한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제26권1호
  • /
  • Pages.50-62
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  • 2013
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
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자초(紫草)가 HaCaT 세포의 사이토카인 유전자 발현에 미치는 영향

Effects of Lithospermum erythrorhizon on the cytokine gene expression in human keratinocytes

  • 강상훈 (가천대학교 한의과대학 안이비인후피부과 교실) ;
  • 김경준 (가천대학교 한의과대학 안이비인후피부과 교실)
  • Kang, Sang-Hoon (Department of Ophthalmology, Otolaryngology and Dermatology, College of Oriental Medicine, Gachon University) ;
  • Kim, Gyung-Jun (Department of Ophthalmology, Otolaryngology and Dermatology, College of Oriental Medicine, Gachon University)
  • 투고 : 2013.01.18
  • 심사 : 2013.02.14
  • 발행 : 2013.02.25
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초록

Objective : Propionibacterium acnes (P. acnes) is a major pathogenic bacteria for acne vulgaris. This study was performed to evaluate the effects of Lithospermum erythrorhizon extracts on the inflammatory cytokines gene expression by P. acnes in human keratinocytes, HaCaT cell line. Methods : Anti-bacterial activity and cytotoxicity of LE extracts was analyzed by agar plate culture and XTT assay. The cytokines gene expressions were assessed by real time RT-PCR for IL-8, MCP-1 and TNF-${\alpha}$. During the cell culture and treatments, amounts of secreted TNF-${\alpha}$ were measured by ELISA. Translocation of transcription factor NF-${\kappa}B$ from cytoplasm into nucleus was observed by immunocytochemistry and confocal microscopy. Results : There were no anti-bacterial effects and cytotoxicity as high as $1,000{\mu}g/ml$ of LE extracts in XTT assay. Transcription levels of inflammatory cytokines, IL-8, MCP-1 and TNF-${\alpha}$ were increased by P. acnes in HaCaT. LE extracts decreased the upregulated gene transcription levels. However, amounts of secreted TNF-${\alpha}$ were similar in HaCaT cells with P. acnes and LE extracts. Translocation of NF-${\kappa}B$ into nucleus by P. acnes was significantly inhibited by LE extracts. Conclusions : From the results of this study, LE extracts have anti-inflammatory effects on HaCaT cells by P. acnes that decreased the mRNA expressions of IL-8, MCP-1 and TNF-${\alpha}$. This anti-inflammatory effects of LE extracts could provide the potential of therapeutic substance for acne vulgaris.

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