Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Shoot regeneration via culture of leaf explants in pear (Pyrus pyrifolia cv. Niitaka)

잎 절편 배양을 이용한 배 '신고' (Pyrus pyrifolia cv. Niitaka)의 신초 재분화

  • Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Dae Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Ki Ok (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Hyang Bun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Do, Kyung Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chun, Jae An (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hwang, Hae Seong (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 김세희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 신일섭 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 조강희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김대현 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김현란 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김기옥 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 이향분 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 도경란 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 천재안 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 황해성 (농촌진흥청 국립원예특작과학원 과수과)
  • Received : 2013.11.13
  • Accepted : 2013.11.27
  • Published : 2013.12.31
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Abstract

Genetic manipulation of pear (Pyrus pyrifolia Nakai) breeding is still difficult due to lack of reliable regeneration system. The aim of this research is to establish shoot regeneration system from leaf explants for pear (P. pyrifolia cv. Niitaka) using various concentrations of plant growth regulators and carbon source supplemented to medium. The highest regeneration rate of about 20% was found on a medium containing 4.4 g/L of Murashige and Skoog (MS) without vitamins, Linsmaier and Skoog (LS) vitamins were added separately. Leaf explants of pear were cultured on MS medium containing 7 g/L of Daishin agar supplemented with various concentrations of NAA (0.01, 0.05, 0.1, 0.5 mg/L) in combination with BA(3, 5, 10 mg/L) for shoot regeneration. In medium with 5 mg/L of BA and 0.01 mg/L of NAA, adventitious shoot regeneration rate was higher than others treated. The optimal results were observed using MS medium supplemented with 30 g/L sorbitol as carbon source on regeneration system. Sorbitol is considered better carbon source than sucrose for shoot regeneration of pear (P. pyrifolia cv. Niitaka). In order to increase of shoot regeneration in pear (P. pyrifolia cv. Niitaka), plant agar and Daishin agar used as gelling agents, Daishin agar is more efficient in shoot regeneration.

남방형 동양배(Pyrus pyrifolia Nakai)의 형질전환은 적합한 재분화 체계가 구축되어 있지 않아 어려운 실정이다. 본 연구의 목적은 배 신고 품종(P. pyrifolia cv. Niitaka)의 효율적인 재분화 체계를 개발하기 위해 식물생장조절제의 농도, 탄소원, 지지체 등이 신초 재분화에 미치는 영향을 연구하기 위하여 수행하였다. 생장조절제의 영향을 알아보기 위하여 NAA 0.01, 0.05, 0.1, 0.5 mg/L과 BA 3, 5, 10 mg/L을 조합하여 처리한 결과 BA 5 mg/L와 NAA 0.01 mg/L의 조합에 Daishin agar 7 g/L과 Linsmaier and Skoog (LS) vitamin을 따로 첨가한 Murashige and Skoog (MS) 배지에서 재분화율이 20%로 나왔다. 효율적인 재분화 체계를 위한 탄소원으로는 sorbitol 30 g/L가 사용되었으며 배 '신고'의 재분화에는 sucrose보다 sorbitol이 더 적합한 것으로 판단된다. 재분화가 잘 되지 않는 배 '신고'의 신초 재분화율을 높이기 위해 지지체로 plant agar와 Daishin agar를 사용하였으며 Daishin agar에서 재분화율이 더 높게 나타났다.

Keywords

References

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