Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Comparison of fibrinolytic activity from Korean indigenous insects

국내 토착 곤충의 항혈전 비교

  • Kim, Hyunae (National Academy of Agricultural Science, RDA) ;
  • Lee, Sang Han (School of Food Science & Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Choi, Youngcheol (National Academy of Agricultural Science, RDA) ;
  • Park, Kwanho (National Academy of Agricultural Science, RDA) ;
  • Hwang, Jaesam (National Academy of Agricultural Science, RDA) ;
  • Kim, Namjung (National Academy of Agricultural Science, RDA) ;
  • Nam, Sunghee (National Academy of Agricultural Science, RDA)
  • 김현애 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) ;
  • 이상한 (경북대학교 농업생명과학대학 식품공학부) ;
  • 최영철 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) ;
  • 박관호 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) ;
  • 황재삼 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) ;
  • 김남정 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) ;
  • 남성희 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과)
  • Received : 2013.09.08
  • Accepted : 2013.10.23
  • Published : 2013.11.30
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Abstract

The fibrinolytic activity of aqueous extracts from Korean indigenous insects were studied. Fibrinolytic activity of aqueous extract from 5 insects (larva and adult parts of Meloimorpha japonica, Allomyrina dichotoma, Cetonia pilifera, Apis mellifera) showed 2.7-fold potent than that of plasmin used as a positive control. In addition, the fibrinolytic activity was observed through the degradation products by SDS-PAGE. The extracts efficiently hydrolyzed ${\alpha}-$, ${\beta}-$ and ${\gamma}$ chains of human fibrinogen. This study suggested that aqueous extracts from Korean indigenous insects have potential in developing a useful source of antithrombosis agent(s).

국내 유용 곤충의 70% 에탄올 및 물 추출물의 혈전용해 효과를 확인하기 위하여 fibrin plate를 이용하여 혈전용해능을 측정하였고, 그 분해산물을 SDS-PAGE를 이용하여 분리하였다. 그 결과 5종 (방울벌레 유충, 방울벌레 성충, 장수풍뎅이 성충, 꽃무지 유충, 꿀벌 유충)의 물 추출물에서 plasmin과 비교하여, 높은 혈전 용해 능을 나타내었고, 분해 패턴 또한 plasmin과 비슷하거나 더 강한 단백질 분해능을 나타내어 물 추출물에서 높은 혈전 용해능을 확인할 수 있었다.

Keywords

References

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