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Changes in Fermentative Characteristics of Red Ginseng Wine using Enzymatically Hydrolyzed Red Ginseng Marc

홍삼박 효소분해물을 이용하여 제조한 홍삼주의 발효특성 변화

  • Kim, Dong Chung (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University)
  • Received : 2013.08.30
  • Accepted : 2013.12.05
  • Published : 2013.12.31

Abstract

This study examined the fermentative characteristics of red ginseng wine prepared with enzymatically hydrolyzed red ginseng marc (ERGM), which was produced by the polysaccharide hydrolyase and protease treatments of red ginseng marc (RGM). After adjusting the initial soluble solid contents of ERGM broth to $20^{\circ}Brix$ and $30^{\circ}Brix$ with sucrose, respectively, alcohol fermentation was carried out at $25^{\circ}C$ for 9 days. The soluble solid contents decreased from $20^{\circ}Brix$ to $7^{\circ}Brix$ and from $30^{\circ}Brix$ to $13^{\circ}Brix$, and the reducing sugar content decreased to 18 and 24 g/L at 9 days of fermentation, respectively. The ethanol contents increased to 65 g/L and 106 g/L, respectively. The crude saponin content in the ERGM broth was 2~3 times as high as that in the RGM broth after 7 days fermentation. These results show that ERGM is suitable as a raw material for the production of red ginseng wine.

복합 탄수화물 분해효소와 단백질 분해효소로 처리하여 제조한 홍삼박 효소분해물에 효모를 발효시킨 홍삼주의 발효특성을 조사하였다. 초기 가용성 고형분 함량을 $20^{\circ}Bx$$30^{\circ}Bx$로 조정한 후 효모를 접종하고 $25^{\circ}C$에서 발효시험을 실시한 결과, 발효 9일 후 가용성 고형분 함량은 $7.5^{\circ}Bx$$13.1^{\circ}Bx$로, 환원당 함량은 18 g/L와 24 g/L까지 감소하였다. 에탄올 함량은 당 소비량과 비례하여 최종 에탄올 함량은 각각 65 g/L와 106 g/L까지 증가하였다. pH는 모든 실험구에서 발효 2일째까지 급격하게 감소하여 pH 4.73에서 pH 3.99로 낮아졌으며, 그 이후 9일째까지 pH의 변화는 미미하였다. 적정산도는 pH 변화와 비례하여 증가하였다. 7일간 발효시킨 후 홍삼주의 조사포닌 함량은 효소 처리하지 않은 대조구에 비하여 100~200% 향상되었다. 그러므로 홍삼박 효소분해물은 홍삼 발효주의 원료로 이용 가능함을 확인하였다.

Keywords

References

  1. A. S. Attele, J. A .Wu, C. S. Yuan, "Ginseng pharmacology: multiple constituents and multiple actions". Biochem. Pharmacol. Vol. 58, pp. 1685-1693, 1999. DOI: http://dx.doi.org/10.1016/S0006-2952(99)00212-9
  2. Y. S. Kwak, J. D. Park, J. W. Yang, "Present and its prospect of red ginseng efficacy research". Food Ind. Nutr. Vol. 8, pp. 30-37, 2003.
  3. H. S. Sung, S. K. Yoon, W. J. Kim, C. B. Yang, "Relationship between chemical components and their yields of red ginseng extract by various extracting conditions". Korean J. Ginseng Res. Vol. 9, pp. 170-178, 1985.
  4. S. D. Lee, G. Yoo, H. J. Chae, M. J. In, N. S. Oh, Y. K. Hwang, W. I. Hwang, D. C. Kim DC, "Lipid-soluble extracts as the main source of anticancer activity in ginseng and ginseng marc". J. Am. Oil Chem. Soc. Vol. 86, pp. 1065-1071, 2009. DOI: http://dx.doi.org/10.1007/s11746-009-1460-x
  5. J. W. Lee, J. H. Do, "Extraction condition of acidic polysaccharide from Korean red ginseng marc". J. Ginseng Res. Vol. 26, pp. 202-205, 2002. DOI: http://dx.doi.org/10.5142/JGR.2002.26.4.202
  6. I. J. Han, R. Y. Kim, Y. M. Kim, C. B. Ahn, D. W. Kim, K. T. Park, S. S. Chun, "Quality characteristics of white bread with red ginseng marc powder". J. East Asian Soc. Dietary Life Vol. 17, pp. 242-249, 2007.
  7. Y. R. Park, I. J. Han, M. Y. Kim, S. H. Choi, D. W. Shin, S. S. Chun, "Quality characteristics of sponge cake prepared with red ginseng marc powder". Korean J. Food Cookery Sci. Vol. 24, pp. 236-242, 2008.
  8. S. D. Kim, J. H. Do, K. S. Lee, H. S. Sung, "Effect of ginseng residue extract on yeast growth". Korean J. Ginseng Res. Vol. 10, pp. 1-10, 1986.
  9. S. D. Kim, J. H. Do, K. S. Lee, "Effect of ginseng residue on the growth of Ganoderma lucidum". Kor. J. Appl. Microbiol. Bioeng. Vol. 14, pp. 279-283, 1986.
  10. D. C, Kim, M. J. In, "Production of hydrolyzed red ginseng residue and its application to lactic acid bacteria cultivation". J. Ginseng Res. Vol. 34, pp. 321-326, 2010. DOI: http://dx.doi.org/10.5142/jgr.2010.34.4.321
  11. C. J. Lee, H. S. Han, C. S. Jhune, J. C. Cheong, J. A. Oh, W. S. Kong, G. C, Park, C. G. Park, Y. S. Shin, "Development of new substrate using red ginseng marc for bottle culture of oyster mushroom (Pleurotus ostreatus)". J. Mushroom Sci. Prod. Vol. 9, pp. 139-144, 2011.
  12. J. K. Hong, M. H. Bong, J. C. Park, H. K. Moon, S. C. Lee, J. H. Lee, S. G. Hwang, "Effect of feeding red ginseng marc on vital reaction in laying hens under stress task". Korean J. Poult. Sci. Vol. 39, pp. 63-70, 2012. DOI: http://dx.doi.org/10.5536/KJPS.2012.39.1.063
  13. I. S. Lee, E. J. Yang, Y. J. Jeong, J. H. Seo, "Fermentation process and physicochemical characteristics of Yakju(Korean cleared rice wine) with addition of ginseng powder". Korean J. Postharvest Sci. Technol. Vol. 4, pp. 463-468, 1999.
  14. G. L. Miller, "Use of dinitrosalicylic acid reagent for determination of reducing sugar". Anal. Chem. 31, pp. 426-428, 1959. DOI: http://dx.doi.org/10.1021/ac60147a030
  15. M. J. In, D. C. Kim, "Fermentation characteristics of wild grape (Vitis amurensis) wine prepared with reed (Phragmites communis) root". J. Korea Acad.-Ind. Cooper. Soc. Vol. 11, pp. 1528-1533, 2010. https://doi.org/10.5762/KAIS.2010.11.4.1528
  16. T. Ando, "Preparation of antilipolytic substance from Panax ginseng". Planta Medica Vol. 38, pp. 18-23, 1980. DOI: http://dx.doi.org/10.1055/s-2008-1074832
  17. H. W. Jung, J. E. Kim, J. H. Seo, S. P. Lee, "Physicochemical and antioxidant properties of red ginseng marc fermented by Bacillus subtilis HA with mugwort powder addition". J. Korean Soc. Food Sci. Nutr. Vol. 39, pp. 1391-1398, 2010. DOI: http://dx.doi.org/10.3746/jkfn.2010.39.9.1391
  18. D. C. Kim, M. J. In, "Potential of red ginseng marc for ethanol production as a fermentation medium". J. Appl. Biol. Chem. Vol. 56, in press, 2013.
  19. J. S. Kim, S. H. Kim, J. S. Han, B. T. Yoon, C. Yook, "Effects of sugar and yeast addition on red wine fermentation using Campbell early". Korean J. Food Sci. Technol. Vol. 31, pp. 516-521, 1999.
  20. H. S. Choi, M. K. Kim, H. S. Park, D. H. Shin, "Changes in physicochemical characteristics of Bokbunja (Rubus coreanus Miq.) wine fermentation". Korean J. Food Sci. Technol. Vol. 37, pp. 574-578, 2005.
  21. E. J. Jeong, Y. S. Kim, D. Y. Jeong, D. H. Shin, "Yeast selection and comparison of sterilization method for making strawberry wine and changes of physicochemical characteristics during its fermentation". Korean J. Food Sci. Technol. Vol. 38, pp. 642-647, 2006.
  22. S. D. Bae, S. M. Bae, J. S. Kim, "Fermentation characteristics of rice-grape wine fermented with rice and grape". Korean J. Food Sci. Technol. Vol. 36, pp. 616-623, 2004.
  23. J. E. Lee, Y. D. Won, S. S. Kim, K. H. Koh, "The chemical characteristics of Korean red wine with different grape varieties". Korean J. Food Sci. Technol. Vol. 34, pp. 151-156, 2002.
  24. S. J. Lee, B. Ahn, "Changes in physicochemical characteristics of black raspberry wines from different regions during fermentation". Korean J. Food Sci. Technol. Vol. 41, pp. 662-667, 2009.
  25. NTSTS Institute, "Textbook of alcoholic beverage-making". pp. 135-136, National Tax Service Technical Service Institute, Seoul, Korea, 1997.
  26. C. K. Park, B. S. Jeon, J. W. Yang, "The chemical components of Korean ginseng". Food Ind. Nutr. Vol. 8, pp. 10-23, 2003.