Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지

Chungyangeum Attenuated the Allergic Inflammation in vivo and in vitro

  • Kim, Su-Jin (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Lee, Jae-Ho (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Oh, Chung Hwan (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Oh, Sa-Rang (College of Pharmacy, Keimyung University) ;
  • Jung, Ji-Wook (Department of Herbal Medicinal Pharmacology, Daegu Haany University)
  • Received : 2013.08.28
  • Accepted : 2013.12.25
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Chungyangeum (CYE) is a newly designed herbal drug formula for the purpose of treating atopic dermatitis. The aim of the present study is to elucidate whether and how CYE modulates the allergy inflammation in vitro and in vivo. We investigate to ascertain the pharmacological effects of CYE on both compound 48/80 or histamine-induced scratching behaviors and 2, 4-dinitrochlrobenzene (DNCB)-induced atopic dermatitis in mice. Additionally, we attempted to determine the effects of CYE on lipopolysaccharide (LPS)-induced inflammatory responses in macrophages. The findings of this study demonstrated that CYE reduced compound 48/80 or histamine-induced scratching behaviors and DNCB-induced atopic dermatitis in mice. The CYE inhibited the production of inflammatory cytokines as well as the activation of NF-${\kappa}B$ and caspase-1 in stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of CYE as a potential molecule for use in the treatment of allergic inflammation diseases.

Keywords

References

  1. Allam JP, Novak N. The pathophysiology of atopic eczema. Clin Exp Dermatol. 2006. 31: 89-93. https://doi.org/10.1111/j.1365-2230.2005.01980.x
  2. Bauernfeind FG, Horvath G, Stutz A, Alnemri ES, MacDonald K, Speert D, Fernandes-Alnemri T, Wu J, Monks BG, Fitzgerald KA, Hornung V, Latz E. Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression. J Immunol. 2009. 183: 787-791. https://doi.org/10.4049/jimmunol.0901363
  3. Boero S, Silvestri M, Ullmann N, Rossi GA. Modulation by flunisolide of tumor necrosis factor-alpha-induced stimulation of airway epithelial cell activities related to eosinophil inflammation. J Asthma. 2010. 47: 381-387. https://doi.org/10.3109/02770901003759410
  4. Brenninkmeijer EE, Spuls PI, Legierse CM, Lindeboom R, Smitt JH, Bos JD. Clinical differences between atopic and atopiform dermatitis. J Am Acad Dermatol. 2008. 58: 407-414. https://doi.org/10.1016/j.jaad.2007.12.002
  5. Buske-Kirschbaum A, Geiben A, Hellhammer D. Psychobiological aspects of atopic dermatitis: an overview. Psychother Psychosom. 2001. 70: 6-16. https://doi.org/10.1159/000056219
  6. Druilhe A, Srinivasula SM, Razmara M, Ahmad M, Alnemri ES. Regulation of IL-1beta generation by Pseudo-ICE and ICEBERG, two dominant negative caspase recruitment domain proteins. Cell Death Differ. 2001. 8: 649-657. https://doi.org/10.1038/sj.cdd.4400881
  7. Faubel S, Lewis EC, Reznikov L, Ljubanovic D, Hoke TS, Somerset H, Oh DJ, Lu L, Klein CL, Dinarello CA, Edelstein CL. Cisplatin-induced acute renal failure is associated with an increase in the cytokines interleukin (IL)-1beta, IL-18, IL-6, and neutrophil infiltration in the kidney. J Pharmacol Exp Ther. 2007. 322: 8-15. https://doi.org/10.1124/jpet.107.119792
  8. Gadaleta RM, Oldenburg B, Willemsen EC, Spit M, Murzilli S, Salvatore L, Klomp LW, Siersema PD, van Erpecum KJ, van Mil SW. Activation of bile salt nuclear receptor FXR is repressed by pro-inflammatory cytokines activating NF-_{\kappa}B signaling in the intestine. Biochim Biophys Acta. 2011. 1812: 851-858. https://doi.org/10.1016/j.bbadis.2011.04.005
  9. Gilmore TD, Garbati MR. Inhibition of NF-${\kappa}B$ signaling as a strategy in disease therapy. Curr Top Microbiol Immunol. 2011. 349: 245-263.
  10. Gold MS, Kemp AS. Atopic disease in childhood. Med J Aust. 2005. 182: 298-304.
  11. Humke EW, Shriver SK, Starovasnik MA, Fairbrother WJ, Dixit VM. ICEBERG: a novel inhibitor of interleukin-1beta generation. Cell. 2000. 103: 99-111. https://doi.org/10.1016/S0092-8674(00)00108-2
  12. Kim SJ, Chung WS, Kim SS, Ko SG, Um JY. Antiinflammatory effect of Oldenlandia diffusa and its constituent, hentriacontane, through suppression of caspase-1 activation in mouse peritoneal macrophages. Phytother Res. 2011. 25: 1537-1546. https://doi.org/10.1002/ptr.3443
  13. Lee SH, Stehlik C, Reed JC. Cop, a caspase recruitment domaincontaining protein and inhibitor of caspase-1 activation processing. J Biol Chem. 2001. 276: 34495-34500. https://doi.org/10.1074/jbc.M101415200
  14. Leung DY, Bieber T. Atopic dermatitis. Lancet. 2003. 361: 151-160. https://doi.org/10.1016/S0140-6736(03)12193-9
  15. McCormick TS, Stevens SR, Kang K. Macrophages and cutaneous inflammation. Nat Biotechnol. 2000. 18: 25-26. https://doi.org/10.1038/71879
  16. Minami K, Kamei C. A chronic model for evaluating the itching associated with allergic conjunctivitis in rats. Int Immunopharmacol. 2004. 4: 101-108. https://doi.org/10.1016/j.intimp.2003.10.013
  17. Mukaida N. Interleukin-8: an expanding universe beyond neutrophil chemotaxis and activation. Int J Hematol. 2000. 72: 391-398.
  18. Shiohara T, Hayakawa J, Mizukawa Y. Animal models for atopic dermatitis: are they relevant to human disease? J Dermatol Sci. 2004. 36: 1-9. https://doi.org/10.1016/j.jdermsci.2004.02.013
  19. Taxman DJ, Holley-Guthrie EA, Huang MT, Moore CB, Bergstralh DT, Allen IC, Lei Y, Gris D, Ting JP. The NLR adaptor ASC/ PYCARD regulates DUSP10, mitogen-activated protein kinase (MAPK), and chemokine induction independent of the inflammasome. J Biol Chem. 2011. 286: 19605-19616. https://doi.org/10.1074/jbc.M111.221077
  20. Tegeder I, Pfeilschifter J, Geisslinger G. Cyclooxygenaseindependent actions of cyclooxygenase inhibitors. FASEB J. 2001. 15: 2057-2072. https://doi.org/10.1096/fj.01-0390rev
  21. Trefzer U, Hofmann M, Sterry W, Asadullah K. Cytokine and anticytokine therapy in dermatology. Expert Opin Biol Ther. 2003. 3: 733-743. https://doi.org/10.1517/14712598.3.5.733
  22. Wang X, Wang H, Figueroa BE, Zhang WH, Huo C, Guan Y, Zhang Y, Bruey JM, Reed JC, Friedlander RM. Dysregulation of receptor interacting protein-2 and caspase recruitmentdomain only protein mediates aberrant caspase-1 activation in Huntington's disease. J Neurosci. 2005. 25: 11645-11654. https://doi.org/10.1523/JNEUROSCI.4181-05.2005