Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Survival and Histological Change of Integumentary System of the Juvenile Sea Cucumber, Apostichopus japonicus Exposed to Various Salinity Concentrations

염분 농도에 따른 어린 돌기해삼, Apostichopus japonicus의 생존 및 피부계의 조직학적 변화

  • Kim, Tae-Ik (Southwest Sea Fisheries Research Institute, NFRDI) ;
  • Park, Min-Woo (Southwest Sea Fisheries Research Institute, NFRDI) ;
  • Cho, Jae-Kwon (Southwest Sea Fisheries Research Institute, NFRDI) ;
  • Son, Maeng-Hyun (Southwest Sea Fisheries Research Institute, NFRDI) ;
  • Jin, Young-Guk (Southwest Sea Fisheries Research Institute, NFRDI)
  • 김태익 (국립수산과학원 남서해수산연구소) ;
  • 박민우 (국립수산과학원 남서해수산연구소) ;
  • 조재권 (국립수산과학원 남서해수산연구소) ;
  • 손맹현 (국립수산과학원 남서해수산연구소) ;
  • 진영국 (국립수산과학원 남서해수산연구소)
  • Received : 2013.10.24
  • Accepted : 2013.12.04
  • Published : 2013.12.31
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Abstract

Juvenile sea cucumber, Apostichopus japonicus(wet weight $1.0{\pm}0.2g$, n=250) acclimated to environmental conditions($17^{\circ}C$, 33 psu) during 4 weeks for observation of survival and structural variation of integumentary system according to various salinity concentration. After that, it was exposed 0, 5, 10, 15, 20, 25, 30, 35, 40 psu in 96h, and recovered during 7 days. In this results, The 96-h lethal concentration(LC50) for sea cucumber was a salinity of 21.05 psu. Integumentary system of the sea cucumber exposed to below the salinity of 20 psu mainly observed thickness reduction of epidermal layer, nucleus condensation of epithelial cells, decreased of mucous cells and loose arrangement of connective tissue in dermal layer. Integumentary system of the sea cucumber exposed a salinity of 40 psu mainly observed nucleus hypertrophy of epithelial cells, increase of mucous cells and tight arrangement of connective tissue in dermal layer. Also, observed secretory cells showing the alcian blue(pH 2.5) positive. During 96-h exposure, this cells decreased after increase below the salinity of 20 psu but increased in the salinity of 40 psu compared with 25-35 psu.

다양한 염분 농도에 따른 어린 돌기해삼의 생존과 피부계의 구조적 이상을 관찰하였다. 돌기해삼의 크기 및 수는 습중량 $1.0{\pm}0.2g$, 250 개체였다. 실험농도는 0, 5, 10, 15, 20, 25, 30, 35 및 40 psu 였으며, 96시간 노출 후 7일간 일반해수에서 회복과정을 통해 생존을 관찰하였다. 그 결과 25-35 psu에 노출된 개체들은 모두 100%의 생존율을 보였으며, LC50은 21.05 psu로 나타났다. 돌기해삼의 피부계는 20 psu 이하의 염분 농도에 노출된 개체들의 경우 상피층 두께 감소, 상피세포 핵 응축, 점액세포 감소 및 진피층 결합조직의 성긴 배열 등이 주로 관찰되었다. 하지만 이와는 반대로 40 psu의 염분 농도에 노출된 개체들은 상피세포 핵의 비대, 점액세포 증가 및 진피층 결합조직의 치밀배열 등이 나타났다. 진피층에서 관찰된 alcian blue(pH 2.5) 양성 분비세포들은 20 psu 이하에서는 노출 24시간 내에 대조구에 비해 급격한 증가 및 감소폭을 보였다. 40 psu 에서는 노출시간이 길어질수록 증가한 후 회복일수에 따른 감소경향을 보였다.

Keywords

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