Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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PAMAM Dendrimers Conjugated with L-Arginine and γ-Aminobutyric Acid as Novel Polymeric Gene Delivery Carriers

  • Son, Sang Jae (Graduate School of Analytical Science and Technology (GRAST), Chungnam National University) ;
  • Yu, Gwang Sig (Department of Biochemistry, College of Natural Sciences, Chungnam National University) ;
  • Choe, Yun Hui (Department of Biochemistry, College of Natural Sciences, Chungnam National University) ;
  • Kim, Youn-Joong (Graduate School of Analytical Science and Technology (GRAST), Chungnam National University) ;
  • Lee, Eunji (Graduate School of Analytical Science and Technology (GRAST), Chungnam National University) ;
  • Park, Jong-Sang (School of Chemistry and Molecular Engineering, Seoul National University) ;
  • Choi, Joon Sig (Graduate School of Analytical Science and Technology (GRAST), Chungnam National University)
  • Received : 2012.10.17
  • Accepted : 2012.11.29
  • Published : 2013.02.20
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Abstract

In this study, we synthesized functional dendrimer derivatives as nonviral gene delivery vectors. Poly(amidoamine) dendrimer (PAMAM, generation 4) was modified to possess functional amino acids to enhance gene transfection efficiency. PAMAM G4 derivatives conjugated with L-arginine (Arg) and ${\gamma}$-aminobutyric acid (GABA) showed higher transfection efficiency and lower cytotoxicity compared to the native PAMAM G4 dendrimer. The polyplex of the PAMAM G4 derivative/pDNA was evaluated using an agarose gel retardation assay and Picogreen reagent assay. Additionally, the MTT assay was performed to examine the cytotoxicity of synthesized polymers. All PAMAM G4 derivatives showed lower cytotoxicity than PEI25kD. Particularly, PAMAM G4-GABA-Arg displayed enhanced transfection efficiency compared to the native PAMAM G4 dendrimer.

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References

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