovered recently that the cause of death is infection by a protozoan parasite Azumiobodo hoyamushi. Alamar blue assay and microscopic counting were used to estimate anti-protozoal effects of 20 drugs having different action mechanisms. Through comparison of alamar blue assay and microscopic counting, 6 drugs were found to be potential in protozoan-killing effects: amphotericin B, formalin, hydrogen peroxide, bithionol, benzalkonium chloride, bronopol (24hr-$EC_{50}{\leq}20{\mu}g/ml$). The preliminary data can be used as a basis to develop anti-protozoal agents against A. hoyamushi.

키워드

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피인용 문헌

  1. Methods to eradicate soft tunic syndrome (STS)-causing protozoa Azumiobodo hoyamushi, the highly infectious parasite from the edible ascidian (Halocynthia roretzi) vol.19, pp.1, 2016, https://doi.org/10.1186/s41240-016-0002-3
  2. The kinetoplastid parasite Azumiobodo hoyamushi, the causative agent of soft tunic syndrome of the sea squirt Halocynthia roretzi, resides in the East Sea of Korea vol.116, 2014, https://doi.org/10.1016/j.jip.2013.12.006