동아시아식생활학회지 (Journal of the East Asian Society of Dietary Life)

  • 제23권6호
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  • Pages.704-712
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  • 2013
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  • 1225-6781(pISSN)
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  • 2288-8802(eISSN)
동아시아식생활학회 (The East Asian Society of Dietary Life)
권호 표지

당뇨 유발 흰쥐에서 여주열매 첨가 시 혈당과 콜레스테롤 수준에 미치는 영향

Effect of Bitter Melon on Plasma Blood Glucose and Cholesterol Levels in Streptozotocin Induced Diabetic Rats

  • 김명화 (덕성여자대학교 식품영양학과)
  • Kim, Myung-Wha (Dept. of Food and Nutrition, Duksung Women's University)
  • 투고 : 2013.08.16
  • 심사 : 2013.12.28
  • 발행 : 2013.12.31
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초록

This study was designed to examine the effects of bitter melon (BM) on the plasma blood glucose and cholesterol levels in diabetic rats. Diabetes mellitus was induced in male Sprague-Dawley rats through an injection of streptozotocin (STZ) dissolved in a citrate buffer into the tail vein at a dose of 45 mg/kg of body weight. Sprague-Dawley rats were then fed for four weeks, with the experimental groups receiving a modified diet containing 5% or 10% powder derived from BM. The experimental groups were divided into 4 groups, consisting of the normal control group, STZ-control group and diabetic fed with BM 5% & 10% treated groups. The rats' body weight, blood glucose and cholesterol values were measured along with the hematocrit (Hct) values and aminotransferase activities. Body weight losses were observed in the diabetic groups, whereas the control rats gained weight. There were significant differences in kidney weight between the control group and the diabetic groups. The Hct levels of the diabetic BM-treated group were significantly higher than the STZ-control group. Aspartate aminotransferase activity was lower in the non-diabetic group compared to the diabetic experimental groups. Further, the blood glucose was significantly decreased in the 5% & 10% BM of the diabetic group. There were no significant difference in cholesterol levels among the diabetic groups. These results indicate that the supplementation of bitter melon may have a favorable influence on reducing the blood glucose level in STZ-induced diabetic rats.

키워드

참고문헌

  1. Ahmed I, Lakhani MS, Gillett M, John A, Raza H (2001) Hypotriglyceridemic and hypocholesterolemic effects of antidiabetic Momordica charantia (karela) fruit extract in streptozotocin- induced diabetic rats. Diabetes Res Clin Pract 51: 155-161. https://doi.org/10.1016/S0168-8227(00)00224-2
  2. Allain CC, Poon LS, Chan CS, Richmond W, Pu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20: 470-475.
  3. Bauer JD (1982) Clinical laboratory methods. 9th ed. Mosby Co., St. Louis. pp.188-189.
  4. Brooks DP, Nutting DF, Crofton JT, Share L (1989) Vasopressin in rats with genetic and streptozotocin-induced diabetes. Diabetes 38: 54-57. https://doi.org/10.2337/diab.38.1.54
  5. Choi JW, Sohn KH, Kim SH (1991) The effects of nicotinamide on the serum lipid composition in streptozotocininduced diabetic rats. J Korean Soc Food Nutr 20: 306- 311.
  6. Dai S, McNeill JH (1994) One year treatment of non-diabetic and streptozotocin-diabetic rats with vanadyl sulphate did not alter blood pressure or haematological indices. Pharmacol Toxicol 74: 110-115. https://doi.org/10.1111/j.1600-0773.1994.tb01084.x
  7. Dai S, Thompson KH, McNeill JH (1994) One year treatment of streptozotocin-induced diabetic rats with vanadyl sulphate. Pharmacol Toxicol 74: 101-109. https://doi.org/10.1111/j.1600-0773.1994.tb01083.x
  8. Habicht SD, Kind V, Rudloff S, Borsch C, Mueller AS, Pal lauf J, Yang RY, Krawinkel MB (2011) Quantification of antidiabetic extracts and compounds in bitter gourd varieties. Food Chem 126: 172-176. https://doi.org/10.1016/j.foodchem.2010.10.094
  9. Han HK, Yoon SJ, Kim GH (2009) Effects of compositae plants on plasma glucose and lipid level in streptozotocin induced diabetic rats. J Korean Soc Food Sci Nutr 38: 674-682. https://doi.org/10.3746/jkfn.2009.38.6.674
  10. Hunt JV, Dean RT, Wolff SP (1988) Hydroxyl radical production and autoxidative glycosylation. Biochem J 256: 205- 212. https://doi.org/10.1042/bj2560205
  11. Jeong JH, Lee SH, Hue JJ, Lee KN, Nam SY, Yun YW, Jeong SW, LEE YH, Lee BJ (2008) Effect of bitter melon (Momordica charantia) on anti-diabetic activity in C57BL/ 6J db/db mice. Korean J Vet Res 48: 327-336.
  12. Joseph B (2013) Antidiabetic effects of Momordia charantia (bitter melon) and its medicinal potency. Asian Pac J Trop Dis 3: 93-102. https://doi.org/10.1016/S2222-1808(13)60052-3
  13. Junod A, Lambert AE, Orci L, Picet R, Gonet AE, Renold AE (1967) Studies of the diabetogenic action of streptozotocin. Proc Soc Exp Biol Med 126: 201-205. https://doi.org/10.3181/00379727-126-32401
  14. Khan CR (1985) The molecular mechanism of insulin action. Ann Rev Med 36: 249-251.
  15. Kim BK, Hong JS, Yoon HJ, Hong SD, Hong SP, Lee JI (2013) Influence of bitter melon extraction on oral squamous cell carcinoma. Korean J Oral Maxillofac Pathol 37: 59-66.
  16. Kim JW, Cho HR, Moon SB, Kim KY, Ku SK (2012) Synergic effects of bitter melon and $\beta$-glucan composition on STZinduced rat diabetes and its complications. J Microbiol Biotechnol 22: 147-155. https://doi.org/10.4014/jmb.1106.06037
  17. Kim MW (2010) Effects of sea buckthorn leave on plasma blood glucose and cholesterol level in streptozotocin induced diabetic rats. J East Asian Soc Dietary Life 20: 372-381.
  18. Krawinkel MB, Keding GB (2006) Bitter gourd (Momordica charantia): a dietary approach to hyperglycemia. Nutr Rev 64: 31-337. https://doi.org/10.1111/j.1753-4887.2006.tb00261.x
  19. Lee YH, Kim DJ (2013) Diabetes risk score for Korean adults. J Korean Diabetes 14: 6-10. https://doi.org/10.4093/jkd.2013.14.1.6
  20. Lemhadri A, Hajji L, Michel JB, Eddouks M (2006) Cholesterol and triglycerides lowering activities of caraway fruits in normal and streptozotocin diabetic rats. J Ethnopharmacol 106: 321-326. https://doi.org/10.1016/j.jep.2006.01.033
  21. Lim SJ, Jeong JG, Kim MW, Choi SS, Han HK, Park JE (2003) Effects of Benincasa hispida intake on blood glucose and lipid level in streptozotocin induced diabetic rats. Korean J Nutr 36: 335-343.
  22. Malabu UH, Dryden S, Mccarthy HD, Kilpatrick A (1994) Effect of chronic vanadate administration in the STZ-induced diabetic rats: the antihyperglycemic action of vanadate is attributable entirely to its suppression of feeding. Diabetes 43: 9-15. https://doi.org/10.2337/diab.43.1.9
  23. Matheka DM, Alkizim FO (2012) Glucose-lowering effects of Momordica charantia in diabetic rats. Molecular & Clinical Pharmacol 2: 20-27.
  24. Meena AK, Bansal P, Kumar S (2009) Plants-herbal wealth as a potential source of ayurvedic drugs. Asian Pac J Trop Med 4: 152-170.
  25. Middha SK, Usha T, Tripathi P, Marathe KY, Jain T, Bhargavi B, Masurkar YP, Pande V (2012) An in vitro studies on indigenous ayurvedic plants, having hypoglycemic activity. Asian Pac J Trop Dis 2: S46-S49. https://doi.org/10.1016/S2222-1808(12)60122-4
  26. Noguchi R, Yasui Y Noguchi R, Yumiko Yasui Y, Suzuki R, Hosokawa M, Fukunaga K, Miyashita K (2001) Dietary effects of bitter gourd oil on blood and liver lipids of rats. Archives Biochemistry and Biophysics 396: 207-212. https://doi.org/10.1006/abbi.2001.2624
  27. O'Meara NM, Devery RA, Owens D, Collins PB, Johnson AH, Tomkin GH (1990) Cholesterol metabolism in alloxaninduced diabetic rabbits. Diabetes 39: 626-633. https://doi.org/10.2337/diab.39.5.626
  28. Park JY, Heo YR (2011) Effects of bitter melon (Momordica charantia) extracts on Body weight change and lipid composition in C57/BL6J mice fed high fat diet. J Human Ecol 21: 113-121.
  29. Park SA (2013) Social welfare approach for the patient with diabetic nephropathy. J Korean Diabetes 14: 42-45. https://doi.org/10.4093/jkd.2013.14.1.42
  30. Park Y, Boo HO, Park YL, Cho DH, Lee HH (2007) Antioxidant activity of Momordica charantia L. extracts. Korean J Medicinal Crop Sci 15: 56-61.
  31. Pawlak DB, Kushner JA, Ludwig DS (2004) Effects of dietary glycaemic index on adiposity, glucose homoeostasis, and plasma lipids in animals. Lancet 364: 778-785. https://doi.org/10.1016/S0140-6736(04)16937-7
  32. Platel K, Srinivasan K (1997) Plant foods in the management of diabetes mellitus: vegetables as potential hypoglycaemic agents. Nahrung 41: 68-74. https://doi.org/10.1002/food.19970410203
  33. Raabo E, Terkildsen TC (1960) On the enzymatic determination of blood glucose. Scand J Lab Invest 12: 402-407. https://doi.org/10.3109/00365516009065404
  34. Reeves PG (1997) Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 127: 838-841. https://doi.org/10.1093/jn/127.5.838S
  35. Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28: 56-63. https://doi.org/10.1093/ajcp/28.1.56
  36. Robert E, Mainous AG, King DE, Simpson KN (2012) Dietary fiber for the treatment of type 2 diabetes mellitus: a meta-analysis. JABAFM 25: 16-28. https://doi.org/10.3122/jabfm.2012.01.110148
  37. Senanayake GVK, Maruyama M, Shibuya K, Sakono M, Fukuda N, Morishita T, Yukizaki C, Kawano M, Ohta H (2004) The effects of bitter melon (Momordica charantia) on serum and liver triglyceride levels in rats. J Ethnopharmacol 91: 257-262. https://doi.org/10.1016/j.jep.2003.12.026
  38. Seo KH, Choi JS, Na JO, Uh ST, Kim YH, Park CS (2006) Expression of matrix metalloproteinase-9 and tissue in hibitor of metalloproteinase-1 after administration of endotoxin in diabetic rats. Tuberc Respir Dis 61: 256-264. https://doi.org/10.4046/trd.2006.61.3.256
  39. Sochor M, Kunjara S, Baquer NZ, Malean P (1991) Regulation of glucose metabolism in livers and kidneys of NOD mice. Diabetes 40: 1467-1471. https://doi.org/10.2337/diab.40.11.1467
  40. Subratty AH, Gurib-Fakim A, Mahomoodally F (2005) Bitter melon: an exotic vegetable with medicinal values. Nutrition & Food Science 35: 143-147. https://doi.org/10.1108/00346650510594886
  41. Tamariz LJ, Young JH, Pankow JS, Yeh HC, Schmidt MI, Astor B, Brancati FL (2008) Blood viscosity and hematocrit as risk factors for type 2 diabetes mellitus the atherosclerosis risk in communities (ARIC) study. Am J Epidemiol 168: 1153-1160. https://doi.org/10.1093/aje/kwn243
  42. Wannamethee SG, Perry IJ, Shaper AG (1996) Hematocrit and risk of NIDDM. Diabetes 45: 576-579. https://doi.org/10.2337/diab.45.5.576
  43. Wilson GL (1984) Mechanism of streptozotocin-induced and alloxan-induced damage in rat $\beta$-cells. Diabetologia 27: 587-591. https://doi.org/10.1007/BF00276973
  44. Yang YR, Kim HL, Park YK (2008) Effects of onion kimchi extract supplementation on blood glucose and serum lipid contents in streptozotocin-induces diabetic rats. J Korean Soc Food Sci Nutr 37: 445-451. https://doi.org/10.3746/jkfn.2008.37.4.445
  45. http://ndb. nal.usda.gov/ndb/search/list. Accessed September 28, 2013.