농업과학연구 (Korean Journal of Agricultural Science)

  • 제40권2호
  • /
  • Pages.139-146
  • /
  • 2013
  • /
  • 2466-2402(pISSN)
  • /
  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
권호 표지
DOI QR코드

DOI QR Code

황색포도상구균 신속 검출용 간이 진단키트

Rapid Detection Kit for Staphylococcus aureus

  • 김기영 (국립농업과학원 농업공학부) ;
  • 박샛별 (국립농업과학원 농업공학부) ;
  • 문지혜 (국립농업과학원 농업공학부) ;
  • 이상대 (국립농업과학원 농업공학부) ;
  • 이새롬 (국립농업과학원 농업공학부) ;
  • 장윤정 (국립농업과학원 농업공학부)
  • Kim, Gi-Young (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Park, Saet-Byeol (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Moon, Ji-Hea (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Lee, Sang-Dae (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Lee, Sea-Rom (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Jang, Youn-Jung (Department of Agricultural Engineering, National Academy of Agricultural Sciences)
  • 투고 : 2013.04.01
  • 심사 : 2013.05.15
  • 발행 : 2013.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was performed to develop a rapid test kit for pathogenic Staphylococcus in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and Staphylococcus antibodies were used as a tag and a receptor, respectively. Manually spotted Staphylococcus antibody and anti-mouse antibody on the surface of nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Staphylococcus aureus in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Staphylococcus spiked in PBS. Also, the rapid test kit could detect $10^5$ cfu/mL of Staphylococcus in chicken meat extract.

키워드

참고문헌

  1. MFDS (Ministry of Food and Drug Safety). 2010. Assessed in http://www.foodnara.go.kr/foodnara/downManager?a=series &b=1309221903295&c=1&d=sub06 on 29 March 2013a.
  2. MFDS (Ministry of Food and Drug Safety). 2012. Assessed in http://www.kfda.go.kr/e-stat/index.do?nMenuCode=33 on 29 March 2013b.
  3. Cui S, Tong G. 2008. A chromatographic strip test for rapid detection of one lineage of the H5 subtype of highly pathogenic avian influenza. Journal of Veterinary Diagnostic Investigation. Journal of Veterinary Diagnostic Investigation 20:567-571. https://doi.org/10.1177/104063870802000505
  4. Hossain ZSM, Luckham RE, McFadden MJ, Brennan JD. 2009. Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. Analytical Chemistry 81:9055-9064. https://doi.org/10.1021/ac901714h
  5. Kang BK, Oh JS, Lee CS, Park BK, Park YN, Hong KS, Lee KG, cho BK, Song DS. 2007. Evaluation of a rapid immunodiagnostic test kit for rabies virus. Journal of Virological Methods 145:30-36. https://doi.org/10.1016/j.jviromet.2007.05.005
  6. Kim GY, Yang GM, Park SB, Kim YH, Lee KJ, Son JY, Kim HJ, Lee SR. 2011. Rapid Detection Kit for Salmonella typhimurium. Journal of Biosystems Engineering 36:140-146. https://doi.org/10.5307/JBE.2011.36.2.140
  7. Martin-Hernandez C, Munoz M, Daury C, Weymuth H, Kemmers-Voncken AEM, Corbaton V, Toribio T, Bremer MGEG. 2009. Immunochromatographic lateral-flow test strip for the rapid detection of added bovine rennet whey in milk and milk power. International Dairy Journal 19: 205-208. https://doi.org/10.1016/j.idairyj.2008.10.016
  8. Moon JH, Kim GY, Lee SD. 2012. A Gold Nanoparticle and Aflatoxin B1-BSA Conjugates Based Lateral Flow Assay Method for the Analysis of Aflatoxin B1. Materials 5:634-643. https://doi.org/10.3390/ma5040634
  9. Oh JS, Ha GW, Cho YS, Kim MJ, An DJ, Hwang KK, Lim YK, Park BK, Kang BK, Song DS. 2006. One-Step Immunochromatography assay kit for detecting antibodies to canine parvovirus. Clinical and Vaccine Immunology 13:520-524. https://doi.org/10.1128/CVI.13.4.520-524.2006
  10. Park SB, Kim GY, Moon JH. 2012. Improvement of Lateral Flow Assay for the rapid detection of Salmonella. Food Engineering Progress 16:333-339.
  11. Sithigorngul W, Rukpratanporn S, Sittidilokratna N, Pecharaburanin N, Longyant S, Chaivisuthangkura P, Sithigorngul P. 2007. A convenient immunochromatographic test strip for rapid diagnosis of yellow head virus infection in shrimp. Journal of Virological Methods 140:193-199. https://doi.org/10.1016/j.jviromet.2006.11.034
  12. Tang Y, Zhai YF, Xiang JJ, Wang H, Liu B, Guo CW. 2010. Colloidal gold probe-based immunochromatographic assay for the rapid detection of lead ions in water samples. Environmental Pollution 158:2074-2077. https://doi.org/10.1016/j.envpol.2010.03.001