환경생물 (Korean Journal of Environmental Biology)

  • 제31권4호
  • /
  • Pages.376-382
  • /
  • 2013
  • /
  • 1226-9999(pISSN)
  • /
  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지
DOI QR코드

DOI QR Code

[6]-Gingerol Attenuates Radiation-induced Cytotoxicity and Oxidative Stress in HepG2 Cells

  • Chung, Dong-Min (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Uddin, S.M. Nasir (University of Science & Technology) ;
  • Kim, Jin Kyu (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 투고 : 2013.10.23
  • 심사 : 2013.11.20
  • 발행 : 2013.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

[6]-Gingerol, a major polyphenol of ginger (Zingiber officinale), exhibits a variety of biological properties including anti-oxidant, anti-inflammatory and anti-cancer activity. However, the radioprotective effect of [6]-gingerol is still unknown. The aim of this study was to investigate the radioprotective effect of [6]-gingerol against radiation-induced cell cytotoxicity and oxidative stress in HepG2 cells. [6]-Gingerol pretreatment attenuated radiation-induced cell cytotoxicity caused by 5Gy (half lethal dose, $LD_{50}$ of HepG2 cells). The measurements of superoxide dismutase (SOD) and catalase (CAT) activity were also performed. The results showed that [6]-gingerol pretreatment reduced increasing SOD and CAT activity after exposure of IR, indicating that [6]-gingerol protected oxidative stress by regulating cellular antioxidant enzyme (SOD and CAT) activity. These findings suggest that [6]-gingerol acts as a radioprotector by attenuating cell cytotoxicity and oxidative stress.

키워드

참고문헌

  1. Alia M, S Ramos, R Mateos, AB Granado-Serrano, L Bravo and L Goya. 2006. Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide. Toxicol. Appl. Pharmacol. 212:110-118. https://doi.org/10.1016/j.taap.2005.07.014
  2. Bak MJ, M Jun and WS Jeong. 2012. Antioxidant and Hepatoprotective effects of the red ginseng essential oil in $H_2O_2$-treated HepG2 cells and $CCl_4$-treated mice. Int. J. Mol. Sci. 12:2314-2330.
  3. Bradford MM. 1976. Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  4. Chakraborty D, A Mukherjee, S Sikdar, A Paul, S Ghosh and AR Khuda-Bukhsh. 2012. [6]-Gingerol isolated from ginger attenuates sodium arsenite induced oxidative stress and plays a corrective role in improving insulin signaling in mice. Toxicol. Lett. 210:34-43. https://doi.org/10.1016/j.toxlet.2012.01.002
  5. Chmielewski AG and MH Saeid. 2004. Radiation technologies: past, present and future. Radiat. Phys. Chem. 71:16-20.
  6. Curtin JF, M Donovan and TG Cotter. 2002. Regulation and measurement of oxidative stress in apoptosis. J. Immunol. Methods 265:49-72. https://doi.org/10.1016/S0022-1759(02)00070-4
  7. Devasagayam TPA, JC Tilak, KK Boloor, KS Sane, SS Ghaskadbi and RD Lele. 2004. Free radical and Antioxidants in human health: Current status and future prospects. JAPI 52:794-804.
  8. Dugasani S, MR Pichika, VD Nadarajah, MK Balijepalli, S Tandra and JN Korlakunta. 2010. Comparative antioxidant and anti-inflammatory effects of [6]-Gingerol, [8]-Gingerol, [10]-Gingerol and [6]-Shogaol. J. Ethnopharmacol. 127:515-520. https://doi.org/10.1016/j.jep.2009.10.004
  9. Duthie SJ, W Johnson and VL Dobson. 1997. The effect of dietary flavonoids on DNA damage (strand breaks and oxidized pyrimidines) and growth in human cells. Mutat. Res. 390:141-151. https://doi.org/10.1016/S0165-1218(97)00010-4
  10. Held KD. 1997. Radiation induced apoptosis and its relationship to loss of clonogenic survival. Apoptosis 2:265-282. https://doi.org/10.1023/A:1026485003280
  11. Hosseinimehr SJ. 2007. Foundation review: Trends in the development of radioprotective agents. Drug Discovery Today. 12:794-805. https://doi.org/10.1016/j.drudis.2007.07.017
  12. Jauregui HO, NT Hayner, JL Driscoll, W Holland, MH Lipsky and PM Galletti. 1981. Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes freshly isolated cells, cell suspensions, and primary monolayer cultures. In vitro 17:1100-1110. https://doi.org/10.1007/BF02618612
  13. Kim EC, JK Min, TY Kim, SJ Lee, HO Yang, S Han, YM Kim and YG Kwon. 2005. [6]-Gingerol, a pungent ingredient of ginger, inhibits angiogenesis in vitro and in vivo. Biochem. Biophys. Res. Commun. 335:300-308. https://doi.org/10.1016/j.bbrc.2005.07.076
  14. Kim JK, Y Kim, KM Na, YJ Surh and TY Kim. 2007. 6-Gingerol prevents UVB-induced ROS production and COX-2 expression in vitro and in vivo. Free Radic. Res. 41:603-614. https://doi.org/10.1080/10715760701209896
  15. Kunwar A and KI Priyadarsini. 2011. Free radicals, oxidative stress and importance of antioxidants in human health. J. Med. Allied Sci. 1:53-60.
  16. Lee C, GH Park, CY Kim and JH Jang. 2011. [6]-Gingerol attenuates ${\beta}$-amyloid induced oxidative cell death via fortifying cellular antioxidant defense system. Food Chem. Toxicol. 49:1261-1269. https://doi.org/10.1016/j.fct.2011.03.005
  17. Lipkin M, B Reddy, H Newmark and SA Lamprecht. 1999. Dietary factors in human colorectal cancer. Annu. Rev. Nutr. 19:545-586. https://doi.org/10.1146/annurev.nutr.19.1.545
  18. Lv L, H Chen, D Soroka, X Chen, TC Leung and S Sang. 2012. 6-Gingerdiols as the major metabolites of 6-Gingerol in cancer cells and in mice and their cytotoxic effects on human cancer cells. J. Agric. Food Chem. 60:11372-11377. https://doi.org/10.1021/jf303879b
  19. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  20. Moulder JE. 2004. Post irradiation approaches to treatment of radiation injuries in the context of radiological terrorism and radiation accidents: a review. Int. J. Radiat. Biol. 80:3-10. https://doi.org/10.1080/09553000310001642920
  21. Nigam N, K Bhui, S Prasad, J George and Y Shukla. 2009. [6]-Gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells. Chem. Biol. Interact. 181:77-84. https://doi.org/10.1016/j.cbi.2009.05.012
  22. Noroozi M, WJ Angerson and MEJ Lean. 1998. Effects of flavonoids and vitamin-C on oxidative DNA damage to human lymphocytes. Am. J. Clin. Nutr. 67:1210-1218.
  23. Riley PA. 1994. Free radicals in biology: oxidative stress and effects of ionizing radiation. Int. J. Radiat. Biol. 65:27-33. https://doi.org/10.1080/09553009414550041
  24. Ryan JL. 2012. Ionizing radiation: The good, the bad and the ugly. J. Invest. Dermatol. 132:985-993. https://doi.org/10.1038/jid.2011.411
  25. Shukla Y and M Singh. 2007. Cancer preventive properties of ginger: A brief review. Food Chem. Toxicol. 45:683-690. https://doi.org/10.1016/j.fct.2006.11.002
  26. Shukla Y, S Prasad, C Tripathi, M Singh, J George and N Kalra. 2007. In vitro and in vivo modulation of testosterone mediated alterations in apoptosis related proteins by [6]-gingerol. Mol. Nutr. Food Res. 51:1492-1502. https://doi.org/10.1002/mnfr.200700197
  27. Weiss JF and MR Landauer. 2003. Protection against ionizing radiation by antioxidant nutrients and phytochemicals. Toxicology 189:1-20. https://doi.org/10.1016/S0300-483X(03)00149-5
  28. White B. 2007. Ginger: An overview. American Family Physician 75:1690-1691.