Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Identification of Differentially Expressed Genes by Gabapentin in Cultured Dorsal Root Ganglion in a Rat Neuropathic Pain Model

  • Heo, Ji Hye (Department of Biomedical Science, Korea University) ;
  • Lee, Seung Ha (Department of Environmental Health, Korea University) ;
  • Chang, Kyung Ha (Department of Biomedical Science, Korea University) ;
  • Han, Eun Hye (Department of Biomedical Science, Korea University) ;
  • Lee, Seung Gwan (Department of Biomedical Science, Korea University) ;
  • Choi, Dal Woong (Department of Environmental Health, Korea University) ;
  • Kim, Suhng Wook (Department of Biomedical Science, Korea University)
  • Received : 2013.02.06
  • Accepted : 2013.03.12
  • Published : 2013.03.31
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Abstract

Neuropathic pain is a chronic pain disorder caused by nervous system lesions as a direct consequence of a lesion or by disease of the portions of the nervous system that normally signal pain. The spinal nerve ligation (SNL) model in rats that reflect some components of clinical pain have played a crucial role in the understanding of neuropathic pain. To investigate the direct effects of gabapentin on differential gene expression in cultured dorsal root ganglion (DRG) cells of SNL model rats, we performed a differential display reverse transcription-polymerase chain reaction analysis with random priming approach using annealing control primer. Genes encoding metallothionein 1a, transforming growth factor-${\beta}1$ and palmitoyl-protein thioesterase-2 were up-regulated in gabapentin-treated DRG cells of SNL model rats. The functional roles of these differentially expressed genes were previously suggested as neuroprotective genes. Further study of these genes is expected to reveal potential targets of gabapentin.

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References

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