Parasites, Hosts and Diseases

  • 제51권4호
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  • Pages.421-426
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  • 2013
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Influence of Toxoplasma gondii Acute Infection on Cholinesterase Activities of Wistar Rats

  • 투고 : 2013.03.15
  • 심사 : 2013.06.05
  • 발행 : 2013.08.31
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초록

Several studies have shown the mechanisms and importance of immune responses against Toxoplasma gondii infection and the notable role of cholinesterases in inflammatory reactions. However, the association between those factors has not yet been investigated. Therefore, the aim of this study was to evaluate the acetylcholinesterase (AChE) activity in blood and lymphocytes and the activity of butyrylcholinesterase (BChE) in serum of rats experimentally infected with T. gondii during the acute phase of infection. For that, an in vivo study was performed with evaluations of AChE and BChE activities on days 5 and 10 post-infection (PI). The activity of AChE in blood was increased on day 5 PI, while in lymphocytes its activity was enhanced on days 5 and 10 PI (P<0.05). No significant difference was observed between groups regarding to the activity of BChE in serum. A positive (P<0.01) correlation was observed between AChE activity and number of lymphocytes. The role of AChE as an inflammatory marker is well known in different pathologies; thus, our results lead to the hypothesis that AChE has an important role in modulation of early immune responses against T. gondii infection.

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참고문헌

  1. Dubey JP. The history and life cycle of Toxoplasma gondii. In Weiss LM & Kim K (eds.), Toxoplasma gondii, the Model Apicomplexan: Perspectives and Methods. London, UK. Academic Press. 2007, p 1-17.
  2. Montoya JG, Liesenfeld O. Toxoplasmosis. Lancet 2004; 363: 1965-1976. https://doi.org/10.1016/S0140-6736(04)16412-X
  3. Tracey KJ. The inflammatory reflex. Nature 2002; 420: 853-859. https://doi.org/10.1038/nature01321
  4. Miller CM, Boulter NR, Ikin RJ, Smith NC. The immunobiology of the innate response to Toxoplasma gondii. Int J Parasitol 2009; 39: 23-39. https://doi.org/10.1016/j.ijpara.2008.08.002
  5. Da Silva AS, Monteiro SG, Gonçalves JF, Spanevello R, Schmatz R, Oliveira CB, Costa MM, França RT, Jaques JA, Schetinger MR, Mazzanti CM, Lopes ST. Trypanosoma evansi: Immune response and acetylcholinesterase activity in lymphocytes from infected rats. Exp Parasitol 2011; 127: 475-480. https://doi.org/10.1016/j.exppara.2010.10.017
  6. Da Silva CB, Wolkmer P, Da Silva AS, Paim FC, Tonin AA, Castro VS, Felin DV, Schmatz R, Gonçalves JF, Badke MR, Morsch VM, Mazzanti CM, Lopes ST. Cholinesterases as markers of the inflammatory process in rats infected with Leptospira interrogans serovar. icterohaemorrhagiae. J Med Microbiol 2012; 61: 278-284. https://doi.org/10.1099/jmm.0.035501-0
  7. Das UN. Acetylcholinesterase and butyrylcholinesterase as possible markers of low-grade systemic inflammation. Med Sci Monit 2007; 13: 214-221.
  8. Borovikova LV, Ivanova S, Zhang M, Yang H, Botchkina GI, Watkins LR, Wang H, Abumrad N, Eaton JW, Tracey KJ. Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin. Nature 2000; 405: 458-462. https://doi.org/10.1038/35013070
  9. Kawashima K, Fujii T. The lymphocytic cholinergic system and its contribution to the regulation of immune activity. Life Sci 2003; 74: 675-696. https://doi.org/10.1016/j.lfs.2003.09.037
  10. Kimura R, Ushiyama N, Fujii T, Kawashima K. Nicotine-induced Ca2+ signaling and down-regulation of nicotinic acetylcholine receptor subunit expression in the CEM human leukemic T-cell line. Life Sci 2003; 72: 2155-2158. https://doi.org/10.1016/S0024-3205(03)00077-8
  11. Li B, Stribley JA, Ticu A, Xie W, Schopfer LM, Hammond P, Brimijoin S, Hinrichs SH, Lockridge O. Abundant tissue butyrylcholinesterase and its possible function in the acetylcholinesterase knockout mouse. J Neurochem 2000; 75: 1320-1331.
  12. Schetinger MRC, Porto NM, Moretto MB, Morsch VM, Da Rocha JBT, Vieira V, Moro F, Neis RT, Bittencourt S, Bonacorso HG, Zanatta N. New benzodiazepines alter acetylcholinesterase and ATPDase activities. Neurochem Res 2000; 25: 949-955. https://doi.org/10.1023/A:1007500424392
  13. Feldman BF, Zinkl JG & Jain NC. Schalm's Veterinary Hematology. 5th ed. Philadelphia, USA. Lippincott Williams & Wilkins. 2000.
  14. Boyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest (suppl.) 1968; 97: 77-89.
  15. Strober W. Trypan blue exclusion test of cell viability. In Current Protocols in Immunology, A.3B.1-A.3B.2. Edited by J. Coligan, A. Kruisbeek, D. Marguiles, E. Shevach & W. Strober, 2001.
  16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 1976; 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  17. Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  18. Worek F, Mast U, Kiderlen D, Diepold C, Eyer P. Improved determination of acetylcholinesterase activity in human whole blood. Clin Chim Acta 1999; 288: 73-90. https://doi.org/10.1016/S0009-8981(99)00144-8
  19. Fitzgerald BB, Costa LG. Modulation of muscarinic receptors and acetylcholinesterase activity in lymphocytes and in brain areas following repeated organophosphate exposure in rats. Fundam Appl Toxicol 1993; 20: 210-216. https://doi.org/10.1006/faat.1993.1028
  20. Evans TG, Schwartzman JD. Pulmonary toxoplasmosis. Semin Respir Infect 1991; 6: 51-57.
  21. Tayebati SK, El-Assouad D, Ricci A, Amenta F. Immunochemical and immunocytochemical characterization of cholinergic markers in human peripheral blood lymphocytes. J Neuroimmunol 2002; 132: 147-155. https://doi.org/10.1016/S0165-5728(02)00325-9
  22. Szelenyi J, Paldi-Haris P, Hollan S. Changes in the cholinergic system of lymphocytes due to mitogenic stimulation. Immunol Lett 1987; 16: 49-54. https://doi.org/10.1016/0165-2478(87)90060-5
  23. Czura CJ, Tracey KJ. Autonomic neural regulation of immunity. J Intern Med 2005; 257: 156-166. https://doi.org/10.1111/j.1365-2796.2004.01442.x
  24. Nizri E, Hamra-Amitay Y, Sicsic C, Lavon I, Brenner T. Anti-inflammatory properties of cholinergic up-regulation: a new role for acetylcholinesterase inhibitors. Neuropharmacology 2006; 50: 540-547. https://doi.org/10.1016/j.neuropharm.2005.10.013
  25. Da Silva AS, Tonin AA, Thorstenberg ML, Leal DB, Fighera R, Flores MM, França RT, Camillo G, Vogel FS, de la Rue M, Lopes ST. Relationship between butyrylcholinesterase activity and liver injury in mice acute infected with Toxoplasma gondii. Pathol Res Pract 2013; 209: 95-98. https://doi.org/10.1016/j.prp.2012.10.007

피인용 문헌

  1. Is There a Cholinergic Survival Incentive for Neurotropic Parasites in the Brain? vol.8, pp.12, 2013, https://doi.org/10.1021/acschemneuro.7b00370