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  • 제13권1호
  • /
  • Pages.10-15
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  • 2013
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  • 1598-2629(pISSN)
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  • 2092-6685(eISSN)
대한면역학회 (The Korean Association of Immunobiologists)
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Alum Directly Modulates Murine B Lymphocytes to Produce IgG1 Isotype

  • Jin, Bo-Ra (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Kim, Sun-Jin (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Lee, Jeong-Min (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Kang, Seong-Ho (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Han, Hye-Ju (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Jang, Young-Saeng (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Seo, Goo-Young (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University) ;
  • Kim, Pyeung-Hyeun (Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University)
  • 투고 : 2013.01.07
  • 심사 : 2013.01.25
  • 발행 : 2013.02.28
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초록

Aluminum hydroxide (alum) is the most widely used adjuvant in human vaccines. Nevertheless, it is virtually unknown whether alum acts on B cells. In the present study, we explored the direct effect of alum on Ig expression by murine B cells in vitro. LPS-activated mouse spleen B cells were cultured with alum, and the level of isotype-specific Ig secretion, IgG1 secreting cell numbers, and Ig germ-line transcripts (GLT) were measured using ELISA, ELISPOT, and RT-PCR, respectively. Alum consistently enhanced total IgG1 production, numbers of IgG1 secreting cells, and $GLT{\gamma}1$ expression. These results demonstrate that alum can directly cause IgG1 isotype switching leading to IgG1 production.

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참고문헌

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