Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Phosphate Removal Using Recombinant Bacteria with Cytoplasmic Phosphate Binding Protein

Phosphate Binding Protein이 세포질에 발현된 재조합 박테리아를 이용한 인 제거

  • Choi, Suk Soon (Department of Biological and Environmental Engineering, Semyung University) ;
  • Ha, Jeong Hyub (School of Environmental Science and Technology, Pohang University of Science and Technology) ;
  • Cha, Hyung Joon (Department of Chemical Engineering, Pohang University of Science and Technology)
  • 최석순 (세명대학교 바이오환경공학과) ;
  • 하정협 (포항공과대학교 환경공학부) ;
  • 차형준 (포항공과대학교 화학공학과)
  • Published : 2013.10.31
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Abstract

In the present work, we constructed a recombinant Escherichia coli with cytoplasmic-expressed phosphate-binding protein (PBP) and investigated its phosphate removal in water phase. When the recombinant bacteria were cultured for 6 h to treat phosphate, the removal efficiencies were 90, 49, and 41% for the treatment of 1.0, 1.5, and 2.0 mg/L phosphate, respectively, indicating good specific phosphate removal of our developed system. Also, cell densities of 2.5 and 5.0 Optical density resulted in high phosphate removal efficiencies and ~80% of 2.0 mg/L phosphate was efficiently removed. A novel biotechnology developed in this study could be effectively employed for resolving eutrophication problem in water body.

본 연구에서는 인 결합 단백질(phosphate-binding protein)이 세포질(cytoplasm)에서 발현되는 재조합 대장균(Escherichia coli)을 제조하였으며, 이를 이용하여 수중에 함유된 인의 제거에 대하여 고찰하였다. 이 균주를 6 h 동안 배양하여 1.0, 1.5, 2.0 mg/L의 인을 처리하였을 때, 각각 90, 49, 41%의 제거효율을 도출하였으며, 우수한 인 제거 능력(specific phosphate removal)을 나타내었다. 또한, 세포의 Optical density를 2.5, 5.0으로 조절하여 운전하였을 때, 인의 제거효율이 향상됨을 알 수 있었으며, 2.0 mg/L 인을 80%까지 제거시킬 수 있었다. 이 연구를 통하여 얻어진 새로운 생물기술은 수계의 부영양화 문제를 해결하는데 효과적으로 사용될 수 있을 것이다.

Keywords

References

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