농업과학연구 (Korean Journal of Agricultural Science)

  • 제40권3호
  • /
  • Pages.221-226
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  • 2013
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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Serratia marcescens S3-R1이 생산한 효소에 의한 유청단백질 가수분해물의 특성과 면역조절 활성

Whey protein hydrolytic properties and its immunomodulation activity by produced enzyme from Serratia marcescens S3-R1

  • 유재민 (충남대학교 농업생명과학대학 동물바이오시스템과학과) ;
  • 렌친핸드 (충남대학교 농업생명과학대학 동물바이오시스템과학과) ;
  • 정석근 (농촌진흥청 국립축산과학원) ;
  • 배형철 (충남대학교 농업생명과학대학 동물바이오시스템과학과) ;
  • 남명수 (충남대학교 농업생명과학대학 동물바이오시스템과학과)
  • Yu, Jae Min (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Renchinkhand, G. (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jeong, Seok Geun (National Institute of Animal Science, Rural Development Administration) ;
  • Bae, Hyoung Churl (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Nam, Myoung Soo (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University)
  • 투고 : 2013.09.12
  • 심사 : 2013.09.16
  • 발행 : 2013.09.30
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초록

Degrees of hydrolysis by alkaline protease produced from Serratia marcescens S3-R1 is 3.95-6.30% of whey proteins during 5, 15, 30, 60, 90, 120,180, 240 min incubation at $40^{\circ}C$. Proteolytic pattern of the whey proteins showed that various low molecular weight peptides were generated during the incubation periods. The biological function of in Raw 264.7 cells treated with whey protein hydrolytic peptides, anti-inflammatory effect showed exhibit in the expression of pro-inflammatory cytokines such as TNF-${\alpha}$, IL-6, COX-2 and iNOS by PCR analysis. COX-2 and iNOS gene expression inhibited in Raw 264.7 cells on whey protein hydrolysates below 3,000 dalton. The protease from Serratia marcescens S3-R1 showed a potential in production of low molecular weight whey protein hydrolysates which could be used for industrial application.

키워드

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