Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Krill Euphausia superba Fluoride Extract on Toxicity and Oxidative Stress in Liver cell

크릴(Euphausia superba) 불소 추출물의 간세포 독성 및 산화적 스트레스에 미치는 영향

  • Kim, Jeong Gyun (Department of Food Science & Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Yoon, Ho Dong (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Park, Sihyang (Sun marine Biotechnology Co.) ;
  • Kim, Poong Ho (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Mok, Jong Soo (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Hong, Yumi (Sun marine Biotechnology Co.)
  • 김정균 (경상대학교 해양과학대학 해양식품공학과) ;
  • 윤호동 (국립수산과학원 남동해수산연구소) ;
  • 박시향 (선마린바이오테크) ;
  • 김풍호 (국립수산과학원 남동해수산연구소) ;
  • 목종수 (국립수산과학원 남동해수산연구소) ;
  • 홍유미 (선마린바이오테크)
  • Received : 2013.09.09
  • Accepted : 2013.11.20
  • Published : 2013.12.31
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Abstract

In this study, we investigated about cell toxicity and oxidative stress of HepG2 cell by treatment of sodium fluoride (NaF) and fluoride extracts from krill Euphausia superba meat, shell, whole body and krill meal. The cell toxicity showed significant at 300 and $500{\mu}g/mL$ NaF treatment group. But krill (Euphausia superba) fluoride extract (KFE) treatment in all groups were not toxic. The superoxide radical production increased significantly in NaF treated group, but there was no significant change in KFE treated group. The superoxide dismutase activity was a significant increase 21.5% at $100{\mu}g/mL$ and 24.7% at $300{\mu}g/mL$ treatment group of fluoride extracts from krill meat, and 8.7% at $300{\mu}g/mL$ in krill meals, compared to the control group. However, hydroxy radical flux and catalase and glutathione peroxidase activity of fluoride extracts from krill meat did not change. As a result, for a short period of time, NaF treatment in HepG2 cells affect the cell toxicity and oxidative stress, but in the case of KFE, these were not recognized. Thus, depending on the type of food ingested with fluoride, cell toxicity and oxidative stress was found to be different.

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References

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