The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Condition for Mass Production of Antagonistic Bacterium Burkholderia pyrrocinia CAB08106-4 to Control Garlic White Rot

마늘 흑색썩음균핵병 방제 길항세균 Burkholderia pyrrocinia CAB08106-4의 대량배양 조건

  • Lee, Dong Guk (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.) ;
  • Lee, Eun Sook (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.) ;
  • Kim, Jeong Seok (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.) ;
  • Baek, Cheol Ki (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.) ;
  • Park, Mae Sol (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.) ;
  • Park, Eun Hee (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.) ;
  • Lee, Suk Hee (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.) ;
  • Chung, Chang Kook (Agricultural Research Center, HANKOOKSAMGONG Co. Ltd.)
  • Received : 2013.01.08
  • Accepted : 2013.03.08
  • Published : 2013.03.31
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Abstract

Burkholderia pyrrocinia CAB08106-4 was parceled out from the Chungnam Agricultural Research and Extension Center, Korea to evaluate the antagonistic activity against garlic white rot caused by Sclerotium cepivorum. The optimum cultural conditions including temperature, pH, enzyme activity, carbon and nitrogen sources were determined. The optimum culture conditions of B. pyrrocinia CAB08106-4 were $286^{\circ}C$, 150 rpm and pH 7. Chitinase only showed activity among several tested enzymes. The highest cell growth was obtained with 1% glucose and 0.1% $(NH_4)_2SO_4$, respectively.

길항균주의 기초배양 조건 및 특성은 nutrient broth 배지를 사용하여 조사하였고, 그 결과 온도는 $286^{\circ}C$, pH는 6이었으며 효소활성은 chitinase로 관찰이 되었다. 대량배양 조건은 pseudomonas medium을 이용하였고 위와 동일한 기초 배양조건들을 사용하였으며, 항균활성과 생육정도를 동시에 충족시키는 탄소원과 질소원을 조사하였다. 그 결과 탄소원 13종 중에서 glucose, 질소원은 8종류 중에서 $(NH_4)_2SO_4$로 나타났다. 또한 각각의 탄소원과 질소원의 농도는 1%, 0.1%에서 가장 좋은 것으로 나타났다.

Keywords

References

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