Journal of the Korean Society of Manufacturing Process Engineers (한국기계가공학회지)

The Korean Society of Manufacturing Process Engineers (한국기계가공학회)
권호 표지

Characteristic of neuroblastoma cell (SH-SY5Y) culture on the crystalline diamond film

다결정 다이아몬드 필름의 신경종양세포(SH-SY5Y) 배양 특성

  • Nam, Hyo-Geun (Dept. of Medical IT Engineering, Kumoh National Institute of technology) ;
  • Oh, Hong-Gi (Dept. of Medical IT Engineering, Kumoh National Institute of technology) ;
  • Kim, Dae-Hoon (Dept. of Medical IT Engineering, Kumoh National Institute of technology) ;
  • Kim, Min-Hye (Dept. of Medical IT Engineering, Kumoh National Institute of technology) ;
  • Park, Hye-Bin (Dept. of Applied chemistry, Kumoh National Institute of technology) ;
  • Jhee, Kwang-Hwan (Dept. of Applied chemistry, Kumoh National Institute of technology) ;
  • Song, Kwang-Soup (Dept. of Medical IT Engineering, Kumoh National Institute of technology)
  • Published : 2013.08.30
⟨ Previous Next ⟩

Abstract

In order to fabricate high sensitive and stable biosensors, we require the material with superior biocompatibility and physical-chemical stability. Many kinds of biomaterials have been evaluated to apply for bioindustry. Recently, carbon based diamond thin films have been focal pointed as bio-applications and their possibility has been evaluated. Diamond thin film has many advantages for electrochemical and biological applications, such as wide potential window (3.0-3.5V), low background current and chemical-physical stability. In this work, we have cultured neuroblastoma cell (SH-SY5Y) on the crystalline diamond films. We use MTT assay to evaluate the characteristic of cell culture on the substrates. As a result, neuroblastoma cell was cultured on the crystalline diamond film as similar as cell culture dish.

Keywords

References

  1. K. S. Song, Y. Nakamura, Y. Sasaki, M. Degawa, J. H. Yang and H. Kawarada, "pH-sensitive diamond field-effect transistors (FETs) with directly aminated channel surface", Analytica Chimica Acta, 5, 573-574, 2006.
  2. B. Rezek, L.Michalikova, E. Ukraintsev, A. Kromka and M. Kalbacova, "Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces", sensors, 9, 3549-3562, 2009. https://doi.org/10.3390/s90503549
  3. M.Amara, P.S.Gomes, M.A.Lopes, J.D.Santos , R.F.Silva and M.H.Fernandes, Cytotoxicity evaluation of nanocrystalline diamond coatings by fibroblast cell cultures", 5, 755-763, 2009.
  4. G.P.Moreno and D.J.Riley, "The influence of surface preparation on the electrochemistry of boron doped diamond: A study of the reduction of 1,4-benzoquinone in acetonitrile", Electrochemistry Communications, 4, 218-221, 2002. https://doi.org/10.1016/S1388-2481(02)00255-2
  5. K.W.Chae, Y.J. Baik, J.K.Park and W.S.Lee, "The 8-inch free-standing CVD diamond wafer fabricated by DC-PACVD", Diamond and Related Materials, 19, 1168-1171, 2010. https://doi.org/10.1016/j.diamond.2010.05.002
  6. Q.Su, W.Shi, D.Li, J.Lai, L.Jiang, L.Wang, W.Ding and Y.Xia, "Effect of carbon concentration on properties of nano-diamond films", Applied surface Science, 258, 4645-4648, 2012. https://doi.org/10.1016/j.apsusc.2012.01.047
  7. B.Rezek, M.Kratka, A.Kromka and M.Kalbacova, "Effect of protein inter-0layers on cell-diamond FET characteristics", Biosensors and Bioelectronics, 26, 1307-1312, 2010. https://doi.org/10.1016/j.bios.2010.07.027
  8. F.Liu, J.Wang, B.Liu, X.Li and D.Chen, "Effect of surface termination on electronic structure of nano-crystalline diamond film", Proceedings of the 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems, China, January 18 - 21, 2006.
  9. M.Kalbacova, B.rezek, V.Baresova, C. Wolf- Brandstetter and A.Kromka, "Nanoscale topography of nanocrystalline diamonds promotes differentiation of osteoblasts", Acta Biomaterialia, 5, 3076-3085, 2009. https://doi.org/10.1016/j.actbio.2009.04.020
  10. Y.C.Chen, D.C.Lee, C.Y.Hsiao, Y.F.Chung, H.C. Chen, J.P.Thomas, W.F.Pong, N.H.Tai, I.N.Lin and I.M.Chiu, "The effett of ultra-nanocrystalline diamond films on the proliferation and differentiation of neural stem cells", Biomaterials, 30, 3428-3435, 2009. https://doi.org/10.1016/j.biomaterials.2009.03.058