한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제23권3호
  • /
  • Pages.27-35
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  • 2013
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  • 1229-1854(pISSN)
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  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
권호 표지

무기환(戊己丸)의 RAW 264.7 세포에 대한 항염증작용 연구

Anti-inflammatory Effect of Mugi-hwan Water Extract in RAW 264.7 Cells

  • 김일현 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 최종환 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이세원 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학과교실)
  • Kim, Ilhyun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Chonghwan (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Lee, Sewon (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Song, Yungsun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
  • 발행 : 2013.07.31
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives The aim of this study was to investigate anti-inflammatory activity of Mugi-hwan (MGH) Water Extract. Methods Cells were treated with 2 ug/ml of LPS 1 hour prior to the addition of MGH. Cell viability was measured by MTS assay. The production of NO was determined by reacting cultured medium with Griess reagent. The expression of COX-2, iNOS and MAPKs was investigated by Western blot, RT-PCR. The content of level of cytokines ($PGE_2$, IL-6, in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. Results MGH inhibited the production of NO, $PGE_2$, IL-6 as well as the expressions of iNOS, COX-2 in the murine macrophage, RAW 264.7 cells. MGH also had suppression effects of LPS induced MAPKs activation. Conclusions These results suggest that MGH has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.

키워드

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