Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Identification of GABA-producing Microorganism from Chungkookjang

GABA 함량이 높은 청국장을 발효하는 균주의 분리 및 동정

  • Mann, So-Yon (Department of Food Science & Technology, Pusan National University) ;
  • Kim, Eun-Ah (Department of Food Science & Technology, Pusan National University) ;
  • Lee, Ga-Young (Department of Food Science & Technology, Pusan National University) ;
  • Kim, Ro-Ui (Department of Food Science & Technology, Pusan National University) ;
  • Hwang, Dae-Youn (Department of Biomaterial Science, Pusan National University) ;
  • Son, Hong-Joo (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Kim, Dong-Seob (Department of Food Science & Technology, Pusan National University)
  • Received : 2012.10.30
  • Accepted : 2013.01.03
  • Published : 2013.01.30
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Abstract

To isolate GABA-producing microorganisms, 1,500 strains were isolated from different Chungkookjang samples and screened. From these strains, 20 were selected for further analyses based on a protease and slime-producing activity test. The MC 31 strain showed the highest GABA concentration in Chungkookjang and was used in this study. MC 31 was identified as Bacillus subtilis by an API 50CHB kit and 16S rDNA sequences analysis and named as B. subtilis MC 31. B. subtilis MC 31 showed exponential growth up to 12 hours at $37^{\circ}C$ in LB broth, and it reached a stationary phase after 24 to 36 hours of incubation. B. subtilis MC 31 showed maximum GABA content at 72 hours after incubation at $40^{\circ}C$.

전통적인 방법으로 제조된 청국장으로부터 우수한 발효 균주들을 분리하여 재래식으로 청국장을 제조하고, 제조된 청국장의 생리활성 물질인 GABA를 유지 및 보강하면서 품질을 향상시키기 위하여 청국장 발효능이 뛰어난 종균을 찾아 분리하였다. 분리된 균주들 가운데 GABA의 함량이 높은 청국장을 생산하는 MC 31을 실험균주로 선택하였고 API Kit와 16S rDNA sequence를 통하여 Bacillus subtilis MC 31로 명명하였다. B. subtilis MC 31의 최적배지와 온도, 시간을 찾아본 결과 LB 배지에서 $37^{\circ}C$, 24시간이 가장 높은 생육을 나타내었다. GABA 생산에 적합한 발효 온도와 시간을 조절하여 최적 조건을 찾아본 결과 B. subtilis MC 31는 $40^{\circ}C$에서 72시간에 가장 많은 GABA를 생산하였다.

Keywords

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