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Effects of Seed Pre-treatment and Seedling Culture System on Germination and Subsequent Growth of Cynanchum wilfordii

종자 전처리 및 육묘방법이 큰조롱의 종자 발아 및 생육에 미치는 영향

  • 이수광 (국립수목원 산림자원보존과) ;
  • 조원우 (동국대학교 바이오환경과학과) ;
  • 구자정 (국립수목원 산림자원보존과) ;
  • 강호덕 (동국대학교 바이오환경과학과)
  • Received : 2012.10.09
  • Accepted : 2013.02.04
  • Published : 2013.02.28

Abstract

The present study was examined seed germination and growth characteristics of Cynanchum wilfordii under the following conditions such as soaking temperature, concentrations of $GA_3$, trays and soil types, and shading conditions. In seed pre-treatment, germination rate was the highest at 93% when soaked in 100 ppm $GA_3$ at $20^{\circ}C$ and seedling growth was best in 50 plug cell tray. Physiological quality was best in the condition in which seeds were treated with 10 ppm $GA_3$ at $20^{\circ}C$. Taking into consideration the seedling growth, their physiological quality as well as economical aspects, seedlings with plant height (over 10 cm) and root length (over 10 cm) were grown vigorously 30-45 days after the seeds were sown in mid-April in TKS, TKS+perlite and TKS+rice hull of 128 or 200 plug cell tray.

본 연구는 점차 사라져가는 큰조롱의 유전자원을 보호하고 이용하고자 종자 발아 기술과 육묘기술 개발을 위해 종자 침지 처리 온도, $GA_3$ 농도, 트레이 및 토양 종류, 차광처리에 따른 큰조롱의 발아율과 생육특성을 조사하였다. 종자발아는 $20^{\circ}C$에서 $GA_3$ 100 ppm에 침지한 처리구에서 가장 높은 93% 발아율을 보였다. 묘소질 특성은 50구 트레이에서 가장 뛰어났으며 생리적 묘소질 특성은 $20^{\circ}C$에서 $GA_3$ 10 ppm에 침지 처리 한 종자를 TKS 토양이 충진된 128구 트레이에 파종한 조건에서 가장 뛰어났다. 따라서 큰조롱의 묘소질과 생리적 묘소질을 종합하고 경제적인 측면을 고려하면 128구 트레이 혹은 200구 트레이의 TKS, TKS+펄라이트 및 TKS+왕겨 처리구에서 4월 중순에 파종 후 30~ 45일 간 육묘하면 초장과 근장이 각각 10 cm 이상되고 엽수가 8 매 이상 되는 우량한 묘를 획득할 수 있었고 이를 본 밭에 이식이 가능한 것으로 보여진다.

Keywords

References

  1. Chang, K.C. and D.U. Lee. 2000. Vasodilatory effect of the alkaloid component from the roots of Cynanchum wilfordii Hemsley. Korean J. Life Sci. 10:584-590 (in Korean).
  2. Chang, Y.D., J.H. Jeong and C.H. Lee. 2009. Effect of plug cell size and seedling periods on seedling quality of Dendranthema zawadskii var. latilobum (Maxim.) Kitam. Korean J. Plant Academic Conference 2009:128 (in Korean).
  3. Choi, I.S., S.Y. Son, J.S. Park, J.T. Cho, I.M. Chung and J.H. Lee. 1995. Effect of planting density on growth and yield in Cynanchum wilfordii. Korean J. Plant. Res. 8:209-215 (in Korean).
  4. Choi, I.S., S.Y. Son, J.T. Cho, J.S. Park, D.H. Han and I.M. Chung. 1996. Effect of seeding date on the growth and yield of Cynanchum wilfordii Hemsley. Korean J. Medical Crop Sci. 4:114-118 (in Korean).
  5. Choi, J.K. 2001. Our medicinal grass, flower, tree 1. Hann moonhwa-multimedia. Publishing Co., Seoul, Korea. pp. 242-253 (in Korean).
  6. Gibson, J.L. 2001. Plant propagation: Basic principles and methodology. NC State University Floriculture Research 8:1-6.
  7. Ham, I.H., J.Y. Lee, Y.J. Yoon, G.S. Yang, Y.M. Bu, H.C. Kim and H.Y. Choi. 2007. Effects of Cynanchum spp. on the hyperlipidemia in rats induced by Triton WR-1339. Korean. J. Herbology 22:279-286 (in Korean).
  8. Han, J.S., S.K. Kim, S.W. Kim and Y.J. Kim. 2001. Effects of shading treatments and harvesting methods on the growth of Eleutherococcus senticosus Maxim. Korean J. Medical Crop Sci. 9:1-7 (in Korean).
  9. Han, K.S., G.J. Shin, W.C. Lee and J.H. Lee. 1998. Study on the antioxidative effects and amino acid contents of the roots of Cynanchum wilforii. J. Korean Soc. Ori. Internal Medicine 19:411-430 (in Korean).
  10. Hartmann, H.T., D.E. Kester, F.T. Davies and R.L. Geneve. 1997. Plant propagation principles and practices. 6th ed. Prentice Hall, Englewood Cliffs, USA. p. 770.
  11. Hwang, I.S., J.H. Yoo, E.S. Seong, J.G. Lee, H.Y. Kim, N.J. Kim, J.D. Lee, J.K. Ham, Y.S. Ahn, N.Y. Kim and C.Y. Yu. 2012. The effect of temperature and seed soaking on germination in Cynanchum wilfordii (Maxim.) Hemsl. Korean J. Medical Crop Sci. 20:136-139 (in Korean). https://doi.org/10.7783/KJMCS.2012.20.2.136
  12. Hwang, I.T., K.C. Cho, B.S. Kim, H.K. Kim, J.G. Kim and K.S. Kim. 2007. Effects of root substrate, preplanting nutrient charge and tray cell size on the quality and growth of Chrysanthemum (Dendranthema grandiflorum L.) cuttings. Korean Flower Res. J. 45:131-135 (in Korean).
  13. Kang, J.H., K.J. Jeong, K.O. Choi, Y.S. Chon and J.G. Yun. 2010. Morphological characteristics and germination as affected by low temperature and GA in orostachys 'Jirisan' and 'Jejuyeonhwa' seeds, Korea native plant. Korean J. Hor. Sci. Technol. 28:913-920 (in Korean).
  14. Kang, J.H., S.Y. Yoon and S.H. Jeon. 2004. Review: Analysis on practicality of seed treatments for medicinal plants published in Korean scientific journals. Korean J. Medical Crop Sci. 12:328-341 (in Korean).
  15. Kang, J.H., Y.J. Kim and B.S. Jeon. 2001. Effects of presown cold stratification, $GA_{3}\;KNO_{3}$, and acetone treatment on germination of Crotalaria sessiflora L. Korean J. Medical Crop Sci. 9:124-129 (in Korean).
  16. Kellogg, C.H., S.D. Bridgham and S.A Leicht. 2003. Effects of water level, shade and time on germination and growth of freshwater marsh plants along a simulated successional gradient. J. Ecology 91:274-282. https://doi.org/10.1046/j.1365-2745.2003.00764.x
  17. Kim, M.J., B.G. Park, S.H. Park and S.G. Park. 1999. Labor-saving cultivation of Cynanchum wilfordii using support crops. Korean J. Plant. Res. 12:204-208 (in Korean).
  18. Kim, M.J., I.J. Kim, S.Y. Nam, C.H. Lee and B.H. Song. 2002. Effects of sowing method and planting density on growth and root yield of Cynanchum wilfordii Hemsly. Korean J. Crop Sci. 47:418-421 (in Korean).
  19. Kim, S.H., J.H. Saung, Y.K. Kim, and P.G. Kim. 2008. Photosynthetic responses of four oak species to changes in light environment. Korean J. Agric. For. Meteorol. 10:141-148 (in Korean). https://doi.org/10.5532/KJAFM.2008.10.4.141
  20. Kim, Y.H. and H.S. Park. 2002. Growth of cucumber plug seedlings as affected by photoperiod and photosynthetic photon flux. Korean J. Bio-Envir. Control. 11:40-44 (in Korean).
  21. Koran Meteorological Administration Homepage. 2012. www.kma.go.kr
  22. Lee, C.B. 1980. Korea illustrated plant book. Hyangmoonsa, Seoul, Korea. p. 630 (in Korean).
  23. Lee, D.U. 1995. Antioxidative effects of the crude alkaloid fraction from Cynanchum wilfordii. Journal of Dongguk. 14:83-92 (in Korean).
  24. Lee, D.U. and W.C. Lee. 1997. An 5-Lipoxygenase inhibitor iolated from the roots of Cynanchum wilfordii Hemsley. Korean J. Pharmacogn. 28:247-251 (in Korean).
  25. Lee, J.S., W.G. Pill. S.H. Park, Y.A. Shin, G.R. Do and Y.K. Kang. 2003. Effect of seed treatment and seedling culture system on germination and subsequent growth 'King of Denmark' spinach (Spinacia olercea). Korean J. Hor. Sci. Technol. 44:850-854 (in Korean).
  26. Lee, S.C., J.S. Lee and S.J. Jeong. 2005. Germination and seedling growth of Miscanthus sacchariflorus as influenced by different plug cells and medium composition. Korean J. Hor. Sci. Technol. 23:315-318 (in Korean).
  27. Lee, S.G., S.H. Lee and H.D. Kang. 2011. In vitro propagation of wild Cynanchum wilfordii through axillary bud culture. Jour. Korean For. Soc. 100:172-177 (in Korean).
  28. Lee, Y.J. and Y.J. Son. 1999. The effects of Polygoni multiflori radix and Cynanachi wilfordii radix on the blood lipids and enzymes of hypercholesterolic rats. Korean J. Herbology. 14:69-77 (in Korean).
  29. Park, H.J., D.H. Chung, S.G. Kim and B.S. Kwon. 1995. Influences of sowing time and nursery period on growth and yield of Perilla frutescens BRITTON var. acuta KUDO. Korean J. Medical Crop Sci. 3:1-4 (in Korean).
  30. Park, K.W., H.W. Baeck. M.S. Kim and M.J. Lee. 1998. Selection of media on the germination and growth of herbs. Korean J. Hor. Sci. Technol. 16:492 (in Korean).
  31. Parkhill, J.P., G. Maillet and J.J. Cullen. 2001. Fluorescence based maximal quantum yield for PSII as a diagnostic of nutrient stress. J. Phycol. 37:517-529. https://doi.org/10.1046/j.1529-8817.2001.037004517.x
  32. Qu, Y.H., W.M. Wei, Y.F. Hou, B. Chen, G.Q. Chen and C. Lin. 2009. Analysis for an environmental friendly seedling breeding system. Commun Nonlinear Sci. Numer Simulat. 14:1766-1772. https://doi.org/10.1016/j.cnsns.2008.03.020
  33. Seong, M.K., H.S. Kwon, G.U. Ji and J.U. Lee. 2004. Cynanchum wilfordii intake of fermented products on the effect of chemically induced colon cancer. Conference of Korean Nutrition. p. 177 (in Korean).
  34. Shin, M.K. 1985. A comparative study on the effects of polygoni radix and cynanchi radix on rat livers intoxicated with carbon tetrachloride. Korean J. Pharmacogn. 16:81-92 (in Korean).
  35. U, Z.K., S.J. Song and U. Hansen. 1994. Stress effects on photosynthesis of greenhouse plants as measured by the fluorescence method. Korean J. Environ. Agric. 13:183-190.
  36. Won, J.Y., C.Y. Lee, D.J. Oh and S.M. Kim. 2008. Changes of chlorophyll fluorescence and photosynthesis under different shade materials in Korean ginseng (Panax ginseng C. A. Meyer). Korean J. Medical Crop Sci. 16:416-420 (in Korean).

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