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반응표면분석을 이용한 청국장 제조시 마늘의 첨가조건 최적화

Optimization of the Addition of Garlic in Cheonggukjang using Response Surface Methodology

  • 투고 : 2013.06.13
  • 심사 : 2013.10.21
  • 발행 : 2013.12.31

초록

마늘을 이용한 청국장의 제조조건을 최적화 하고자 중심합성계획에 따라 마늘의 첨가량($X_1$), 마늘 열처리시간($X_2$) 및 청국장 발효시간($X_3$)을 독립변수로 하고, 점질물 생성량($Y_1$), 산도($Y_2$), 아미노태 질소량($Y_3$), ${\gamma}$-GTP($Y_4$) 및 ABTS 라디칼 소거능($Y_5$)을 종속변수로 하여 반응표면분석을 실시하였다. 점질물 생성량의 정상점은 최대점으로 실제변수인 마늘의 첨가량이 6.53%, 열처리 시간 6.81분, 청국장 발효시간 55.18시간에서 13.02%의 최적 값을 보였다. 산도는 청국장의 발효시간이 길어질수록 증가하였으며, 최소값인 0.50%를 나타내는 마늘 첨가량의 실제변수는 7.75%, 열처리 시간은 3.42분, 청국장 발효는 58.60시간이었다. 아미노태 질소 함량은 80.58~158.82 mg% 범위였는데 정상점은 안장점으로 능선분석을 통해 얻어진 아미노태 질소의 최고값은 156.97 mg%였다. 이 때 마늘 첨가량 및 열처리 시간은 각각 6.21% 및 14.85분, 청국장 발효시간은 58.04시간이었다. ${\gamma}$-GTP 활성은 353.66 mU/mL의 최대값을 가질 때, 마늘 첨가량, 마늘 열처리 시간 및 청국장 발효시간이 각각 5.73%, 6.99분, 57.96시간 이었다. ABTS 라디칼 소거능은 마늘 첨가량과 청국장 발효시간을 낮추고 마늘의 열처리 시간을 높이는 경우 76.43%의 최고값 얻을 수 있었는데, 이에 따른 실제변수의 마늘의 첨가량과 열처리시간은 각각 3.78% 및 14.28분과 청국장 발효시간은 57.99시간이었다. 이상의 결과를 종합하여 볼 때 마늘이 첨가된 청국장 제조를 위해 마늘은 불린 콩 무게 대비 3.78~7.75%로 첨가하되 3.42~14.85분의 범위에서 스팀가열한 후 사용하며, 55~59시간 정도 발효시키는 것이 적합하였다.

This study was performed to determine the optimal composition of Cheonggukjang added with garlic. The experiment utilized a central composite design (CCD). The evaluation was carried out by means of response surface methodology (RSM), which included 18 experimental points with three independent variables : the content of the garlic (1.3~9.7%, $X_1$), the steaming time of garlic (0~15.1 min, $X_2$), and the fermentation time of Cheonggukjang (48.2~71.8 h, $X_3$). The viscous substance ($Y_1$), acidity ($Y_2$), amino-type nitrogen ($Y_3$), ${\gamma}$-GTP activity ($Y_4$) and ABTS radical scavenging activity ($Y_5$). were assessed in four replicates with five dependent variables. The maximum content of the viscous substance was 13.02% at 6.53% ($X_1$), 6.81 min ($X_2$) and 55.18 h ($X_3$). The acidity was increased when the fermentation time was longer, and the minimum acidity point was 0.50% at 7.75% ($X_1$), 3.42 min ($X_2$) and 58.60 h ($X_3$), respectively. The content of the amino-type nitrogen at the experimental range studied was was 80.58~158.82 mg%, and the stationary point was at saddle point. Using ridge analysis, the maximum point was 156.97 mg% at 6.21% ($X_1$), 14.85 min ($X_2$) and 58.04 h($X_3$). The optimum conditions of ${\gamma}$-GTP activity was 5.73% ($X_1$), 6.99 min ($X_2$) and 57.96 h($X_3$), respectively, at the maximum point was 353.66 mU/mL. The maximum point of ABTS radical scavenging activity was 76.43% at 3.78% ($X_1$), 14.28 min ($X_2$) and 57.99 h($X_3$) at the saddle point, when the garlic steaming time was longer.

키워드

참고문헌

  1. Ali M. 1995. Mechanism by which garlic (Allium sativum) inhibits cyclooxygenase activity. Effect of raw versus boiled garlic extract on the synthesis of prostanoids. Prostag Leukotr Ess 53: 397-400 https://doi.org/10.1016/0952-3278(95)90102-7
  2. Ann YG. 2011. Changes in compontents and peptides during fermentation of chunggukjang. Korean J Food Nutr 24: 124-131 https://doi.org/10.9799/ksfan.2011.24.1.124
  3. Bordia T, Mohammed N, Thomson M, Ali M. 1996. An evaluation of garlic and onion as antithrombotic agents. Prostag Leukotr Ess 54: 183-186 https://doi.org/10.1016/S0952-3278(96)90014-9
  4. Bae HJ, Chun HJ. 2002. Changes in volatile sulfur compounds of garlic under various cooking condition. Korean J Soc Food Cookery Sci 18: 365-371
  5. Choi UK, Ji WD, Chung YG. 1998. Characteristics of chunggugjang produced by Bacillus subtilis DC-2. J Korean Soc Food Sci Nutr 27: 846-851
  6. Choi YH, Shim YS, Kim CT, Lee C, Shin DB. 2007. Characteristics of thiosulfinats and volatile sulfur compounds from blanched garlic reacted with allinase. Korean J Food Sci Techonl 39: 600-607
  7. Choe JS, Yoo SM, Kim HI, Kim JS, Chang CM. 1999. Volatile compounds of chungkookjang prepared by different fermentation methods and soybean cultivars. J Korean Soc Agric Chem Biotechnol 42: 111-115
  8. Cavallito CJ, Buck JS, Suter CM. 1944. Allicin the antibacterial principle of Allium sativum: 1. Determination of the chemical structure. J Am Chem Soc 66: 1952-1954 https://doi.org/10.1021/ja01239a049
  9. Hong JY, Kim EJ, Shin SR, Kim TW, Lee IJ. 2008. Physicochemical properties of Chungkookjang containing korean red ginseng and rubus coreanus. Koreoan J Food Preserv 15: 872-877
  10. Hong SC and Kwon DJ. 2011. Changes in quality characteristics of Chungkookjang added with Deodeok. Korean J Food Preserv 18: 171-177 https://doi.org/10.11002/kjfp.2011.18.2.171
  11. Im CM, Kwon SH, Bae MS, Jung KO, Moon SH, Park KY. 2006. Characteristics and increased antimutagenic effect of black soybean (var. Seoritae) chungkukjang. Cancer Prev Res 11: 218-224
  12. Jang EK, Seo JH, Lee SP. 2008. Physiological activity and antioxidative effects of aged black garlic (Allium sativum L.) extract. Korean J Food Sci Technol 40: 443-448
  13. Jung JB, Choi SK, Jeong DY, Kim YS, Kim SY. 2012. Effects of germination time of soybeans on quality characteristics of Cheonggukjang fermented with an isolated bacterial strain. Korean J Food Sci Technol 44: 69-75 https://doi.org/10.9721/KJFST.2012.44.1.069
  14. Jung YJ, Woo SM, Kwon JH, Choi MS, Seong JH, Lee JW. 2007. Quality characteristics of red ginseng Cheonggukjang according to addition methods of red ginseng. J Korean Soc Food Sci Nutr 36: 889-895 https://doi.org/10.3746/jkfn.2007.36.7.889
  15. Jo SJ, Oh SM, Jang EK, Jang EK, Hwang Ki, Lee SP. 2008. Physicochemical properties of carrot juice fermented by Leuconostoc mesenteroides SM. J Korean Soc Food Sci Nutr 37: 210-216 https://doi.org/10.3746/jkfn.2008.37.2.210
  16. Joo HK. 1996. Studies on chemical composition of commercial Chungkookjang and flavor compounds of Chungkookjang by mugwort(Artemisia asiatica) or red paper seed oil. Korean Soybean Digest 13: 44-56
  17. Kim MB, Oh YJ, Lim SM. 2009. Physicochemical characteristic of garlic from daejeong jeju and major cultivation area in korea. The Korean Journal of Culinary research 15: 59-66
  18. Kim JH, Park LY, Lee SH. 2012. Fermentation and quality Characteristics of Cheonggukjang with chinese cabbage. Korean J Food Preserv 19(5): 659-664 https://doi.org/10.11002/kjfp.2012.19.5.659
  19. Kim TS, Choi MK, Kim JS, Han JW, Kang MH. 2009. Screening of lignan compounds and antioxidant activity of Chungkukjang fermented with defatted sesame flour. J Korean Soc Food Sci Nutr 38: 1580-1586 https://doi.org/10.3746/jkfn.2009.38.11.1580
  20. Kim HJ, Han CH, Kim NY, Lee EK, Lee KN, Cho HE, Choi YH, Chong MS. 2010. Effect of garlic extracts with extraction conditions on antioxidant and anticancer activity. Korean J Orintal Physiology & Pathology 24: 111-117
  21. Kang MJ and Shin JH. 2012. Quality characteristics of jochung containing various level of steamed garlic powder. Korean J Food Cookery Sci 28: 865-870 https://doi.org/10.9724/kfcs.2012.28.6.865
  22. Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS. 2006. Physicochemical characteristic of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol 38: 331-336]
  23. Lee BY, Kim DM, Kim KH. 1991. Physico chemical properties of viscous substance extracted from Chungkook-Jang. Korean J Food Sci Technol 23: 599-604
  24. Lee YL, Kim SH, Jung NH, Lim MH. 1992. A study on the production of viscous substance during the chugkookjang fermentation. J Korean Soc Agric Chem Biotechnol 35: 202-209
  25. Lee YR, Woo KS, Hwang IG, Kim HY, Lee SH, Lee JS, Jeong HS. 2012. Physicochemical properties and antioxidant activities of garlic (Allium sativum L.) with different heat and pressure treatments. J Korean Soc Food Sci Nutr 41: 278-282 https://doi.org/10.3746/jkfn.2012.41.2.278
  26. Lee JO, Ha SD, Kim AJ, Yuh CS, Bang IS, Park SH. 2005. Industrial application and physiological functions of Chongkukjang. Food Sci Ind 38: 69-78
  27. Min HK, Kim HJ, Chang HC. 2008. Growth-inhibitory effect of the extract of porphyran-chungkookjang on cancer cell. J Korean Soc Food Sci Nutr 37: 826-833 https://doi.org/10.3746/jkfn.2008.37.7.826
  28. Nishimura H, Hanny W, Mizutani J. 1988. Volatile flavor components and antithrombotic agent: vinyldithinins from Allium victorialis. J Agric Food Chem 36: 563-569 https://doi.org/10.1021/jf00081a039
  29. Park MK. 2011. Effect of enzymatic hydrolysis by proteases on antioxidant activity of chungkukjang. J Korean Soc Food Sci Nutr 40: 327-333 https://doi.org/10.3746/jkfn.2011.40.2.327
  30. Re R, Pellegrini N, Pannala A, Yang M, Rice Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assays. Free Radic Biol Med 26: 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  31. Song JER, You HJ, Hwang JS, Lee DS, Kim HB. 2004. Isolation of angiotensin Ι-converting enzyme inhibitory peptide from chungkookjang. Korean J Microbiol 40: 355-358
  32. Stoll A and Seebeck E. 1951. Chemical investigation on alliin, the specific principle of garlic. Advan Enzymol 11: 377-400
  33. Shin JH, Kang MJ, Kim RJ, Ryu JH, Kim MJ, Lee SJ, Sung NJ. 2011. Biological activity of browning compounds from processed garlics separated by dialysis membrane. J Korean Soc Food Sci Nutr 40: 357-365 https://doi.org/10.3746/jkfn.2011.40.3.357
  34. Shin JH, Choi DJ, Kwen OC. 2007. The quality characteristics of sponge cake with added steamed garlic powder. Korean J Food Cookery Sci. 23: 696-702
  35. Song HS, Kim YM, Lee KT. 2008. Antioxidant and anticancer activities of traditional Kochujang added with garlic porridge. J Life Sci 18: 1140-1146 https://doi.org/10.5352/JLS.2008.18.8.1140
  36. Shin JH, Kang MJ, Yang SM, Lee SJ, Ryu JH, Kim RJ, sung NJ. 2010. Comparison of physicochemical properties and antioxidant activities of Korean traditional kanjang and garlic added kanjang. J Agric Life Sci 44: 39-48
  37. Stewart AJ, Bozonnet S, Mullen W, Jenkins GI, Michael EJ, Croaier A. 2000. Qccurrence of flavonols in tomatoes and tomato-based products. J Agric Food Chem 48: 2663-2669 https://doi.org/10.1021/jf000070p
  38. Youn KC, Kim DH, Kim JO, Park BJ, Yook HS, Cho JM, Byun MW. 2002. Quality characteristics of the chungkookjang fermented by the mixed culture of Bacillus natto and B. licheniformis. J Korean Soc Food Sci Nutr 31: 204-210 https://doi.org/10.3746/jkfn.2002.31.2.204
  39. Yoo SM and Chang CM. 1999. Study on the processing adaptability of soybean cultivars for korean traditional Chungkookjang preparation. J Korean Soc Agric Chem Biotechnol 42: 91-98
  40. Zheng Yanfei, Jeong JK, Choi HS, Park KY. 2011. Increased quality characteristics and physiological effects of Chunggukjang fermented with Bacillus subtilis-SKm. J Korean Soc Food Sci Nutr 40: 1694-1699 https://doi.org/10.3746/jkfn.2011.40.12.1694