생명과학회지 (Journal of Life Science)

  • 제23권3호
  • /
  • Pages.341-346
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해조류 무절산호조 혹돌잎의 생물학적 특성 및 조직구조

Biological Characteristics and Tissue Structure of a Crustose Coralline Lithophyllum Alga

  • 강지영 (부경대학교 생물공학과) ;
  • ;
  • 이익준 (부경대학교 생물공학과) ;
  • 최지영 (부경대학교 생물공학과) ;
  • 주진 (경북대학교 응용화학과) ;
  • 최유성 (충남대학교 화학공학과) ;
  • 황동수 (포항 공과대학교 해양대학원) ;
  • 홍용기 (부경대학교 생물공학과)
  • Kang, Ji-Young (Department of Biotechnology, Pukyong National University) ;
  • Benliro, Ianthe Marie P. (KOICA-PKNU International Graduate Program of Fisheries Science, Pukyong National University) ;
  • Lee, Ik-Joon (Department of Biotechnology, Pukyong National University) ;
  • Choi, Ji-Young (Department of Biotechnology, Pukyong National University) ;
  • Joo, Jin (Department of Applied Chemistry, Kyungpook National University) ;
  • Choi, Yoo Seong (Department of Chemical Engineering, Chungnam National University) ;
  • Hwang, Dong Soo (Postech Ocean Science & Technology Institute, Pohang University of Science & Technology) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
  • 투고 : 2013.01.24
  • 심사 : 2013.03.11
  • 발행 : 2013.03.30
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초록

연안 암반지역에서 해조류 군락의 소실 즉 백화 혹은 갯녹음현상은 산호조류와 관련성이 있다. 대표적인 무절산호조의 생물학적 특성을 파악하기 위하여 18S rDNA 유전자를 분석한 결과 혹돌잎(Lithophyllum) 속에 속하는 것을 확인하였고 그 형태적 특성으로 보아 L. yessoense 종인 것으로 유추된다. Triphenyl tetrazolium chloride로서 활력을 측정한 결과 12월에서 2월 사이가 가장 높았으며, 조직 활력을 유지하기 위하여는 $16^{\circ}C$, 16:8 시간 명암 광주기, 30 ${\mu}E/m^2/s$ 광도에서 5일간 최적상태를 보였다. 지방산 조성에서는 EPA가 가장 많은 고도불포화지방산으로서 9.7%를 차지하고 있다. 주사형전자현미경에 의한 표면구조를 보면 평균 3.6 ${\mu}m$ 직경의 둥근 함몰 분화구 모양을 이루며 그 위에 1.0 내지 3.7 ${\mu}m$의 비정형 다각형 구조물들이 덮여져 있다. 이 같은 조성과 구조를 바탕으로 한 생체모방 산호조는 해조류 등에 대한 환경친화적 방오소재로서 활용되어질 수도 있을 것이다.

The disappearance of seaweed flora in some rocky areas, which is known as algal whitening, barren ground, coralline flats, or deforested areas, is associated with some species of coralline algae. To determine the biological characteristics of a representative species of crustose coralline alga, the 18S rDNA gene was sequenced to identify the genus Lithophyllum. According to its morphological and distributional characteristics, it was deduced to be L. yessoense. Viability was measured using triphenyl tetrazolium chloride and showed high viability from December to February. Culture conditions of $16^{\circ}C$, a 16 hr light, 8 hr dark cycle, and 30 ${\mu}E/m^2/s$ light intensity were optimal for maintaining the viability of the alga for up to five days. Included in the fatty acids was 9.7% ${\omega}$-3 eicosapentaenoic acid. An electron microscopy scan of the surface structure revealed round craters about 3.6 ${\mu}m$ in diameter, which were covered with rough, irregular, and angular polygon-shaped structures about 1.0 to 3.7 ${\mu}m$ in size. Based on the composition and structure found in our study, biomimetic coralline alga might become an environmentally friendly antifouling material against the attachment of soft foulants.

키워드

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