Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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An Autochthonous Case of Canine Visceral Leishmaniasis in Korea

  • Bhang, Dong Ha (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Choi, Ul Soo (Department of Veterinary Clinical Pathology and Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Hyun Jeong (College of Veterinary Medicine, Chonnam National University) ;
  • Cho, Kyoung-Oh (College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Sung-Shik (College of Veterinary Medicine, Chonnam National University) ;
  • Youn, Hee-Jeong (Veterinary Parasitology, College of Veterinary Medicine, Seoul National University) ;
  • Hwang, Cheol-Yong (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Youn, Hwa-Young (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University)
  • Received : 2013.05.22
  • Accepted : 2013.08.18
  • Published : 2013.10.31
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Abstract

A 12-year-old spayed female mixed-bred dog presented with nasal bleeding of 2 days duration and a skin nodule in the left flank. No abnormalities were found in coagulation profiles and blood pressure. Cytological evaluation of the nodule revealed numerous characteristic round organisms having a nucleus and a bar within macrophages and in the background, consistent with leishmaniasis. In vitro culture was unsuccessful but PCR of the nodular aspirate identified the organisms as Leishmania infantum, and the final diagnosis was canine leishmaniasis. No history of travel to endemic countries was noted. Because the dog had received a blood transfusion 2 years before the illness, serological screening tests were performed in all donor dogs of the commercial blood bank using the commercial Leishmania ELISA test kit, and there were no positive results. Additional 113 dogs with hyperglobulinemia from Seoul were also screened with the same kits but no positive results were obtained. To the best of the author's knowledge this is the first autochthonous case of canine leishmaniasis in Korea.

Keywords

References

  1. Baneth G. Leishmaniasis. In Greene CE ed, Infectious Disease of the dog and cat. St. Louis, USA. Saunders. 2006, p 685-695.
  2. Heu IM. Three cases of Kala-azar, especially on the various serologic reaction. Korean J Int Med 1952; 1: 118-121.
  3. Kim YJ, Hwang ES, You DS, Son SW, Uhm CS, Kim IH. A case of localized cutaneous leishmaniasis in a native Korean. Korean J Dermatol 2004; 42: 884-888.
  4. Ahn MH. Imported parasitic diseases in Korea. Infect Chemother 2010; 42: 271-279. https://doi.org/10.3947/ic.2010.42.5.271
  5. Shin JY, Lee YB, Cho BK, Park HJ. New world cutaneous leishmaniasis treated with intralesional injection of pentavalent antimony. Ann Dermatol 2013; 25: 80-83. https://doi.org/10.5021/ad.2013.25.1.80
  6. Merlen T, Sereno D, Brajon N, Rostand F, Lemesre JL. Leishmania spp.: completely defined medium without serum and macromolecules (CDM/LP) for the continuous in vitro cultivation of infective promastigote forms. Am J Trop Med Hyg 1999; 60: 41-50. https://doi.org/10.4269/ajtmh.1999.60.41
  7. Francino O, Altet L, Sánchez-Roberta E, Rodriguezc A, Solano- Gallegoc L, Alberolac J, Ferrerd L, Sáncheza A, Rourab X. Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniasis. Vet Parasitol 2006; 137: 214-221. https://doi.org/10.1016/j.vetpar.2006.01.011
  8. Van Eys G J, Schoone GJ, Kroon NC, Ebeling SB. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol Biochem Parasitol 1992; 51: 133-142. https://doi.org/10.1016/0166-6851(92)90208-2
  9. da Silva ES, van der Meide WF, Schoone GY, Gontijo CM, Schallig HD, Brazil RP. Diagnosis of canine leishmaniasis in the endemic area of Belo Horizonte, Minas Gerais, Brazil by parasite, antibody and DNA detection assays. Vet Res Commun 2006; 30: 637-643. https://doi.org/10.1007/s11259-006-3324-2
  10. Roura X, Sanchez A, Ferrer A. Diagnosis of canine leishmaniasis using a PCR technique. Vet Rec 1999; 144: 262-264. https://doi.org/10.1136/vr.144.10.262
  11. Ashford DA, Bozza M, Freire M, Miranda JC, Sherlock I, Eulalio C, Lopes U, Fernandes O, Degrave W, Barker RH Jr. Comparison of the polymerase chain reaction and serology for the detection of canine visceral leishmaniasis. Am J Trop Med Hyg 1995; 50: 251-255.
  12. Mathis A, DePlazes P. PCR and in vitro cultivation for detection of Leishmania spp. in diagnostic samples from humans and dogs. J Clin Microbiol 1995; 33: 1145-1149.
  13. Tánczos B, Balogh N, Király L, Biksi I, Szeredi L, Gyurkovsky M, Scalone A, Fiorentino E, Gramiccia M, Farkas R. First record of autochthonous canine leishmaniasis in Hungary. Vector Borne Zoonotic Dis 2012; 12: 588-594. https://doi.org/10.1089/vbz.2011.0906
  14. Pagliano P, Carannante N, Rossi M, Gramiccia M, Faella FS, Gaeta GB. Visceral leishmaniasis in pregnancy: a case series and a systematic review of the literature. J Antimicrob Chemother 2005; 55: 299-233.
  15. Rosypal AC, Troy GC, Zajac AM, Frank G, Lindsay DS. Transplacental transmission of a North American isolate of Leishmania infantum in an experimentally infected beagle. J Parasitol 2005; 92: 970-972.
  16. de Freitas E, Melo MN, da Costa-Val, AP, Michalick MS. Transmission of Leishmania infantum via blood transfusion in dogs: potential for infection and importance of clinical factors. Vet Parasitol 2006; 137: 159-167. https://doi.org/10.1016/j.vetpar.2005.12.011
  17. Kubar J, Quaranta JF, Marty P, Lelièvre A, Fichoux YL, Aufeuvre JP. Transmission of Leishmania infantum by blood donors. Nat Med 1997; 3: 368. https://doi.org/10.1038/nm0497-368

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