Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Studies of interesterification properties of irradiated lipases

방사선 조사선량에 따른 lipase의 interesterification 반응특성 변화 연구

  • Lee, Chi-Woo (Department of Food Science and Technology, Chungnam National University) ;
  • Heo, Yoon-Ji (Department of Food Science and Technology, Chungnam National University) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
  • Received : 2013.08.08
  • Accepted : 2013.09.16
  • Published : 2013.09.30
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Abstract

This study was conducted to prove the effect of irradiation on lipases (lipase AK, lipase AH, lipase PS-D, Lipozyme TLIM, Lipozyme RMIM and Novozyme SP435) which were used for interesterification reaction using batch type reactor. Through such interesterification, structured lipid (1(3)-palmitoyl-2-oleoyl-3(1)-stearoyl, POS) was synthesized by lipase treated with irradiation at different doses (0, 3, 7, 14, 29 and 59 kGy) using canola oil, palmitic ethyl ester (PEE) and stearic ethyl ester (StEE). After the reaction, fatty acid composition of triacylglycerol (TAG) in structured lipid was analyzed to compare the lipase activity. The results showed that activity of the irradiated lipase AH, PS-D and Novozyme SP435 with certain dose (3 kGy) were slightly improved. Such change of lipase activity suggested that irradiation might affect on the interesterification properties. Especially, Lipase AK, Lipozyme TLIM and Lipozyme RMIM after at 3 kGy irradiation showed that content of stearic acid ($C_{18:0}$) was increased while palmitic acid ($C_{16:0}$) decreased in the interesterified products.

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References

  1. Association of Official Analytical Chemists(AOAC). 2001. Official Method 996.06 Fat(total, saturated, and unsaturated) in foods.
  2. Cho SW, Rhee JS. 1993. Immobilization of lipase for effective interesterification of fats and oils in organic solvent. Biotechnology and Bioengineering 41(2):204-210. https://doi.org/10.1002/bit.260410206
  3. Farkas J. 1998. Irradiation as a method for decontaminating food A review. International Journal of Food Microbiology 44(3):189-204. https://doi.org/10.1016/S0168-1605(98)00132-9
  4. Jaeger KE, Ransacb S, Dijkstrab BW, Colson C, Van Heuvel M, Misset O. 1994. Bacterial lipases. FEMS Microbiology Reviews 151:29-63.
  5. Lee KT, Akoh CC. 1996. Immobilized lipase-catalyzed production of structured lipids with eicosapentaenoic acid at specific positions. Journal of the American Oil Chemists' Society 73(5):611-615. https://doi.org/10.1007/BF02518116
  6. Lee KT, Akoh CC. (1998). Structured lipids: synthesis and applications. Food Reviews International 14(1):17-34. https://doi.org/10.1080/87559129809541148
  7. Lim SI, Yook HS, Yoon HH, Kim YJ, Byun MW. 1998. Effect of gamma irradiation on egg white protein. Journal of the Korean Society of Food Science and Nutrition 27(2):291-295.
  8. Macrae AR. 1983. Lipase-catalyzed interesterification of oils and fats. Journal of the American Oil Chemists' Society 60(2):291-294. https://doi.org/10.1007/BF02543502
  9. Moon JH, Lee JH, Shin JA, Hong ST, Lee KT. 2011. Optimization of lipase-catalyzed production of structured lipids from canola oil containing similar composition of triacylglycerols to cocoa butter. Journal of The Korean Society of Food Science and Nutrition 40(10):1430-1437. https://doi.org/10.3746/jkfn.2011.40.10.1430
  10. Posorske LH 1984. Industrial-scale application of enzymes to the fats and 12. oil industry. Journal of the American Oil Chemists' Society 61(11):1758-1760. https://doi.org/10.1007/BF02582143
  11. SAS Institute, Inc. 2002. SAS/STAT User's Guide release 9.01. Statistical analysis systems institute. Cary NC, USA.
  12. Stamatis H, Xenakis A, Provelegiou M, Kolisis FN, 1993. Esterification reactions catalyzed by lipases in micro-emulsions: The role of enzyme localization in relation to its selectivity. Biotechnology and Bioengineering 42:103-110. https://doi.org/10.1002/bit.260420114
  13. Sung NY, Choi JI, Song BS, Kim JK, Lee JW, Byun MW, Kim MJ, Kim HJ. 2011. Tumor cell cytotoxicity of gamma irradiated chaga mushroom extract. Journal of Radiation Industry 5(1):35-39.
  14. Valivety, Rao H., Peter J. Halling, Alan D. Peilow, and Alasdair R. Macrae. 1992. Lipases from different sources vary widely in dependence of catalytic activity on water activity. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology 1122(2):143-146. https://doi.org/10.1016/0167-4838(92)90316-6
  15. Wetzel K, Huebner G, Baer M. 1985. Irradiation of onions, spices and enzyme solutions in the German Democratic Republic. In INTERNATIONAL ATOMIC ENERGY AGENCY. Food irradiation processing: proceedings of an international symposium. Washington. pp. 4-8.
  16. Wisdom RA, Dunnill P, Lilly MD, Macrae A. 1984. Enzymic interesterification of fats: factors influencing the choice of support for immobilized lipase. Enzyme and Microbial Technology 6(10):443-446. https://doi.org/10.1016/0141-0229(84)90093-0
  17. Yook HS, Lee HJ, Im SI, Kim S, Byun MW. 1997. Changges of proteolytic enzyme property by gamma irradiation. Journal of The Korean Society of Food Science and Nutrition 26(6):1116-1121.