농약과학회지 (The Korean Journal of Pesticide Science)

  • 제17권3호
  • /
  • Pages.178-184
  • /
  • 2013
  • /
  • 1226-6183(pISSN)
  • /
  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지
DOI QR코드

DOI QR Code

침투이행성 농약의 토양처리 후 토마토잎에서의 잔류에 의한 꿀벌 독성

Honeybee Toxicity by Residues on Tomato Foliage of Systemic Insecticides Applied to the Soil

  • Bae, Chul-Han (Agricultural Reasearch Center, Hankooksamgong Co. Ltd.) ;
  • Cho, Kyung-Won (Agricultural Reasearch Center, Hankooksamgong Co. Ltd.) ;
  • Kim, Yeon-Sik (Agricultural Reasearch Center, Hankooksamgong Co. Ltd.) ;
  • Park, Hyun-Ju (Agricultural Reasearch Center, Hankooksamgong Co. Ltd.) ;
  • Shin, Kwan-Seop (Agricultural Reasearch Center, Hankooksamgong Co. Ltd.) ;
  • Park, Yeon-Ki (Rural Development Adimistration) ;
  • Lee, Kyu-Seung (Dept. of Bio Envrionmental Chemistry, Chungnam National University)
  • 투고 : 2013.04.22
  • 심사 : 2013.08.30
  • 발행 : 2013.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

꿀벌에 대한 급성독성이 강한 침투이행성 농약을 대상으로 토양처리 후 흡수이행에 의해 나타나는 꿀벌(Apis mellifera)에 대한 영향을 조사하고자 꿀벌 엽상잔류독성시험을 수행하였다. 본 연구에서는 침투이행성 농약인 dinotefuran 입제와 imidacloprid 입제 그리고 clothianidin 입제를 토마토 정식시 3가지 처리량으로 토양에 파구처리를 하였다. 처리 후 경과시간에 따라 잎을 채취하여 잎에서의 잔류농도를 분석하였고 꿀벌에 노출시켜 꿀벌치사율을 조사하였다. Dinotefuran 입제 처리구의 꿀벌 독성은 7일 이후 꿀벌치사율이 100%로 나타났고 17일 이후 감소하였다. Imidacloprid 입제의 경우 꿀벌 최대치사율이 44~72%로 낮았으나 그 영향은 18일까지 지속된 후 감소하였고 clothianidin 입제는 처리 후 7일에 꿀벌치사율이 100%이었으며 14일 이후 점차 감소하였다. 시험농약 처리 후 경과시간에 따른 잎의 잔류 경향성과 각 시험농약에서 꿀벌의 치사율은 유사한 경향성을 보였으며, 토마토의 생장이 활발한 14일 이후에는 토마토 잎에서의 잔류량이 점차 감소하였고, 실제 사용량 처리시 21일 이후에는 엽상잔류에 의한 꿀벌의 영향도 확연하게 감소하였다.

Residual toxicity test to honeybee was conducted to evaluate an indirect effects on honeybee after planting hole application of systemic insecticides which were highly toxic to honeybee (Apis mellifera). In this study, It was applied three application rates in the planting hole by three systemic insecticides, dinotefuran GR, imidacloprid GR and clothianidin GR at planting time of tomato. Residual analysis of foliage was carried out after periodic sampling the foliage and investigated the effects of exposed honeybee on the tomato foliage. The honeybee mortality by dinotefuran residues on the foliage was shown almost 100% at 7 days after treatment and decreased 17 days after treatment. The maximum mortality of honeybee by imidacloprid residues on the foliage was 44 ~ 72%. But the effect of pesticide lasted for 18 days and then decreased. The honeybee mortality by clothianidin residues on the foliage was 100% at 7 days after treatment and decreased 14 days after treatment. A tendency of the honeybee mortality and residue in foliage showed a similar character as time goes by. The residues in tomato foliage decreased gradually after 14 days by vigorous growth of tomatoes and the toxic effect of honeybee was significantly decreased after 21 days in actual usage of the treatment.

키워드

참고문헌

  1. Andrea, T., D. Marton, C. Giorio, A. Zanella, L. Solda, M. Marzaro, L. Vivan and V. Girolami (2012) Assessment of the environmental exposure of honeybees to particulate matter containing neonicotinoid insecticides coming from corn coated seeds. Environmental Science & Technology 46(5):2592-2599. https://doi.org/10.1021/es2035152
  2. Benjamin, A., (2008) Pesticides:Germany bans chemicals linked to honeybee devastation, by The Guardian/UK.
  3. Bortolotti, L., R. Monanari, J. Marcelino and P. Porrini (2003) Effects of sub-lethal imidacloprid doses on the homing rate and foraging activity of honey bees. Bulletin of Insectology 56(1):63-7.
  4. Bruneau E., J. Kievits, J. Lortsch and S. Szoke (2009) Systemic plant protection substances and products: how to assess the risk for bees? A beekeepers point of view, II. Test and risk assessment (incl. systemic effects, field testing, bee brood), Hazards of pesticides to bees - 10th International Symposium of the ICP-Bee Protection Group, 55-66.
  5. Defra (2009) Systemic pesticide risk assessement for honeybees - research project final report, Science directorate, management support and finance team.
  6. Dennis V. E., C. -F. Diana, F. Maryann, O. Nancy and H. Jerry (2006) Colony Collapse Disorder Preliminary Report. Mid-Atlantic Apiculture Research and Extension Consortium (MAAREC) CCD Working Group. p. 22.
  7. EFSA Scientific Report (2006) Conclusion regarding the peer review of the pesticide risk assessment of the active substance fipronil. 65:1-110.
  8. EPA (2011) EPA Home > Pesticides > About Pesticides > Pesticide issues in the works Pesticide issues in the works: Honeybee colony collapse disorder, Current as of December 16, 2011.
  9. Featherstone, D. (2009) Microbiology: Colony collapse cured?. Nature 458(7241):949.
  10. Halm M. P., A. Rortais, G. Arnold, J. N. Tasei and S. Rault (2006) New risk assessment approach for systemic insecticides: The case of honey bees and imidacloprid (Gaucho), Environ. Sci. Technol., 40(7):2448-2454. https://doi.org/10.1021/es051392i
  11. Johnson, R. M., J. D. Evans, G. E. Robinson, and M. R. Berenbaum (2009) Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera). Proceedings of the National Academy of Sciences 106(35): 14790 https://doi.org/10.1073/pnas.0906970106
  12. KCPA (2009) Using guideline of crop protection agents.
  13. Oldroyd, B. P. (2010) What's killing American honey bees? PLoS Biol 5(6):e168.
  14. Pettis S., D. V. Engelsdorp, J. Johnson and G. Dively (2012) Pesticide exposure in honey bees results in increased levels of the gut pathogen Nosema. Naturwissenschaften 99(2): 153-158. https://doi.org/10.1007/s00114-011-0881-1
  15. Schmuck R., R. Schoning, A. Stork and O. Schramel (2001) Risk posed to Honeybees (Apis mellifera L, Hymenoptera) by an Imidacloprid seed dressing of sunflowers-Pest Manag Sci 57:225-238. https://doi.org/10.1002/ps.270
  16. Standards of Pesticide Registration, Standards and Methods for Ecotoxicity Testing, Honeybee toxicity of residues on foliage (Rural Development Administration Notification No. 2012-13).
  17. Szalanski, A. L., J. Whitaker and P. Cappy (2010) Molecular diagnostics of Nosema ceranae and N. apis from honey bees in New York. Proceedings of the American Bee Research Conference. American Bee Journal 150:508.
  18. Tasei, J. N., M. Ha, P. Delegue and L. Belzunces (2003) Registration of systemic insecticides and european and mediterranean plant protection organisation (EPPO) guidelines, Bulletin of Insetology 56(1):189-191.
  19. The Pesticide Manual (2006).
  20. USEPA (1996) Ecological effect test guidelines OPPTS 850.3030 Honey bee toxicity of residue on foliage. Washington DC, USA.

피인용 문헌

  1. Residual Characteristics of a Systemic Insecticide Flonicamid and Its Metabolites in Sweet Pepper vol.18, pp.4, 2014, https://doi.org/10.7585/kjps.2014.18.4.228
  2. Simultaneous determination of flonicamid, imidacloprid, and its metabolites in paprika by QuEChERS and tandem mass spectrometry vol.58, pp.4, 2013, https://doi.org/10.1007/s13765-015-0083-1
  3. Inspection of the Fragmentation Pathway for Thiamethoxam vol.8, pp.3, 2013, https://doi.org/10.5478/msl.2017.8.3.65
  4. Foliage Residual Toxicity to Honeybee on Pepper and Cucumber Foliage of Systemic Insecticides by Root Uptake Into Crops vol.23, pp.1, 2013, https://doi.org/10.7585/kjps.2018.23.1.8
  5. Survey on the Pesticide Residues in the Soil After Harvesting Broccoli, Head Lettuce and Lettuce vol.24, pp.4, 2020, https://doi.org/10.7585/kjps.2020.24.4.361